Puromycin Hydrochloride Solution (10 mg/ml) - BioReagent,for cell culture,10 mg/ml , CAS No.58-58-2

CAS: 58-58-2 Cat. No.: P1509574 Fórmula: C₂₂H₂₉N₇O₅·2HCl Peso molecular: 544.44 Beilstein Registry Number: 3853613 Número CE: 200-387-8

GRADE & PURITY BioReagent, for Cell culture, 10 mg/ml

Synonyms

Puromycin Hydrochloride Solution

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Culture

Visão geral

  Puromycin (PM) is a protein synthesis inhibitor with a CAS number of 53-79-2. It has a structure similar to the terminal end of tRNA molecules, which enables it to bind to amino acids, replace aminoacylated tRNA to bind to the A-site of ribosomes, and be incorporated into the growing peptide chain. Although puromycin can bind to the A-site, it cannot participate in any subsequent reactions, thus terminating protein synthesis and releasing immature polypeptides containing puromycin at the C-terminus. Puromycin dihydrochloride is an aminonucleoside antibiotic produced by the fermentation of Streptomyces alboniger. As a stable hydrochloride form of puromycin, it is often referred to as puromycin hydrochloride, with a CAS number of 58-58-2.

  Puromycin Hydrochloride Solution (10 mg/mL) is formulated with high-purity raw materials and methanol. If necessary, it should be filtered through an organic filter membrane before being used for cell culture. Its molar concentration is 18.4 mmol/L, and the general working concentration ranges from 10 to 100 μmol/L. This product is for research use only (RUO) and is not intended for clinical diagnosis or any other applications.

Operating Procedures (For Reference Only)

1. Operate in accordance with specific experimental requirements.

2. Taking a working concentration of 100 μmol/L as an example, add 54.4 μL of Puromycin Solution (10 mg/mL) to every 10 mL of culture medium.

Precautions

1. Ensure sterile operation to avoid contamination. Minimize repeated freeze-thaw cycles to prevent reagent inactivation.

2. For your safety and health, wear a lab coat and disposable gloves during operation.

Specifications

Sinónimos

Puromycin Hydrochloride Solution

Especificações e pureza

BioReagent,for cell culture,10 mg/ml

Estabilidade e armazenamento

Store at -20℃ long term (12 months). Store in the dark.

Condições de armazenamento de armazenamento

Protected from light,Store at -20°C

Enviado em

Ice chest + Ice pads

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Grau

BioReagent, para cultura celular

Nomes e identificadores

• SMILES isoméricas: CN(C)C1=NC=NC2=C1N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl
• WGK Alemanha: 3
• RTECS: AU7355000
• CAS alternativo: 53-79-2
• Peso molecular: 544.44
• Beilstein: 3853613
• Reaxy-Rn: 39769009
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=39769009&ln=

Propriedades químicas e físicas

• Sensibilidade: Light-sensitive
• Ponto de fusão (°C): 174°C(lit.)

Referências

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3. Zhuozhou Hu, Jing Zhou, Liang Han, Xiangxiang Li, Chun Li, Tongyu Wu, Jingjing Liu, Wenyang Zhao, Jia Kang, Xinping Chen.  (2022)  Acyclovir alleviates insulin resistance via activating PKM1 in diabetic mice.  LIFE SCIENCES,      [PMID:35751919] [10.1016/j.lfs.2022.120725]

4. Qiong Yang, Maochen Xing, Ke Wang, Qiang Wei, Jiarui Zhao, Yuan Wang, Kai Ji, Shuliang Song.  (2022)  Application of Fucoidan in Caco-2 Model Establishment.  Pharmaceuticals,  15  (4): (418).  [PMID:35455415] [10.3390/ph15040418]

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6. Jia Liu, Liuting Zheng, Xinyue Li, Wei Tang, Manyu Guo, Yuxing Wang, Xiaoqi Tan, Jiajia Chang, Huiyue Zhao, Dongsheng Zhu, Yu-qiang Ma, Da Huo.  (2025)  Emerging of Ultrafine Membraneless Organelles as the Missing Piece of Nanostress: Mechanism of Biogenesis and Implications at Multilevels.  ACS Nano,      [PMID:39882824] [10.1021/acsnano.4c15876]

7. Zikang Xing, Xuewen Li, Zhen Ning Tony He, Xin Fang, Heng Liang, Chunxiang Kuang, Aiying Li, Qing Yang.  (2024)  IDO1 Inhibitor RY103 Suppresses Trp-GCN2-Mediated Angiogenesis and Counters Immunosuppression in Glioblastoma.  Pharmaceutics,  16  (7): (870).  [PMID:39065567] [10.3390/pharmaceutics16070870]

8. Ping Yang, Zheng-Jian Wang, Hai-Tao Lu, Xu-Meng Feng, Jing-Long Ye, Guangchuan Wang, Cheng-Feng Qin, Qing Ye, Zhong-Yu Liu.  (2024)  Imaging of viral replication in live cells by using split fluorescent protein-tagged reporter flaviviruses.  VIROLOGY,      [PMID:39754862] [10.1016/j.virol.2024.110374]

9. Xinrui Meng, Jingjing Liu, Jia Kang, Menghan Wang, Zhanghui Guan, Dong Tian, Xinping Chen.  (2024)  Lamivudine protects mice from gastric ulcer by activating PGK1 to suppress ferroptosis.  BIOCHEMICAL PHARMACOLOGY,      [PMID:39029631] [10.1016/j.bcp.2024.116440]

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Perguntas frequentes

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 58-58-2.

What does for cell culture mean?

for cell culture indicates that the product has been processed and tested for cell culture and tissue engineering. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.