NQO1 Mouse mAb - Anticorpo primário, specific to Human NQO1

Cat. No.: Ab224769
Disponível para encomenda
GRAU & PUREZA Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 0.5 mg/mL
Application
ELISA, Flow Cytometry, WB
Host species
Rato
Reactive species
Humano
Isotype
Mouse IgG2b
Clone Number
AB03/1H11
Conjugation
Unconjugated
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Tamanho
USA
Alemanha (EU)*
Preço
Qtd
10μl
Ab224769-10μl
Fabricado sob encomenda nos EUA · 2–4 semanas ·
—
99,90US$
50μl
Ab224769-50μl
Fabricado sob encomenda nos EUA · 2–4 semanas ·
—
259,90US$
100μl
Ab224769-100μl
Fabricado sob encomenda nos EUA · 2–4 semanas ·
—
419,90US$
1ml
Ab224769-1ml
Fabricado sob encomenda nos EUA · 2–4 semanas ·
—
3399,90US$
Enter a quantity for the sizes you want to add.
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Por que este grau

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),0.5 mg/mL Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated para fluxos de trabalho cromatográficos e analíticos sensíveis que exigem interferência mínima da linha de base.

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Armazenamento e envio

Store at -20°C,Avoid repeated freezing and thawing Envio Ice chest + Ice pads. Consulte o COA específico do lote para obter as especificações exatas.

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Documentos de qualidade

SDS, COA, ficha técnica e folha de especificações disponíveis para download. COA específico do lote acessível por meio de pesquisa por número de lote.

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Comprovação documental

Citado em 0 publicações revisadas por pares nas áreas de cromatografia, síntese orgânica e reações de acoplamento cruzado.

Visão geral

Mouse anti Human NQO1 Antibody, clone AB03/1H11, could be used for WB, Flow Cytometry, ELISA and so on.

Especificações

Product Name
NQO1 Mouse mAb - Anticorpo primário, specific to Human NQO1
Sinónimos
Azoreductase antibody | Cytochrome b 5 reductase antibody | DHQU antibody | DIA 4 antibody | DIA4 antibody | Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody | Diaphorase (NADH/NADPH) antibody | Diaphorase 4 antibody | Dioxin inducible 1 antibo
Especificações e pureza
Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),0.5 mg/mL
Mecanismos bioquímicos e fisiológicos
Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofac
Espécie hospedeira
Rato
Especificidade
Human NQO1
Imunogénio
Recombinant full length protein corresponding human NQO1
Controlo positivo
WB: HepG2, HeLa, MCF-7 and A549 whole cell lysates Flow Cytometry: MCF-7 cells
Conjugação
Unconjugated
Grau
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Tipo de corrente de luz
kappa
SDS-PAGE
150.0 KDa
Método de purificação
Protein A purified
Concentração
0.5 mg/mL
Product Line
Produtos biológicos
Armazenamento e envio
Condições de armazenamento de armazenamento
Store at -20°C,Avoid repeated freezing and thawing
Enviado em
Ice chest + Ice pads
Estabilidade e armazenamento
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imagens

NQO1 Mouse mAb (Ab224769) - Western Blot
All lanes: NQO1 Mouse Antibody (Ab224769) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 31 kDa
Observed band size: 29 kDa
Exposure time: 30.0 s 

NQO1 Mouse mAb (Ab224769) - Flow Cytometry ​
Overlay histogram showing MCF-7 cells stained with NQO1 Mouse mAb (Ab224769) (Red) at 1.0 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue).​

Aplicação
Aplicação
Dilution info
WB

1/500-1/100000

Flow Cytometry

1/500

Documentação

📋 Ficha de dados de segurança (FDS)

Documento abrangente sobre perigos, manuseamento, armazenamento e conformidade regulamentar.

<<<<<<< HEAD Baixar SDS → ======= Baixar SDS → >>>>>>> release248-p3

✅ Certificado de análise (COA)

Dados de qualidade específicos do lote. Introduza o número de lote para obter o COA exato.

Consultar COA →

📊 Ficha técnica

Resumo de referência rápida das especificações e aplicações do produto.

Ver ficha técnica →

🔬 Folha de especificações

Atributos de qualidade completos e critérios de aceitação para este grau.

Ver folha de especificações →

Dados avançados

Alvos associados (humanos)
NQO1 Tchem NAD(P)H dehydrogenase [quinone] 1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificados(CoA,COO,BSE/TSE e Mapa de Análise)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Calculadoras de soluções
Revisões

Avaliações dos Clientes

Perguntas frequentes

How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
What is the purity of this product, and how is it determined?
This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (NQO1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.

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