mTOR inhibitor-8 - ≥98% , CAS No.2489196-70-3

CAS: 2489196-70-3 Cat. No.: M649812 Fórmula: C₂₄H₁₉ClN₄OS Peso molecular: 446.95

GRADE & PURITY ≥98%

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Visão geral

mTOR inhibitor-8 is an mTOR inhibitor and autophagy inducer. mTOR inhibitor-8 inhibits the activity of mTOR via FKBP12 and induces autophagy of A549 human lung cancer cells

In Vitro

mTOR inhibitor-8 (Compound 5e; 0.1-10 μM; 24 and 48 hours) suppresses the growth of A549 cells in a dose-dependent manner. mTOR inhibitor-8 induces autophagy in an mTOR-dependent manner. mTOR inhibitor-8 (10 μM; 3-24 hours) induces autophagy in a time-dependent manner. The levels of LC3B-II are enhanced. mTOR inhibitor-8 (10 μM; 3-24 hours) reduces the phosphorylation of RPS6KB1 (ribosomal protein S6 kinase) and EIF4EBP1 (eukaryotic translation initiation factor 4E-binding protein 1), two essential substrates of mTORMCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: A549 cells Concentration: 0.1, 1, 5, 10 μM Incubation Time: 24 and 48 hours Result: Suppressed the growth of A549 cells with an IC 50 of 2.6±0.11 μM. Western Blot AnalysisCell Line: A549 cells Concentration: 10 μM Incubation Time: 3, 6, 12 and 24 hours Result: The levels of phosphorylation of RPS6KB1 and EIF4EBP1 were significantly decreased after treatment.

In Vivo

mTOR inhibitor-8 (25 and 50 μM; 6 days) effectively inhibits tumor growth in vivo without adverse effect on normal chick chorioallantoic membrane angiogenesis . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Fertile chicken eggs (7-9 days old) Dosage: 25 and 50 μM Administration: 6 days Result: Significant xenograft tumor remission was observed in eggs compared with the DMSO-treated eggs.

Form:Solid

IC50& Target:mTOR Autophagy

Specifications

Especificações e pureza

≥98%

Mecanismos bioquímicos e fisiológicos

mTOR inhibitor-8 is an mTOR inhibitor and autophagy inducer. mTOR inhibitor-8 inhibits the activity of mTOR via FKBP12 and induces autophagy of A549 human lung cancer cells.

Condições de armazenamento de armazenamento

Store at -20°C

Enviado em

Ice chest + Ice pads

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Pureza

≥98%

Nomes e identificadores

• Sorrisos canónicos: COC1=CC=C(C2CC(C3=CC=C(Cl)C=C3)=NN2C4=NC(C5=CC=NC=C5)=CS4)C=C1
• Peso molecular: 446.95

Propriedades químicas e físicas

• Solubilidade: DMSO : 25 mg/mL (55.93 mM; Need ultrasonic)