MAb de ratinho LAT2 - Anticorpo primário, specific to LAT2, Mouse IgG1

Cat. No.: Ab112832

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, WB

Host species

Rato

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

K52022_7A12

Conjugation

Unconjugated

Visão geral

O anticorpo anti-LAT2 humano de ratinho, monoclonal (K52022_7A12), pode ser utilizado para WB, IHC e assim por diante.
Aplicação:
WB: 1/1000
IHC: 1/100-1/200
Função da proteína
Envolvida na sinalização mediada por FCER1 (recetor epsilon de imunoglobulina de alta afinidade) em mastócitos. Pode também estar envolvida na sinalização mediada por BCR (recetor de antigénio de células B) nas células B e na sinalização mediada por FCGR1 (recetor Fc I de imunoglobulina gama de alta afinidade) nas células mieloides. Associa a ativação destes receptores e das suas cinases associadas a eventos intracelulares distais através do recrutamento de GRB2.
Pós-translacional
Fosforilado em tirosinas após a ligação cruzada de BCR nas células B, FCGR1 nas células mielóides ou FCER1 nos mastócitos, o que induz o recrutamento de GRB2.
Pode ser poliubiquitinado

Specifications

Product Name

MAb de ratinho LAT2 - Anticorpo primário, specific to LAT2, Mouse IgG1

Sinónimos

LAB anticorpo | LAT2 anticorpo | Linker para ativação de células B anticorpo | Linker para ativação de células B anticorpo | Linker para ativação de células T membro da família 2 anticorpo | Linker para ativação de células T adaptador transmembrana 2 anti

Especificações e pureza

ExactAb™, validado, sem suporte, 1,0 mg/mL

Espécie hospedeira

Rato

Especificidade

LAT2

Imunogénio

Recombinant protein of human LAT2 (AA 121-220).

Controlo positivo

WB: U937, Raji and THP-1 cell lysates. IHC: Human tonsil tissue.

Conjugação

Unconjugated

Grau

Carrier Free, ExactAb™, Validated

Isótipo

Mouse IgG1

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Concentração

1.0 mg/mL

Armazenamento e envio

Forma

Líquido

Condições de armazenamento de armazenamento

Conservar a -80°C. Evitar a congelação e descongelação repetidas

Enviado em

Dry ice packs + Cold packs

Estabilidade e armazenamento

Armazenar a 2-8 ℃ (3 meses). Armazenar a -20 ℃ (12 meses). Armazenar a -80 ℃ (36 meses). Após a receção, recomenda-se a aliquotagem. Evitar o ciclo de congelamento/descongelamento.

Imagens

Western Blot: LAT2 Mouse mAb (Ab112832)

LAT2 Mouse mAb (Ab112832) - Western Blot
All lanes: LAT2 Mouse mAb (Ab112832) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 27 kDa
Exposure time: 62.8 s

IHC: LAT2 Mouse mAb (Ab112832)

LAT2 Mouse mAb (Ab112832) - IHC
IHC analysis of tonsil tissue by LAT2 Mouse mAb (Ab112832).IHC was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with LAT2 Mouse mAb (Ab112832) at 7.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Germinal center cells and non-germinal center cells are positively stained at the cytoplasm and membrane. 

Western Blot: LAT2 Mouse mAb (Ab112832)

LAT2 Mouse mAb (Ab112832) - Western Blot
All lanes: LAT2 Mouse mAb (Ab112832) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 27 kDa
Exposure time: 100.0 s

Aplicação

• Aplicação: Dilution info
• IHC:

  1/100 - 1/200;

• WB:

  1/1000 ;

Alvos associados (humanos)

LAT2 Tbio Linker for activation of T-cells family member 2 (0 Activities)

Discover Target: LAT2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (LAT2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.