Pea Extract Powder - BioReagent

Cat. No.: P1528486

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Visão geral

Pea extract powder is a plant-derived raw material for culture media, prepared from peas through extraction, filtration, drying, and other processes. It is rich in carbon sources, nitrogen sources, vitamins, and various growth factors required for microbial growth. In microbial cultivation, pea extract powder is commonly used as a basic nutritional additive, primarily functioning to provide carbon and nitrogen sources, vitamins, and growth factors for bacteria. It is also a common ingredient in the preparation of media such as blood agar and chocolate agar. Composition: Total nitrogen: 4%–5% Amino nitrogen: ≥ 2.0% Ash: < 35% Moisture: < 6.0% Phosphate precipitation: no precipitation Alkaline precipitation: no precipitation pH: 6.5–7.5

Specifications

Especificações e pureza

BioReagent

Condições de armazenamento de armazenamento

Room temperature

Enviado em

Normal

Grau

BioReagent

Product Line

biological_products

Application Protocols

No tested application protocols, assay conditions, or recommended dilutions are provided for this item in the Product Data.

• Item-specific protocols: Not specified for this item; refer to CoA/Spec Sheet and SDS.

Users should develop and validate protocols appropriate to their analytical goals (e.g., antioxidant assays, protein quantification, LC–MS profiling) and verify compatibility of solvents, pH, and additives with target methods.

Biological Roles

The extract derives from peas (Pisum sativum, literature), a legume with constituents spanning proteins, carbohydrates, lipids, and specialized metabolites. The following roles refer to classes of biomolecules commonly found in pea material; actual composition for this item is Not specified for this item; refer to CoA/Spec Sheet.

• Proteins and peptides: Pea seeds are rich in storage proteins (legumin [11S globulin] and vicilin [7S globulin]). These serve as nitrogen reserves in seeds. In research, they are models for studying protein structure, allergenicity, and digestibility.
• Carbohydrates: High starch content provides energy storage; dietary fibers (cellulose, hemicellulose, pectins) contribute to cell wall architecture. Oligosaccharides (e.g., raffinose family) are relevant in plant metabolism and can be profiled analytically.
• Phenolic compounds: Flavonoids and phenolic acids participate in plant defense and pigmentation and confer antioxidant properties measurable in cell-free assays.
• Saponins: Triterpenoid saponins are amphiphilic metabolites implicated in defense; they can influence membrane interactions in vitro and are studied for surfactant-like behavior.
• Minerals and trace elements: Essential cofactors for plant enzymes; levels vary with soil and processing.

Research utility:

• A pea extract matrix can be used to study enzyme kinetics (proteases, amylases), protein–polyphenol interactions, and antioxidant capacity. It also serves as a test matrix for method development in foodomics and metabolomics.

Note: All biological roles above are literature-based and may not reflect the exact composition of this product lot.

Buffer Applications

This product is not a buffering agent. However, it is often dispersed or extracted into buffers for biochemical analyses. No item-specific buffer recommendations are provided.

• Item-specific buffer guidance: Not specified for this item; refer to CoA/Spec Sheet.

General (literature/practice) considerations for preparing working suspensions/extracts:

• Choose neutral to mildly alkaline buffers (e.g., PBS, 10–50 mM Tris-HCl, pH 7.4–8.0) to enhance dispersion of proteinaceous fractions; adjust ionic strength to reduce aggregation.
• For phenolic assays or LC–MS, use volatile, LC-MS–compatible buffers at low ionic strength (e.g., 0.1% formic acid or ammonium formate) and pre-clear particulates.
• Avoid buffers containing components that interfere with the intended assay (e.g., Tris can interfere with certain colorimetric reagents; phosphate can chelate metals and impact catalysis).
• When required, add a small percentage of co-solvent (e.g., ethanol ≤20% v/v) to improve solubilization of phenolics; verify assay compatibility.

Preparation tips (general):

• Hydrate gradually under stirring; allow time for swelling of polysaccharides and protein hydration. Centrifuge and collect supernatant for analyses sensitive to turbidity.

For validated, item-specific buffer protocols, consult the product’s CoA or in-house method SOPs.

Green Alternatives

Pea Extract Powder is itself a bio-based material. Green-chemistry considerations center on how it is processed and used in the lab, particularly solvent choice and energy inputs.

Greener processing/usage strategies (general):

• Prefer aqueous and aqueous-ethanol systems over chlorinated or high-toxicity solvents for extraction and assay preparation.
• Use room-temperature or mild-heating extractions to minimize energy consumption; adopt ultrasound-assisted or stirred extraction to reduce time.
• Implement solid–liquid ratio optimization to reduce solvent volumes; recycle ethanol where feasible.
• For analytical cleanup, consider benign sorbents (cellulose, silica) and lower-toxicity elution solvents (ethanol, ethyl lactate) where compatible.

Illustrative comparison (general; not item-specific):

• Water or 50–70% ethanol: Low toxicity, renewable, suitable for proteins/phenolics; may require longer extraction times but offers safer handling and disposal.
• Methanol/acetone: Effective for some phenolics but higher toxicity and VOC emissions; prefer ethanol when performance allows.
• DMSO (minor co-solvent): High solvency; use sparingly and keep assay concentrations minimal to reduce environmental and biological impact.

Waste minimization:

• Clarify extracts via centrifugation/filtration to extend column life in chromatography.
• Scale microsample analytics (microplate assays, UHPLC) to reduce solvent use.

These strategies support greener workflows without compromising analytical rigor.

Pharmaceutical Uses

No pharmaceutical or clinical uses are claimed or supported for this product. It is supplied strictly for research use only (from Product Data).

Context from literature (not specific to this item; no claims):

• Components derived from peas (e.g., pea protein isolates, oligosaccharides, or phenolic-rich fractions) have been investigated in research settings as functional excipient candidates (e.g., matrix formers, stabilizers, or antioxidant constituents) in non-clinical formulation studies. Such explorations are experimental and subject to regulatory evaluation.

Practical notes for formulation research (general):

• Complex extracts are compositionally variable; if used in pre-formulation studies, establish a robust characterization panel (moisture, ash, protein/phenolic content, endotoxin/bioburden where relevant) and assess batch-to-batch consistency.
• Evaluate solubility/dispersibility in prospective formulation media and potential interactions (protein–polyphenol, protein–surfactant) that may affect stability or release profiles.

Regulatory status:

• No pharmacopeial monograph or excipient status is implied. Any use beyond basic research requires independent suitability assessments and compliance with applicable regulations.

For any application considerations beyond basic research, consult your quality unit and regulatory guidance.

Physical Properties

Item-specific physico-chemical specifications are not provided for this product.

• Form: Powder (from Product Name). Detailed appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Moisture content, ash, particle size, bulk density: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.
• pH (in solution), refractive index, optical rotation: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point/boiling point: Not applicable for complex mixtures of biopolymers.

General/literature guidance for pea-derived extracts (not item-specific):

• Many plant protein-rich powders are dispersible in water or buffers; true solubility depends on protein denaturation state, ionic strength, and pH. Phenolic- and saponin-rich fractions may show partial solubility in aqueous ethanol, methanol, or DMSO.
• Proteins exhibit pH-dependent solubility with minima near their isoelectric points (protein fraction from peas often shows reduced solubility around pH 4.5–5.5, literature). Adjustment to mildly alkaline pH (e.g., pH 7.5–8.5) can improve dispersion of proteinaceous components.
• Polyphenols commonly extract into 50–80% aqueous ethanol (literature). Starch/dietary fiber are largely water-insoluble but hydrate and swell.

Practical notes (general):

• For analytical work, pre-filter or centrifuge suspensions to remove insoluble fiber/starch prior to spectroscopic or chromatographic analysis.
• When preparing concentrated stocks of mixed extracts, consider a co-solvent system (e.g., aqueous buffer + small % ethanol or DMSO) to improve dissolution of hydrophobic constituents. Validate compatibility with downstream assays.

Quality and Grades

• Supplied grade: BioReagent (from Product Data).

What BioReagent typically implies (general industry usage; not a specification for this item):

• Suitable for biological research workflows where biomolecule integrity and low extraneous contaminants are important (e.g., enzymology, cell-free assays, biochemical analytics). It does not imply food-grade, clinical, or GMP status.
• Often accompanied by a basic characterization profile (e.g., moisture, ash, microbiological limits, or compositional markers), though the exact panel is vendor- and lot-dependent. For this item, specific QC attributes are Not specified for this item; refer to CoA/Spec Sheet.

Considerations specific to natural extracts:

• Composition can vary with botanical source, harvest, and extraction process. Researchers should verify that the lot’s profile (e.g., protein/phenolic content) meets experimental needs.
• Where downstream analytical quantitation is critical (antioxidant capacity, total phenolics, peptide mapping), request or establish a reference fingerprint (HPLC/LC–MS or FTIR).

Typical documents and tests to review (recommendations):

• CoA/Spec Sheet: for lot-specific composition metrics, appearance, moisture, microbial limits.
• SDS: for safety classification and handling.
• If needed for bioassays, consider in-house testing: endotoxin (LAL), bioburden, heavy metals (ICP-MS), or residual solvent GC. Note: Not specified for this item; confirm availability with supplier.

Reaction and Applications

This product is a biological extract rather than a discrete synthetic reagent; it is not typically used as a stoichiometric reactant or catalyst. However, it has several research applications as a source of natural constituents and as a complex biological matrix.

Research applications (general/literature):

• Antioxidant capacity studies: Determine total phenolic content (Folin–Ciocalteu), DPPH/ABTS radical scavenging, FRAP assays using ethanolic/water extracts.
• Protein/peptide analysis: Characterize pea proteins (legumin/vicilin) by SDS-PAGE, LC–MS/MS proteomics, amino acid analysis, digestibility assays.
• Carbohydrate profiling: Quantify starch and dietary fiber; employ enzymatic starch assays or HPAEC-PAD for oligosaccharides.
• Saponin/phenolic profiling: HPLC–DAD/ESI–MS for triterpenoid saponins and flavonoids; evaluate bioactivity in cell-free enzyme assays.
• Matrix effects: Use as a complex sample matrix when developing cleanup protocols (SPE, QuEChERS) or validating analytical methods for plant foods.

Practical notes:

• Pre-clear suspensions by centrifugation and filtration before spectroscopic or chromatographic measurements to minimize scattering and fouling.
• Standardize sample prep (solvent composition, extraction time, solid-to-liquid ratio) to ensure reproducibility.
• For quantitative comparisons across lots, normalize to dry mass and, if possible, to a marker analyte (e.g., total protein or total phenolics).

If specific functional applications are required, consult the CoA for composition markers and adjust protocols accordingly.

Reaction Conditions

Conventional “reaction conditions” in the sense of chemical synthesis are not typically applicable to a plant extract. Instead, researchers focus on extraction, dispersion, and analytical preparation. Item-specific conditions are not provided.

• Item-specific preparation parameters (solvent, temperature, time): Not specified for this item; refer to CoA/Spec Sheet.

General/literature guidance for preparing extracts/suspensions:

• Solid–liquid extraction: Use water, aqueous ethanol (30–80%), or methanol at room temperature to 50 °C with stirring or sonication for 10–60 min, adjusting solvent choice to the analyte class (proteins vs. phenolics vs. saponins). Avoid excessive heat to limit denaturation/oxidation.
• Clarification: Centrifuge (e.g., 5–15 min) and filter (0.45–0.22 μm) before chromatographic or spectrophotometric analysis.
• pH adjustment: For protein-rich fractions, maintain neutral to mildly alkaline pH to enhance solubility; for phenolic profiling, mildly acidic conditions (e.g., 0.1% formic acid) may stabilize analytes in LC–MS.
• Stabilization: Limit light/oxygen exposure during phenolic analysis; consider antioxidants (e.g., ascorbate) only if compatible with assays.

Expected outcomes (general):

• Turbid to clear supernatants depending on solvent and fraction. Yields and compositions vary widely with method and raw material variability.

For validated, reproducible procedures tailored to your endpoints, establish lab-specific SOPs and reference standards.

Safety and Handling

Hazard classification for this specific item is not provided.

• GHS classification, signal word, H-statements, pictograms: Not specified for this item; refer to SDS.
• Intended use: For research use only (from Product Data). Not for human or animal consumption, diagnostic, or therapeutic use.

General safety considerations for plant-derived powders (literature/practice):

• Potential hazards: Dust generation may cause mechanical irritation to the eyes/respiratory tract. Plant proteins and saponins can be allergenic/sensitizing in susceptible individuals.
• PPE: Use lab coat, safety glasses, and appropriate gloves. Employ dust control (handle in a fume hood or use local exhaust) when weighing.
• Incompatibilities: Avoid contact with strong oxidizers and strong acids/bases that can degrade biomolecules or generate heat. Prevent moisture ingress to avoid caking or microbial growth.
• First aid (general):
  • Inhalation: Move to fresh air. Seek medical advice if symptoms persist.
  • Skin contact: Wash with soap and water. Remove contaminated clothing.
  • Eye contact: Rinse cautiously with water for several minutes; remove contact lenses if present and easy to do; continue rinsing.
  • Ingestion: Rinse mouth. Do not induce vomiting; seek medical advice.
• Fire safety: Organic powders may be combustible. In case of fire, use water spray, CO2, dry chemical, or foam. Minimize dust clouds and ignition sources.

Always consult the product’s SDS for authoritative, lot-specific safety and regulatory information.

Solvent Selection

As a complex plant extract, optimal solvent choice depends on the target component class and the downstream assay. No item-specific solubility is provided for this product; guidance below is general/literature-based.

• Polarity and matrix: Mixed matrix containing proteins/peptides (polar, amphiphilic), polysaccharides (highly polar), phenolics/saponins (moderately polar), and minor lipophilic species.

Common solvent systems (general):

• Aqueous buffers (pH 6.5–8.5): Good for dispersing proteinaceous fractions; adjust ionic strength/pH to improve colloidal stability. Add 0.01–0.1% nonionic surfactant if needed (verify assay compatibility).
• Aqueous ethanol (30–80%): Enhances extraction/solubilization of phenolics and saponins; widely used in antioxidant assays.
• DMSO or DMF (small % as co-solvent): Useful to dissolve hydrophobic phenolics/saponins; keep final assay DMSO ≤1–2% to limit interference (literature practice).
• PBS or Tris buffers: Convenient for biochemical work; avoid phosphate if subsequent metal-catalyzed reactions are planned.

Selection tips:

• For spectrophotometric phenolic assays (e.g., Folin–Ciocalteu), use aqueous ethanol or methanol to maximize phenolic recovery, then dilute into assay buffer.
• For protein-focused analyses (SDS-PAGE, Bradford/BCA), prepare a buffered aqueous suspension, clear by centrifugation, and work with the supernatant.
• For LC–MS profiling, choose LC-MS–compatible solvents (water/acetonitrile with formic acid or ammonium formate) and remove particulates by 0.22–0.45 μm filtration.

Always verify solvent and pH compatibility with your specific assay.

Storage and Reconstitution

• Storage conditions: Room temperature (from Product Data). Protect from moisture and excessive heat. Keep container tightly closed.
• Shipping: Normal (from Product Data).
• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Reconstitution guidance: Not specified for this item; refer to CoA/Spec Sheet.

General handling notes (literature/practice; not item-specific):

• Store in a dry environment with desiccant to prevent caking and microbial growth. Avoid repeated opening in humid air.
• If preparing a stock suspension or extract, record solid mass, solvent composition, pH, and time; mix thoroughly and, if needed, clarify by centrifugation/filtration before use. Prepare fresh as needed to minimize oxidation of phenolics and degradation of proteins.
• For longer-term stability of prepared solutions, aliquot and store at 2–8 °C (short term) or −20 °C (longer term) as appropriate for the solvent system; verify stability for your assay. Avoid repeated freeze–thaw of protein-containing solutions by aliquoting.

Always consult the product’s CoA and SDS for authoritative storage and handling instructions specific to the supplied lot.

Structure and Identity

Pea Extract Powder (SKU: P1528486) is a complex, undefined mixture derived from plant material (typically Pisum sativum L., peas). As a natural extract, it does not correspond to a single molecular structure.

• Product type: Natural plant extract (powder); complex mixture of biomolecules
• Source organism (literature): Pisum sativum L. (garden pea). Specific source and part used for this item: Not specified for this item; refer to CoA/Spec Sheet.
• CAS, InChIKey, SMILES: Not applicable to complex mixtures; Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula and molecular weight: Not applicable for mixtures; Not specified for this item; refer to CoA/Spec Sheet.
• Grade/Purity: BioReagent (from Product Data)
• Appearance: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general, literature):

• Typical constituents reported for pea extracts include proteins/peptides (e.g., legumin/vicilin families), polysaccharides (starch, dietary fiber), phenolic compounds (flavonoids, phenolic acids), saponins, amino acids, lipids, and minerals. Exact composition is process- and lot-dependent.
• As a mixture, representation is best described by component classes rather than a single 2D structure. Proteins contain peptide backbones; phenolics possess aromatic rings with hydroxyl substituents; saponins contain triterpenoid/steroidal aglycones glycosylated with sugars.

Identity confirmation (general approaches):

• Lot-specific characterization typically employs protein content assays (Kjeldahl/Dumas), total phenolics (Folin–Ciocalteu, literature), HPLC/LC–MS fingerprints, and FTIR. Users should consult the CoA for actual identity/quality attributes of the supplied lot.

Synthetic Utility

As a complex natural extract, Pea Extract Powder is not a conventional building block for small-molecule synthesis. It has limited direct use in synthetic organic transformations. Nevertheless, it can contribute to chemical research in the following ways (general/literature):

• Source of natural analytes: Provides a matrix of amino acids, peptides, and phenolics for isolation/purification and subsequent structural elucidation (NMR, MS, HRMS). Purified components can serve as reference standards or substrates in enzyme-catalyzed reactions.
• Biocatalysis substrates: Proteinaceous content and endogenous enzymes (if present in minimally processed materials) can be used in exploratory protease, amylase, or glycosidase assays, though controlled addition of purified enzymes is preferred.
• Materials chemistry: Polysaccharide and protein fractions can be processed into films/gels as model bio-derived materials for studying crosslinking or surface modification chemistry.
• Analytical method development: Acts as a challenging matrix to validate extraction/cleanup (e.g., SPE, QuEChERS) and derivatization strategies for GC/LC analytics of bioactives.

Limitations:

• Undefined composition complicates stoichiometric chemistry; for reproducible synthesis, isolate and characterize target components first.
• Matrix effects may hinder catalysts or derivatization reagents; pre-fractionation (protein precipitation, solvent partitioning) is often required.

Item-specific reactivity, functional group content, and purity thresholds are Not specified for this item; refer to CoA/Spec Sheet.

Target Specificity

Not applicable. This product is not an antibody, protein capture reagent, or targeted binding agent.

• Antigen/epitope, species reactivity, clone, isotype: Not applicable and not specified for this item.

For products with defined biological targets (e.g., antibodies, enzymes), consult their dedicated listings.