L-369 , CAS No.1443522-24-4

CAS: 1443522-24-4 Cat. No.: L1439964 Fórmula: C₄₉H₉₅NO₆ Peso molecular: 794.28

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Visão geral

L-369 is an ionizable cationic lipid that can be used to synthesize lipid nanoparticles and deliver mRNA.

Specifications

Condições de armazenamento de armazenamento

Store at -20°C

Enviado em

Ice chest + Ice pads

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Nomes e identificadores

Peso molecular

794.28

Application Protocols

Item-specific validated applications

• Not specified for this item; refer to CoA/Spec Sheet.

General small-molecule handling for assays (non-product-specific)

• Stock preparation:
  • Weigh quickly to minimize moisture uptake. Prepare a 10–50 mM stock in an appropriate solvent (commonly DMSO). Vortex and sonicate as needed. If particulates persist, warm gently (≤40 °C) and/or filter (0.22 µm PTFE/PVDF).
  • Aliquot into low-bind microtubes (e.g., 10–50 µL) and store at −20 °C to avoid repeated freeze–thaw.
• Serial dilution:
  • Prepare intermediate dilutions in DMSO to limit aqueous precipitation, then step into assay buffer to reach final concentrations. Maintain consistent final solvent percentage across controls and samples.
• Assay setup:
  • Include vehicle controls (matching solvent %), positive/negative controls where available, and run in technical replicates. Pre-clear solutions by centrifugation if turbidity is observed.
• Plate handling:
  • Use polypropylene plates for DMSO stocks. Minimize edge effects by equilibrating plates to room temperature before incubation. Seal plates to reduce evaporation in long assays.
• Data integrity:
  • Record lot number, stock concentration, solvent, preparation date, and storage history. Re-verify concentration periodically by UV or LC–MS if the compound is used over extended periods.

Safety

• Perform all handling in a fume hood with appropriate PPE. This product is for research use only.

Biological Roles

Item-specific biological information

• No target, pathway, or mechanism data are provided for L-369 in the current Product Data. This entry is listed as a small molecule for research use only.

General context for library compounds (non-product-specific)

• Small molecules in screening libraries may serve as starting points for identifying modulators of enzymes, receptors, ion channels, transporters, or signaling pathways. Assignment of a biological role typically follows internal screening data, orthogonal confirmation, and preliminary selectivity profiling.
• If a target association becomes known, characterize mode of action (e.g., orthosteric vs. allosteric modulation, covalent vs. reversible binding) through biochemical kinetics, competition assays, thermal shift/DSF, and cellular target engagement techniques.
• Consider adsorption and nonspecific binding: Highly lipophilic or aromatic compounds may adhere to plastics or serum proteins, affecting apparent potency; employ appropriate controls and low-binding consumables.

Important

• Without explicit target information, avoid extrapolating biological roles from the product name. Use this item strictly for research and method development, and document assay conditions thoroughly to enable reproducibility.

Buffer Applications

Applicability to buffers

• L-369 is a small-molecule library member; it is not a buffering agent. However, it may be formulated into buffers for biochemical or cell-based assays.

General guidance for preparing assay solutions (non-product-specific)

• Stock solutions: Prepare 10–50 mM stocks in DMSO when solubility allows. Store frozen in small aliquots to avoid freeze–thaw.
• Dilution into aqueous buffers: Add DMSO stocks to target buffer (e.g., PBS, HEPES, Tris) with vigorous mixing to minimize local supersaturation. Typical final DMSO levels tolerated:
  • Enzymatic biochemical assays: 0.1–2% v/v (method-dependent).
  • Mammalian cells: 0.1–0.5% v/v preferred; up to 1% v/v often acceptable depending on cell type.
• Common buffers by pH window (general info):
  • Acetate (pH ~3.6–5.6), MES (pH ~5.5–6.7), MOPS/HEPES (pH ~6.5–8.0), Tris (pH ~7.0–9.0).
• Precipitation check: After dilution, visually inspect and, if needed, centrifuge or filter. Validate concentration by UV or LC where feasible.

Notes

• Avoid strong acid/base in the absence of stability data. Include solvent-only controls to account for DMSO effects.
• No item-specific buffer compatibility or pKa data are provided; consult the CoA/Spec Sheet if available.

Green Alternatives

Because L-369’s solubility and reactivity are not specified, solvent choice should balance solvency needs with greener chemistry principles when preparing stocks or conducting assays.

Greener dissolution and handling options (general)

• Prefer DMSO for stock solutions when compatible: low volatility, relatively benign profile, excellent solvency.
• Favor bio-based or lower-toxicity alcohols (ethanol, isopropanol) over methanol or halogenated solvents where feasible.
• Minimize DMF and NMP due to reproductive toxicity; if high solvency is required, consider alternatives like Cyrene (dihydrolevoglucosenone) or GBL after compatibility checks.

Illustrative comparison (general, not item-specific)

• DMSO: High solvency, miscible with water; low vapor pressure; widely accepted in bioassays.
• Ethanol: Renewable sources available; good for polar compounds; higher volatility may aid drying.
• Acetonitrile: Useful analytically; moderate toxicity; good UV transparency.
• Ethyl acetate/2-MeTHF/CPME: Potential workup/crystallization solvents with improved EHS versus chlorinated solvents; suitability depends on compound polarity.

Operational green tips

• Use small-volume, high-concentration stocks to reduce solvent consumption.
• Employ closed systems and cold traps to limit VOC emissions during solvent removal.
• Validate that greener substitutes do not compromise assay performance (solvent tolerance, precipitation risk).

Note

• No specific solvent restrictions or stability constraints are provided for this item; confirm compatibility experimentally.

Pharmaceutical Uses

Item-specific status

• No pharmacopeial status, excipient role, or formulation data are provided for L-369. This product is designated for research use only and is not intended for human or veterinary use.

General context (non-product-specific)

• Library small molecules may be used in discovery-stage research, including assay development, screening, and preliminary formulation feasibility studies (e.g., solubility enhancement strategies) within nonclinical laboratories.
• Any progression toward pharmaceutical development requires comprehensive CMC characterization, impurity profiling, solid-form screening (polymorph/salt/co-crystal), and stability studies—none of which are provided here.

Compliance note

• Do not use this material in clinical applications, diagnostic procedures, or as an API/excipient without appropriate regulatory documentation and qualification. For any GLP/GMP needs, request appropriate grade and documentation; this listing does not specify such qualifications.

Physical Properties

Item-specific facts (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

Typical physicochemical parameters (not item-specific; provide only when disclosed)

• Melting point, boiling point, density, refractive index, pKa, logP/logD, water/organic solubility: Not specified for this item; refer to CoA/Spec Sheet.

Practical guidance for initial characterization (general best practice)

• If properties are not provided, perform a minimal panel to guide handling and dissolution:
  • Differential scanning or simple capillary melting point (if solid) to assess thermal behavior.
  • Rapid LC–UV/ESI-MS to verify purity and mass; check for solvent adducts or salts.
  • Solubility screen at room temperature in DMSO, DMF, methanol, acetonitrile, ethanol, isopropanol, water, and PBS (with/without small % DMSO).
  • If hygroscopicity is suspected, weigh in a dry box or quickly under low humidity and record mass drift.
• Filtration note: Where visible particulates persist, pass solutions through a 0.22 µm PTFE or PVDF syringe filter compatible with the diluent.
• Stability reconnaissance: Perform a short-term stability check (e.g., 24–72 h at ambient and refrigerated, in light vs. dark) by LC–UV to detect degradation or isomerization. Protect from moisture and light until specific stability data are available.

Reminder

• Treat any uncharacterized small molecule as potentially sensitive to hydrolysis, oxidation, and photolysis until item-specific data are obtained.

Quality and Grades

Item-specific information

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.

Understanding typical designations (general guidance)

• Research grade: Suitable for exploratory R&D; purity and impurity profiles may be specified by HPLC area % and/or LC–MS.
• Screening/Library compounds: Often supplied with target purity (e.g., ≥90–98% by HPLC) and may be provided as free base or salt; verify the exact specification on the CoA.
• Stabilizers/additives: If present, they will be stated on the CoA/Spec Sheet. None are indicated in the Product Data.

What to check on receipt (best practices)

• Identity confirmation: Verify by LC–MS (expected m/z), and, if necessary, 1H NMR.
• Purity: Review HPLC chromatogram and method parameters (column, gradient, detection wavelength). If UV cutoff matters for your application, consult the Spec Sheet.
• Water/volatile content: If Karl Fischer or LOD is relevant, check the CoA; not specified in this listing.
• Salt form and counter‑ion content: Confirm if the compound is supplied as a salt; neutralize calculations accordingly for solution preparation.

Documentation

• For regulated workflows, request a CoA with lot-specific data. If analytical grade or higher is required (e.g., LC–MS grade solvents), ensure the grade aligns with your application. For L-369, only research use is stated in the Product Data.

Reaction and Applications

Item-specific intended use

• The product is categorized under small molecules/compound libraries and marked for research use only. No specific synthetic or mechanistic application is disclosed in the Product Data.

General applications for library compounds (non-product-specific)

• Screening/reference tool in target-based or phenotypic assays to explore activity space, build SAR hypotheses, or benchmark assay performance (e.g., positive/negative control candidates, once activity is assigned internally).
• Probe development workflows: Hit validation, secondary assays, preliminary DMPK assessments, and selectivity panels.
• Analytical method development: System suitability challenges for LC–MS methods (retention behavior, ionization response) when structural data are available.

Practical considerations

• Prepare a stability-indicating LC method before extensive testing. Monitor for hydrolysis/oxidation across assay-relevant buffers and temperatures.
• If the compound is light- or air-sensitive (unknown here), work under inert atmosphere and use amberware. Implement aliquoting to minimize freeze–thaw.
• Avoid reactive derivatization or coupling chemistry unless the structure and functional groups are confirmed; many library compounds contain motifs that can be incompatibly reactive under standard conditions.

Limitations

• Without structure or functional group information, specific reaction families (e.g., cross-coupling, condensations) cannot be recommended for this item. Refer to the CoA/Spec Sheet for functional group guidance if synthetic transformations are intended.

Reaction Conditions

Item-specific reaction conditions

• Not applicable: No functional group or reactivity information is provided for L-369. Reaction temperatures, catalysts, bases/acids, and solvent systems cannot be recommended without structure.

General notes (non-product-specific)

• If structure becomes available and derivatization is planned, establish:
  • Solvent and base/acids compatibility via small-scale scouts.
  • Temperature limits based on differential scanning calorimetry or observed decomposition in TLC/LC–MS.
  • Sensitivity to oxygen/light/water, employing inert gas and amberware as needed.
• Analytical monitoring: Use LC–MS and orthogonal detection (UV/ELSD/MS) to track starting material consumption, product formation, and impurities.

Yield expectations

• No item-specific yields or literature precedents are available for L-369. Consult literature once the scaffold is identified.

Safety and Handling

Item-specific hazard information

• Signal word: Not specified for this item; refer to SDS.
• H-statements: Not specified for this item; refer to SDS.
• GHS classification and pictograms: Not specified for this item; refer to SDS.

General laboratory precautions for small molecules (not product-specific)

• Handle in a chemical fume hood. Avoid inhalation of dust/vapors and skin/eye contact.
• Wear appropriate PPE: lab coat, safety glasses, and suitable chemically resistant gloves (e.g., nitrile). Change gloves frequently during weighing/dissolution.
• Avoid generating aerosols or fine dust. Use antistatic measures for dry powders.
• Incompatibilities: Unknown for this item. Until known, avoid strong oxidizers/reductants, strong acids/bases, and moisture. Use amber glass if photolability is suspected.
• First aid (general):
  • Skin/eye: Rinse with water for at least 15 minutes; remove contaminated clothing; seek medical attention if irritation persists.
  • Inhalation: Move to fresh air; obtain medical attention if symptoms occur.
  • Ingestion: Rinse mouth; do not induce vomiting; seek medical attention.
• Spill response: Absorb with inert material (e.g., vermiculite), collect in a suitable waste container, and decontaminate area. Prevent entry into drains.
• Waste disposal: Dispose of as organic chemical waste per institutional and local regulations.

Storage and transport (item-specific)

• Store at −20°C (per Product Data) in tightly closed, chemically compatible containers; minimize headspace and moisture ingress.
• Shipped in an ice chest with ice pads. Inspect upon arrival; return to −20°C promptly.

Important

• SDS is the authoritative source for hazard classifications and first-aid measures. This product is for research use only.

Solvent Selection

Item-specific solubility: Not specified for this item; refer to CoA/Spec Sheet.

General approach for small-molecule dissolution

• Start with high-permissivity, polar aprotic solvents: DMSO and DMF frequently dissolve diverse chemotypes at 10–100 mM.
• Secondary options: Methanol, acetonitrile, ethanol, isopropanol. If ionic or strongly polar, water or buffered saline with 2–10% DMSO/cosolvent may be feasible.
• Stepwise screening: Test 1–2 mg in 100–200 µL at room temperature, sonicate 5–10 min, then warm gently (≤40 °C) as needed. Avoid prolonged heating without stability data.

Miscibility and practical notes (general)

• DMSO: Fully miscible with water and most organics; low volatility; widely used for 10–50 mM stocks.
• DMF: Strong solvent; miscible with water/organics; handle with care due to toxicity.
• Methanol/Ethanol: Protic, volatile; good for polar compounds; often acceptable for bioassays at low final %.
• Acetonitrile: Polar aprotic; UV transparent; good for LC applications.

Selection by use case

• Biochemical assays: Prepare concentrated DMSO stock, dilute into assay buffer; keep final DMSO ≤0.1–1% v/v as assay tolerance permits.
• LC/analytical prep: Choose diluent matched to mobile phase (e.g., water/acetonitrile with 0.1% FA) to minimize peak distortion.
• Crystallization: If characterization is needed, identify antisolvents by small-scale solvent/antisolvent trials.

Caveats

• Avoid reactive media (strong acid/base) until compatibility is known. Filter solutions through 0.22 µm PTFE/PVDF if particulates persist.

Storage and Reconstitution

Item-specific storage and shipping

• Storage: Store at −20 °C (per Product Data). Keep container tightly closed in a dry, well-ventilated place. Protect from moisture; protect from light if sensitivity is suspected.
• Shipping: Shipped in an ice chest with ice pads. On receipt, inspect packaging, allow to equilibrate briefly to ambient to avoid condensation, then return to −20 °C promptly.

Reconstitution guidance (general, non-item-specific)

• Solvent selection: Begin with dry DMSO for primary stocks; consider DMF, methanol, ethanol, or acetonitrile if DMSO is unsuitable. Item-specific solubility is not provided; confirm experimentally.
• Concentration: 10–50 mM stocks are typical for library compounds, adjusted to molecular weight and solubility. If the salt form is unknown, express concentration in mass/volume (mg/mL) until confirmed.
• Technique: Vortex thoroughly, sonicate 5–10 minutes, and gently warm (≤40 °C) if necessary. Filter through a 0.22 µm PTFE/PVDF filter to remove particulates if required.

Aliquoting and stability

• Prepare single-use aliquots (e.g., 10–50 µL) to avoid freeze–thaw cycles. Record preparation date and storage history. For working solutions in aqueous buffer, keep on ice and use the same day unless stability is verified.
• Long-term stability: Not specified for this item; refer to CoA/Spec Sheet. Until stability is known, limit storage to ≤6 months at −20 °C for concentrated DMSO stocks and inspect by LC–MS/HPLC before reuse.

Important

• No grade/purity, stabilizers, or exact identity are provided; consult the CoA/Spec Sheet and SDS before critical experiments.

Structure and Identity

Item-specific facts (from Product Data)

• Product name: L-369 (SKU: L1439964)
• CAS: 1443522-24-4
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

Structural features

• Structural class/type: Not disclosed in the Product Data. The compound is listed under “small molecules and compound libraries,” suggesting use as a screening/reference small molecule.
• Functional groups, ring systems, stereochemistry: Not specified for this item; refer to CoA/Spec Sheet.

2D structure (descriptive)

• A 2D depiction cannot be provided because the structural identifiers (SMILES/InChI) are not included in the current listing. Please consult the CoA/Spec Sheet or SDS for structural information when available.

Notes for users

• If structural data are required for cheminformatics, docking, or analytical confirmation, request the CoA/Spec Sheet or contact technical support with the CAS and SKU. Upon receipt, confirm identity by orthogonal techniques appropriate to small molecules (e.g., LC–MS, 1H/13C NMR, HRMS) as permitted by your internal QC workflow.

Synthetic Utility

Item-specific information

• The structure and functional groups of L-369 are not disclosed in the Product Data; therefore, no specific synthetic transformations can be recommended or described for this item.

General remarks for library compounds (non-product-specific)

• Many screening compounds feature heteroaromatic cores, amide/urea linkages, or halogenated motifs useful for late-stage diversification. If structural information becomes available, standard retrosynthetic disconnections (e.g., cross-coupling for aryl–aryl/aryl–vinyl, amide couplings, SNAr on activated heteroaryl systems) may be applicable.
• If derivatization is desired for SAR, confirm the presence of handles (e.g., aryl halides, boronates, amines, carboxylates) and assess compatibility with the planned conditions (Pd-catalyzed couplings, carbodiimide or HATU-mediated amide formation, etc.).

Recommendation

• Obtain the CoA/Spec Sheet or contact support for structural details before planning any synthetic use. Without known functionality, treat this material as a final compound rather than a building block.

Target Specificity

Item-specific data

• No biological target, binding partner, or specificity information is provided for L-369 in the Product Data.

Guidance (general)

• If this compound is used in screening and a putative target emerges, confirm specificity via orthogonal biochemical assays, counter-screens against related targets, and concentration–response analysis. Assess off-target profiles early to de-risk assay artifacts (e.g., aggregation, redox cycling, PAINS behavior) using appropriate controls.

Note

• Report any internally determined target associations alongside assay conditions and data quality metrics (e.g., Z′-factor, Hill slope) for reproducibility.

Perguntas frequentes

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1443522-24-4, the molecular formula is C49H95NO6, and the molecular weight is 794.28 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.