Recombinant Human GNAS Protein: FAQs & Key Facts

Recombinant Human GNAS Protein is a recombinant form of the stimulatory G-protein alpha subunit (Gsα) encoded by the GNAS gene, widely used in research investigating adenylyl cyclase activation, cAMP-mediated signal transduction, and diseases associated with GNAS mutations.

Product Name

Recombinant Human GNAS Protein

SKU

rp368007

Gene / Protein

GNAS (Guanine Nucleotide-binding protein G(s) subunit alpha)

Primary Function

Stimulates adenylyl cyclase to increase intracellular cAMP levels upon GPCR activation

Key Research Areas

G-protein signaling, cAMP pathway, endocrinopathies, oncology, McCune–Albright syndrome

Supplier Reference

absea#PP-2119

What Is Recombinant Human GNAS Protein?

The GNAS gene encodes the alpha subunit of the stimulatory heterotrimeric G-protein (Gsα), a critical mediator in G-protein-coupled receptor (GPCR) signaling. Upon receptor activation, Gsα exchanges GDP for GTP, dissociates from the Gβγ dimer, and directly stimulates adenylyl cyclase, leading to increased intracellular cyclic AMP (cAMP) production.

Recombinant Human GNAS Protein (SKU: rp368007) is a recombinantly produced version of this protein, enabling researchers to study its biochemical properties, protein–protein interactions, and enzymatic regulation in a controlled in vitro setting, without the variability associated with native tissue-derived preparations.

GNAS-related research is highly relevant to endocrinology, oncology, and rare disease biology. Gain-of-function mutations in GNAS are associated with McCune–Albright syndrome and several endocrine tumors, while loss-of-function mutations underlie pseudohypoparathyroidism. The recombinant protein provides a consistent tool for mechanistic and translational studies in these areas.

What Are the Recommended Applications for GNAS Protein?

Recombinant Human GNAS Protein is suitable for a range of biochemical and cell-free research applications, including but not limited to:

• Biochemical assays: GTPase activity assays, adenylyl cyclase stimulation assays, and GTP/GDP binding studies to characterize Gsα function.
• Protein–protein interaction studies: Pull-down assays, co-immunoprecipitation validation, and surface plasmon resonance (SPR) experiments examining Gsα interactions with GPCRs, adenylyl cyclase isoforms, or regulatory proteins.
• Structural biology: Use as an antigen for antibody generation or validation, or as a component in structural studies (e.g., cryo-EM complexes).
• Drug discovery: Screening for small molecules that modulate Gsα activity or its interactions, relevant to therapeutic targets in endocrine disorders and cancers harboring GNAS mutations.
• Western blot positive control: As a defined recombinant standard to validate GNAS antibody specificity.

Researchers should confirm suitability for each specific assay format by consulting the product datasheet or contacting Aladdin Scientific technical support.

How Should Recombinant Human GNAS Protein Be Stored and Handled?

Proper storage and handling are critical to maintaining the activity and integrity of recombinant proteins. As general best-practice guidelines applicable to recombinant Gsα proteins:

• Short-term storage (≤1 month): Store at 4 °C in the supplied buffer.
• Long-term storage: Aliquot and store at −20 °C or −80 °C to avoid repeated freeze–thaw cycles, which can cause protein aggregation and loss of activity.
• Carrier protein: If the protein is supplied without a stabilizing carrier (e.g., BSA), consider adding glycerol (to 10–50%) or a suitable carrier protein before freezing.
• Handling: Keep on ice during use; minimize exposure to temperatures above room temperature. Avoid vortexing; mix gently by pipetting.

Note: Always refer to the specific product datasheet provided with SKU rp368007 for the exact buffer composition, recommended storage temperature, and shelf-life information, as formulation details may differ from general guidelines.

What Is the Biological Significance of GNAS in Disease Research?

The GNAS locus is one of the most complex in the human genome, subject to genomic imprinting and giving rise to multiple transcripts and protein isoforms. Dysregulation or mutation of GNAS is implicated in a spectrum of human diseases:

• McCune–Albright Syndrome: Caused by somatic gain-of-function mutations (most commonly R201H or R201C) in GNAS, resulting in constitutive Gsα activity, elevated cAMP, and autonomous activation of hormone-responsive tissues (bone, skin, endocrine glands).
• Endocrine Tumors: Activating GNAS mutations are found in a subset of pituitary somatotroph adenomas (growth hormone-secreting tumors), thyroid adenomas, and pancreatic intraductal papillary mucinous neoplasms (IPMNs).
• Pseudohypoparathyroidism (PHP): Loss-of-function mutations or imprinting defects at GNAS cause resistance to parathyroid hormone (PTH) and other hormones signaling through cAMP.
• Fibrous Dysplasia: A skeletal disorder driven by the same activating GNAS mutations as McCune–Albright syndrome.

Recombinant GNAS protein enables researchers to model wild-type and mutant Gsα biochemistry in vitro, supporting mechanism-of-disease studies and drug target validation.

Ordering, Quality, and Technical Support

Recombinant Human GNAS Protein is available from Aladdin Scientific under SKU rp368007. The following information is relevant for procurement and quality assessment:

• Quality assurance: Recombinant proteins from Aladdin Scientific undergo standard quality-control testing. Researchers are advised to request the Certificate of Analysis (CoA) and product datasheet at the time of ordering for lot-specific purity, concentration, and activity data.
• Ordering: Place orders through the Aladdin Scientific website or contact your regional Aladdin Scientific representative. Reference SKU rp368007 and supplier reference absea#PP-2119 when ordering or requesting a quote.
• Technical support: For application-specific guidance, protocol optimization, or questions about suitability of this product for your assay, contact Aladdin Scientific technical support directly. Our team can assist with storage recommendations, reconstitution protocols, and troubleshooting.
• Returns and refunds: Refer to Aladdin Scientific's standard returns policy for reagent products. Contact customer service with your order details if the product does not meet the stated specifications.

Frequently asked questions

What is the function of the GNAS protein encoded by the human GNAS gene?

The GNAS gene encodes the alpha subunit of the stimulatory G-protein (Gsα), which couples activated G-protein-coupled receptors (GPCRs) to adenylyl cyclase. Upon receptor stimulation, Gsα exchanges GDP for GTP, dissociates from the Gβγ complex, and activates adenylyl cyclase to produce cyclic AMP (cAMP), a second messenger regulating numerous cellular processes including metabolism, cell proliferation, and hormone secretion.

What are the main diseases associated with GNAS mutations, and how does this protein help study them?

Gain-of-function mutations in GNAS are associated with McCune–Albright syndrome, fibrous dysplasia, pituitary somatotroph adenomas, thyroid adenomas, and pancreatic IPMNs, while loss-of-function alterations cause pseudohypoparathyroidism. Recombinant Human GNAS Protein allows researchers to reconstitute wild-type or mutant Gsα biochemistry in vitro, enabling mechanistic studies of constitutive activation, GTPase kinetics, and protein interactions relevant to these conditions.

How should I store Recombinant Human GNAS Protein (rp368007) to preserve its activity?

As a general guideline, recombinant proteins should be aliquoted upon receipt and stored at −20 °C or −80 °C for long-term preservation, avoiding repeated freeze–thaw cycles that can compromise protein integrity and activity. For short-term use within one month, storage at 4 °C in the supplied buffer is typically acceptable. Always refer to the specific product datasheet for SKU rp368007 for lot-specific storage conditions and expiry information.

What research applications is Recombinant Human GNAS Protein suitable for?

Recombinant Human GNAS Protein (rp368007) is suitable for biochemical assays including GTPase activity measurements and adenylyl cyclase stimulation assays, protein–protein interaction studies (pull-down, SPR), Western blot positive control applications, antigen use for antibody validation, and drug discovery screening targeting Gsα modulation. Researchers should confirm compatibility with their specific assay format by consulting the product datasheet or Aladdin Scientific technical support.

How do I order Recombinant Human GNAS Protein from Aladdin Scientific?

Recombinant Human GNAS Protein can be ordered directly through the Aladdin Scientific website or by contacting your regional representative. Reference SKU rp368007 and supplier reference absea#PP-2119 when placing your order or requesting a quotation. For bulk quantities or custom formulations, contact Aladdin Scientific customer service or technical support for assistance.

References

1. Weinstein LS, et al. Genetics, therapy and potential mechanisms of disease involving the GNAS locus. Nature Reviews Endocrinology. (General reference — specific volume/page details not provided in source material.)
2. Gilman AG. G proteins: transducers of receptor-generated signals. Annual Review of Biochemistry. 1987;56:615–649.
3. Aladdin Scientific. Recombinant Human GNAS Protein, SKU rp368007. Product datasheet. (Supplier reference: absea#PP-2119.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.