PCNA Mouse mAb - Anticorpo primário, for WB,Flow,ELISA,ICC/IF various applications, from Human, generated in Mouse, specific to PCNA, IgG1

Cat. No.: Ab155826

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, See COA

Application

Flow Cytometry, IF/ICC, WB

Host species

Rato

Reactive species

Conselho, Humano, Rato

Isotype

Mouse IgG1

Clone Number

C04/3D7

Conjugation

Unconjugated

Visão geral

Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.

Specifications

Product Name

PCNA Mouse mAb - Anticorpo primário, for WB,Flow,ELISA,ICC/IF various applications, from Human, generated in Mouse, specific to PCNA, IgG1

Sinónimos

Cyclin | PCNA

Especificações e pureza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,See COA

Espécies hospedeiras

Mouse

Espécie hospedeira

Rato

Especificidade

PCNA

Aplicação

WB,Flow,ELISA,ICC/IF

Imunogénio

Full length recombinant human PCNA

Controlo positivo

WB: HeLa, A431, HEK293, HepG2, NIH3T3 and C6 whole cell lysates Flow: HeLa cells ICC/IF: HeLa cells

Conjugação

Unconjugated

Grau

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo de anticorpo

Primary antibody

Clonalidade

Monoclonal antibodies

Isótipo

IgG1

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Fonte

Hybridoma supernatant

Concentração

See COA

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze/thaw cycle.

Imagens

Western Blot: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution

Samples: Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

ICC/IF : PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - ICC/IF
IF analysis of PCNA (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (Ab155826) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Flow Cytometry
Flow analysis of PCNA (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. PCNA Mouse mAb (Ab155826) at 1/4000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.

Western Blot: PCNA Mouse mAb (Ab155826)

PCNA Mouse mAb (Ab155826) - Western Blot
All lanes: PCNA Antibody (Ab155826) at 0.05 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 29.0 kDa
Observed band size: 32.0 kDa
Exposure time: 97.0 s 

Aplicação

• Aplicação: Dilution info
• IF/ICC:

  0.5 μg/mL ;

• Flow Cytometry:

  0.016-2.0μg/mL ;

• WB:

  1/1,000-1/10,000 ; Predicted molecular weight: 29 kDa. ;

Alvos associados (humanos)

PCNA Tchem Proliferating cell nuclear antigen (0 Activities)

Discover Target: PCNA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação