Vermelho Nilo - BioReagente, para análise de fluorescência, ≥95% (HPLC) , CAS No.7385-67-3

CAS: 7385-67-3 Cat. No.: N121291 Fórmula: C₂₀H₁₈N₂O₂ Peso molecular: 318.37 Beilstein Registry Number: 279110 Número CE: 230-966-0

GRADE & PURITY BioReagent, for Fluorescence analysis, ≥95%(HPLC)

Synonyms

9-(diethylamino)-5H-benzo[a]phenoxazin-5-one | 9-(diethylamino)benzo[a]phenoxazin-5-one | Nile Blue A Oxazone | 9-(Diethylamino)-5H-benzo(a)pheno

Storage

Protegido da luz, temperatura ambiente, carregado com árgon

Shipped In

Normal

Visão geral

O vermelho do Nilo é uma molécula não carregada, hidrofóbica e fotoestável que fluoresce fortemente a vermelho vivo em ambientes hidrofóbicos. Este corante lipofílico é normalmente utilizado para a deteção de gotículas lipídicas intracelulares por microscopia de fluorescência e citofluorometria de fluxo. O vermelho do Nilo tem demonstrado detetar a exposição ou formação de novas superfícies hidrofóbicas induzidas pela ligação de ligandos à calmodulina, oligomerização da melitina ou desdobramento da ovalbumina durante a desnaturação térmica precoce. Este produto tem sido utilizado para corar estruturas celulares, bem como para visualizar e localizar transportadores de fármacos coloidais. A fluorescência do Nile Red não é afetada entre pH 4,5 e 8,5 e pode ser utilizada em concentrações diluídas para investigar locais de proteínas hidrofóbicas.
Um corante lipofílico fotoestável com fluorescência vermelha brilhante

Specifications

Sinónimos

9-(diethylamino)-5H-benzo[a]phenoxazin-5-one | 9-(diethylamino)benzo[a]phenoxazin-5-one | Nile Blue A Oxazone | 9-(Diethylamino)-5H-benzo(a)pheno

Especificações e pureza

BioReagente, para análise de fluorescência, ≥95% (HPLC)

Mecanismos bioquímicos e fisiológicos

Substância fotoestável, lipofílica, que fluoresce fortemente em ambientes hidrofóbicos (ricos em lípidos), mas tem uma fluorescência mínima em meios aquosos.

Condições de armazenamento de armazenamento

Protegido da luz, temperatura ambiente, carregado com árgon

Enviado em

Normal

Grau

BioReagent, para análise por fluorescência

Nota

Sempre que possível, deve preparar e utilizar as soluções no mesmo dia. No entanto, se precisar de preparar soluções de reserva com antecedência, recomendamos que guarde a solução como alíquotas em frascos hermeticamente fechados a -20°C. Em geral, estas podem ser utilizadas durante um mês. Antes de utilizar, e antes de abrir o frasco para injectáveis, recomendamos que deixe o seu produto equilibrar à temperatura ambiente durante pelo menos 1 hora. Precisa de mais conselhos sobre solubilidade, utilização e manuseamento? Para mais informações, visite a nossa página de perguntas frequentes (FAQ).

Pureza

≥95%(HPLC)

Nomes e identificadores

Pubchem Sid

504753968

Pubchem Sid Url

https://pubchem.ncbi.nlm.nih.gov/substance/504753968

Sorrisos canónicos

CCN(CC)C1=CC2=C(C=C1)N=C3C4=CC=CC=C4C(=O)C=C3O2

IUPAC Name

9-(diethylamino)benzo[a]phenoxazin-5-one

InChIKey

VOFUROIFQGPCGE-UHFFFAOYSA-N

INCHI

1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3

SMILES isoméricas

CCN(CC)C1=CC2=C(C=C1)N=C3C4=CC=CC=C4C(=O)C=C3O2

WGK Alemanha

3

Peso molecular

318.37

Beilstein

279110

Reaxy-Rn

279110

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=279110&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Classe Benzoxazines
      • Subclass Phenoxazines

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Classe: Benzoxazines
• Subclass: Phenoxazines
• Intermediate Tree Nodes: Not available
• Direct Parent: Phenoxazines
• Alternative Parents: Naphthalenes Dialkylarylamines Heteroaromatic compounds Oxacyclic compounds Azacyclic compounds Organopnictogen compounds Organooxygen compounds Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Phenoxazine - Naphthalene - Tertiary aliphatic/aromatic amine - Dialkylarylamine - Benzenoid - Heteroaromatic compound - Tertiary amine - Oxacycle - Azacycle - Amine - Hydrocarbon derivative - Organic oxide - Organopnictogen compound - Organic oxygen compound - Organooxygen compound - Organonitrogen compound - Organic nitrogen compound - Aromatic heteropolycyclic compound
• Descrição: This compound belongs to the class of organic compounds known as phenoxazines. These are polycyclic aromatic compounds containing a phenoxazine moiety, which is a linear tricyclic system that consists of a two benzene rings joined by a 1,4-oxazine ring.
• External Descriptors: organic heterotetracyclic compound

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Alvos associados (humanos)

VDR Tclin Vitamin D receptor (26531 Activities)

Discover Target: VDR

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

POLB Tchem DNA polymerase beta (23632 Activities)

Discover Target: POLB

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

PIN1 Tchem Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (36611 Activities)

Discover Target: PIN1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

FEN1 Tchem Flap endonuclease 1 (12055 Activities)

Discover Target: FEN1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

POLI Tchem DNA polymerase iota (116820 Activities)

Discover Target: POLI

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

KDM4A Tchem Lysine-specific demethylase 4A (52245 Activities)

Discover Target: KDM4A

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

BRCA1 Tchem Breast cancer type 1 susceptibility protein (15908 Activities)

Discover Target: BRCA1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

EHMT2 Tchem Histone-lysine N-methyltransferase, H3 lysine-9 specific 3 (93046 Activities)

Discover Target: EHMT2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

GPR55 Tclin G-protein coupled receptor 55 (1594 Activities)

Discover Target: GPR55

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

NPC1 Tchem Niemann-Pick C1 protein (18985 Activities)

Discover Target: NPC1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

RAB9A Tbio Ras-related protein Rab-9A (22488 Activities)

Discover Target: RAB9A

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MBNL1 Tbio Muscleblind-like protein 1 (34431 Activities)

Discover Target: MBNL1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

microRNA 21 (64692 Activities)

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ATAD5 Tbio ATPase family AAA domain-containing protein 5 (122566 Activities)

Discover Target: ATAD5

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

APOBEC3F Tbio DNA dC->dU-editing enzyme APOBEC-3F (14861 Activities)

Discover Target: APOBEC3F

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MEN1 Tchem Menin/Histone-lysine N-methyltransferase MLL (48157 Activities)

Discover Target: MEN1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Alvos associados (não humanos)

Plasmodium falciparum (966862 Activities)

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Plasmodium berghei (192651 Activities)

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Cavia porcellus (23802 Activities)

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L6 (7924 Activities)

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Luciferin 4-monooxygenase (66902 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Nfe2l2 Nuclear factor erythroid 2-related factor 2 (214 Activities)

Discover Target: Nfe2l2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de ação

Propriedades químicas e físicas

• Solubilidade: Soluble in water (Partly miscible), and methanol (1 mg/ml).
• Sensibilidade: light & air sensitive
• Ponto de fusão (°C): 203-205°C
• Peso molecular: 318.400 g/mol
• XLogP3: 3.800
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 3
• Exact Mass: 318.137 Da
• Monoisotopic Mass: 318.137 Da
• Topological Polar Surface Area: 41.900 Ų
• Heavy Atom Count: 24
• Formal Charge: 0
• Complexity: 564.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1