MAb de ratinho RCAS1 - Anticorpo primário, specific to RCAS1, Mouse IgG1

Cat. No.: Ab124859

GRAU & PUREZA Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, WB

Host species

Rato

Reactive species

Conselho, Humano, Rato

Isotype

Mouse IgG1

Clone Number

K40051_4H7

Conjugation

Unconjugated

Visão geral

O anticorpo anti-RCAS1 humano de ratinho, monoclonal (K40051_4H7), pode ser utilizado para WB, IHC e assim por diante.
Aplicação:
WB:1/1000
IHC:1/100
Função da proteína:
Pode participar na supressão da proliferação celular e induzir a morte celular apoptótica através da ativação de proteases do tipo enzima conversora de interleucina-1-beta (ICE).

Especificações

Product Name

MAb de ratinho RCAS1 - Anticorpo primário, specific to RCAS1, Mouse IgG1

Sinónimos

Anticorpo PDAF | Anticorpo RCAS 1 | Anticorpo RCAS1 | Anticorpo RCAS1_HUMAN | Antigénio de ligação ao recetor do cancro expresso em células SiSo | Antigénio de ligação ao recetor do cancro expresso em células SiSo | EBAG9 | EB9 | PDAF | Anticorpo BAG9 | A

Especificações e pureza

ExactAb™, validado, sem suporte, 1,0 mg/mL

Espécie hospedeira

Rato

Especificidade

RCAS1

Imunogénio

Recombinant human EBAG9 (AA 41-210).

Controlo positivo

WB: SK-OV-3, MCF7, A549, Jurkat, HEK293, A431, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue.

Conjugação

Unconjugated

Grau

Carrier Free, ExactAb™, Validated

Isótipo

Mouse IgG1

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Concentração

1.0 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Líquido

Condições de armazenamento de armazenamento

Conservar a -80°C. Evitar a congelação e descongelação repetidas

Enviado em

Dry ice packs + Cold packs

Estabilidade e armazenamento

Armazenar a 2-8 ℃ (3 meses). Armazenar a -20 ℃ (12 meses). Armazenar a -80 ℃ (36 meses). Após a receção, recomenda-se a aliquotagem. Evitar o ciclo de congelamento/descongelamento.

Imagens

Western Blot: RCAS1 Mouse mAb (Ab124859)

RCAS1 Mouse mAb (Ab124859) - Western Blot
All lanes: RCAS1 Mouse mAb (Ab124859) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 31 kDa
Exposure time: 36.5 s

IHC: RCAS1 Mouse mAb (Ab124859)

RCAS1 Mouse mAb (Ab124859) - IHC
IHC analysis of human brain tissue by RCAS1 Mouse mAb (Ab124859). IHC was performed using sections of the formalin-fixed paraffin-embedded human brain tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with RCAS1 Mouse mAb (Ab124859) at 10 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Glial cells and neuronal cells are positively stained at the cytoplasm.

Aplicação

Aplicação

Dilution info

WB

1/1000

IHC

1/100

Alvos associados (humanos)

EBAG9 Tbio Receptor-binding cancer antigen expressed on SiSo cells (0 Activities)

Discover Target: EBAG9

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RCAS1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.