Mouse Copeptin (CPP) ELISA Kit

Cat. No.: EJ1515492

GRADE & PURITY BioReagent

Visão geral

This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Mouse CPP antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Mouse CPP in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.

Specifications

Especificações e pureza

BioReagent

Grau

BioReagent

Aplicação

ELISA

Tipo de amostra

homogeneizados de tecido, Lisado celular, Plasma, sobrenadantes de cultura celular, Soro

Gama de deteção

31.25pg/mL~2000pg/mL

Espécie detectada Objeto

Mouse

Especificidade

Detecting Mouse CPP in samples, with no significant cross-reactivity to other homologs.

Detection instrument

Microplate reader

Detection wavelength

450nm

Repeatability

The intra- and inter-assay coefficients of variation (CV) are both <10%.

Detection duration

3.5 hours, not including equilibration and sample preparation time.

Armazenamento e envio

Condições de armazenamento de armazenamento

Store at 2-8°C

Enviado em

Wet ice

Estabilidade e armazenamento

Store at 2-8℃ for 6 months.