Recombinant Cdx4 Antibody - Anticorpo primário, specific to CDX4

Cat. No.: Ab095904

GRADE & PURITY Recombinant, ExactAb™, Validated, 0.8 mg/mL

Application

WB

Host species

Coelho

Reactive species

Conselho, Humano, Rato

Isotype

Rabbit IgG

Conjugation

Unconjugated

Visão geral

Rabbit anti Human Cdx4 Antibody, Recombinant, could be used for WB and so on.

Specifications

Product Name

Recombinant Cdx4 Antibody - Anticorpo primário, specific to CDX4

Sinónimos

Caudal-type homeobox protein 4 antibody | CDX4 antibody | CDX4_HUMAN antibody | Homeobox protein CDX-4 antibody | CDX4

Especificações e pureza

Recombinant, ExactAb™, Validated, 0.8 mg/mL

Mecanismos bioquímicos e fisiológicos

This gene encodes a member of a small subfamily of homeobox containing transcription factors. The encoded protein may regulate homeobox gene expression during anteroposterior patterning and hematopoiesis.

Espécie hospedeira

Coelho

Especificidade

CDX4

Imunogénio

A synthetic peptide derived from human Cdx4

Controlo positivo

WB: Hep G2, HeLa, RAW 264.7 and C6 cell lysates.

Conjugação

Unconjugated

Grau

ExactAb™, Recombinant, Validated

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Concentração

0.8 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: Recombinant Cdx4 Antibody (Ab095904)

Recombinant Cdx4 Antibody (Ab095904) - Western Blot
All lanes: Recombinant Cdx4 Antibody (Ab095904) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution

Predicted band size: 31 kDa
Observed band size: 43 kDa
Exposure time: 2.8 s

Western Blot: Recombinant Cdx4 Antibody (Ab095904)

Recombinant Cdx4 Antibody (Ab095904) - Western Blot
All lanes: Recombinant Cdx4 Antibody (Ab095904) at 1/1000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/5000 dilution

Predicted band size: 31 kDa
Observed band size: 43 kDa
Exposure time: 2.8 s

Aplicação

Aplicação

Dilution info

WB

1/1000

Alvos associados (humanos)

CDX4 Tbio Homeobox protein CDX-4 (0 Activities)

Discover Target: CDX4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Manufacturer-tested applications, recommended working dilutions, and positive control materials are not specified for this item.

General workflow reminders (not product-specific):

• Include appropriate positive and negative controls for each assay.
• Optimize antibody concentration via small-scale titration under your exact assay conditions.
• For transcription factor targets, consider nuclear extraction and stringent washing to enhance signal-to-noise.

Please consult the CoA/Spec Sheet for any item-specific protocols or validation data before first use.

Biological Roles

Cdx4 (caudal-type homeobox 4) is a homeodomain-containing transcription factor in the caudal-related CDX family. It plays pivotal roles in vertebrate embryonic development and lineage specification.

Key biological functions (literature):

• Posterior body patterning: Cdx4 integrates upstream signals (e.g., Wnt, FGF, retinoic acid) to regulate downstream gene programs controlling posterior axial elongation.
• Hox gene regulation: Cdx factors, including Cdx4, influence the Hox gene clusters, coordinating spatiotemporal expression that defines anterior–posterior identities.
• Hematopoietic development: In zebrafish and murine models, Cdx4 contributes to the emergence of definitive hematopoietic progenitors, partly via regulation of key hematopoietic transcriptional networks.
• Neurogenesis and mesodermal patterning: Cdx4 expression marks progenitor populations in the developing spinal cord and mesoderm, influencing differentiation trajectories.
• Chromatin interactions: As a sequence-specific DNA-binding protein, Cdx4 recruits co-regulators to enhancers/promoters, modulating chromatin accessibility and transcriptional output.

Cellular localization: Predominantly nuclear, consistent with its role as a transcription factor (literature).

Species context: Orthologs and conserved functions have been described in human, mouse, and zebrafish. Precise species reactivity of this specific antibody is not provided and should be verified in the product documentation.

Applications enabled: Anti-Cdx4 antibodies are used to map expression domains, assess developmental perturbations, and probe regulatory networks via WB, IF/IHC, IP, and ChIP (general, literature). No clinical/diagnostic claims are made.

Buffer Applications

This item is an antibody reagent rather than a buffering component. Traditional buffer formulation guidance (pKa, buffering ranges, recipes) is not applicable.

Practical note:

• Use standard immunoassay buffers (e.g., PBS or TBS, with/without mild detergents and protein carriers) as appropriate for your workflow, but defer to the item’s CoA/Spec Sheet for any required diluent or buffer composition. Item-specific buffer details are not specified.

Green Alternatives

While “green alternatives” typically concern organic solvents and reagents, a few sustainability considerations apply to antibody reagents:

• Cold-chain optimization: The product is shipped with ice pads (lower impact than dry ice). Consolidate orders to reduce packaging waste and transport emissions.
• Storage efficiency: Maintain -20°C storage; avoid unnecessary ultra-low temperature storage to reduce energy usage, provided performance is not compromised.
• Reusables: Employ reusable secondary containers and racks; recycle shipping materials where local programs allow.
• Minimizing waste: Aliquot to extend shelf life and reduce discard due to repeated freeze–thaw.
• Buffer choices (general): Prefer phosphate- or Tris-based buffers over high-toxicity preservatives where feasible; confirm that any changes remain compatible with the antibody and application.

Note: No formulation details (e.g., presence/absence of sodium azide or glycerol) are provided for this item. Any modification to buffers or preservatives should be validated experimentally to ensure that binding activity and specificity are maintained.

Pharmaceutical Uses

This product is for research use only. No therapeutic or clinical diagnostic use is claimed or supported.

Potential non-clinical roles (general):

• Process development and analytical characterization in biopharma research, such as target validation, pathway mapping, and assay development.
• Reference reagent in preclinical discovery platforms (e.g., immunoassays assessing Cdx4 expression in model systems).

Regulatory/compendial status: Not specified for this item; refer to CoA/Spec Sheet if applicable. No GMP/clinical-grade claims are made.

Physical Properties

Item-specific physicochemical parameters are not provided.

• Appearance/formulation: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration: Not specified for this item; refer to CoA/Spec Sheet.
• Buffer composition, pH, preservatives: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Antibodies are generally soluble in aqueous buffers (e.g., PBS, TBS) under near-neutral pH (literature, general). Item-specific solubility is not specified.
• Isoelectric point (pI): Antibodies typically span pI ~6–9 depending on sequence and glycosylation (literature, general). Not specified for this item.
• Extinction coefficient (A280): Sequence-dependent; not specified for this item.
• Stability considerations (general, literature):
  • Sensitive to freeze–thaw cycling; avoid repeated cycles (also specified for this item).
  • Stability often enhanced by carrier proteins (e.g., BSA) or cryoprotectants (e.g., glycerol) and storage at ≤−20°C; item-specific composition not specified.

Note: Without the item’s sequence, isotype, and buffer, precise properties (density, refractive index, logP, BP/MP) are not applicable/relevant to protein biologics. Please consult the CoA/Spec Sheet for item-specific details.

Quality and Grades

• Grade/Purity (product-specific): Recombinant, ExactAb™, Validated.
  • Recombinant antibodies are produced from defined expression constructs, providing lot-to-lot consistency, controlled sequence, and reduced background compared with traditional hybridoma sources (general).
  • ExactAb™: Proprietary brand designation indicating a recombinant format; typical benefits include defined sequence and reproducible performance. Specific platform details are not provided for this item.
  • Validated: Indicates that application-relevant performance has been assessed by the manufacturer; exact assays, species reactivity, and validation datasets are not specified for this item. Consult the CoA/Spec Sheet for details.

What this means in practice:

• Consistency: Recombinant production minimizes drift seen in long-maintained hybridomas (general).
• Purity: Recombinant preparations are commonly purified by protein A/G or affinity chromatographies; the exact method and residuals are not specified for this item.
• Stabilizers/preservatives: Not specified for this item; refer to CoA/Spec Sheet.

Fit-for-purpose guidance:

• For quantitative assays or longitudinal studies, recombinant format supports high reproducibility across lots (general).
• For sensitive detection (e.g., low-abundance nuclear transcription factors like Cdx4), validation data and application match (WB, IF, IHC, ChIP) should be confirmed in the item’s documentation before use.

Reaction and Applications

As an antibody reagent, this item is not used in synthetic organic reactions. Its value lies in selective binding to Cdx4 for analytical and discovery workflows.

Typical application areas for anti-Cdx4 antibodies (literature/general; not product-specific):

• Western blotting (WB): Detection of nuclear Cdx4 in embryonic or stem/progenitor cell lysates; consider nuclear extraction to enrich target.
• Immunofluorescence (IF) / Immunocytochemistry (ICC): Visualization of nuclear localization patterns; optimize fixation (e.g., PFA) and permeabilization.
• Immunohistochemistry (IHC): Tissue distribution studies in developing tissues; antigen retrieval conditions require empirical optimization.
• Chromatin immunoprecipitation (ChIP/qChIP-seq): Interrogation of Cdx4 binding at Hox clusters and developmental enhancers; requires high-specificity reagents and stringent wash conditions.
• Immunoprecipitation (IP): Enrichment of Cdx4 complexes for proteomics or DNA-binding assays.

Practical tips (general):

• Titrate antibody concentration for each application and sample type; use species-appropriate, minimal cross-reactivity secondary antibodies.
• For transcription factors, ensure appropriate nuclear extraction and include protease/phosphatase inhibitors.

Important: Tested applications, suggested dilutions, and species reactivity are not specified for this item and must be confirmed in the CoA/Spec Sheet or by in-lab validation.

Reaction Conditions

Classic reaction-condition parameters (solvent, catalysts, temperature, yields) are not applicable to antibody reagents.

Assay-oriented considerations (general, not product-specific):

• Incubation temperature/time for immunoassays (WB/IF/IHC/IP/ChIP) typically ranges from room temperature to 4°C with durations from 1 h to overnight, depending on assay and affinity.
• Buffers: PBS or TBS with appropriate blocking agents/detergents are common. Salt and detergent concentrations should be optimized empirically for specificity and background.

Tested conditions, recommended dilutions, and species reactivity are not specified for this item; consult the CoA/Spec Sheet or validate in-house.

Safety and Handling

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS.
• General risk profile: Protein antibodies are typically considered low hazard in standard laboratory use; however, treat as laboratory chemicals and follow institutional biosafety procedures.
• PPE: Use a lab coat, gloves, and safety glasses. Avoid inhalation/aerosol generation and contact with skin/eyes.
• Storage conditions (product-specific): Store at −20°C. Avoid repeated freezing and thawing.
• Shipping (product-specific): Shipped in an ice chest with ice pads to maintain the cold chain.
• Incompatibilities and stability risks (general):
  • Repeated freeze–thaw can denature antibodies and reduce binding activity.
  • Microbial contamination and proteases degrade proteins; use sterile technique.
  • Do not mix with oxidizing or denaturing agents unless protocol requires.
• First-aid overview (general): In case of skin/eye contact, rinse with water. If inhaled/ingested, seek medical attention according to institutional policy. Always consult the SDS for authoritative guidance.

Note: Buffer composition and any preservatives (e.g., sodium azide) are not specified for this item. Review the SDS/label for exact handling precautions.

Solvent Selection

This is a protein antibody reagent supplied for aqueous use. Organic solvent selection is generally not applicable.

General guidance (literature, not item-specific):

• Use physiological buffers such as PBS or TBS at near-neutral pH for dilution and handling, unless the product datasheet prescribes otherwise.
• Additives: Carrier proteins (e.g., BSA) and 0.01–0.05% nonionic detergents (e.g., Tween-20) are sometimes used to minimize adsorption and nonspecific binding; confirm compatibility with your assay.
• Cryoprotection: 10–50% glycerol is commonly used to maintain stability at −20°C; composition for this item is not specified.
• Avoid: Strong denaturants, high concentrations of organic solvents, or extreme pH unless required by a protocol (e.g., antigen retrieval is applied to tissue, not to the antibody stock).

Comparison (general):

• PBS vs TBS: TBS can reduce phosphate-sensitive interactions or precipitates in certain systems; PBS is widely used and compatible with most immunoassays.

Always defer to the item’s CoA/Spec Sheet for any provided buffer composition or required diluents. If not provided, validate buffer compatibility empirically with appropriate positive/negative controls.

Storage and Reconstitution

• Storage (product-specific): Store at −20°C. Avoid repeated freezing and thawing.
• Shipment (product-specific): Shipped in an ice chest with ice pads to maintain cold chain.
• Form/volume, buffer composition, preservatives, and concentration: Not specified for this item; refer to CoA/Spec Sheet.

Good practice (general, not item-specific):

• Upon receipt, briefly centrifuge to collect contents, inspect for container integrity, and record lot information.
• If frequent use is anticipated, prepare single-use aliquots to prevent freeze–thaw damage. Thaw on ice and mix gently; avoid vortexing.
• For long-term storage at −20°C, inclusion of glycerol as a cryoprotectant is common when compatible; verify against the item’s documentation.
• Do not store in frost-free freezers that cycle above freezing.

If lyophilized: Reconstitution buffer and volume are not specified for this item. Follow the CoA/Spec Sheet for precise instructions and allow the antibody to fully dissolve before use.

Structure and Identity

This product is a research-grade recombinant antibody directed against the transcription factor Cdx4 (caudal-type homeobox 4). As a protein-based biologic reagent, it does not have small-molecule identifiers.

• Product type: Recombinant antibody targeting Cdx4
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet. (Antibodies typically ~150 kDa for full-length IgG, literature.)
• SMILES: Not applicable to protein biologics.
• InChI / InChIKey: Not applicable to protein biologics.
• Structural features (general):
  • Typical IgG architecture comprises two heavy chains and two light chains stabilized by disulfide bonds (literature, general).
  • Antigen-binding fragments (Fabs) contain variable domains (VH/VL) that recognize epitopes within Cdx4; the Fc region mediates effector/handling properties (isotype not specified for this item).
  • Epitope, clone ID, species reactivity, and isotype: Not specified for this item; refer to CoA/Spec Sheet.

2D structure description (general): Y-shaped immunoglobulin with two antigen-binding paratopes at the tips of the Fab arms; the Fc stem forms the constant region. Exact sequence/epitope mapping for this item is not specified.

Synthetic Utility

Not applicable. As a protein antibody reagent, this product is not used as a building block or reagent in chemical synthesis.

For users seeking to study Cdx4-associated pathways, consult the Reaction & Applications and Biological Roles sections for guidance on analytical and discovery workflows (WB, IF/IHC, IP, ChIP; literature/general).

Target Specificity

• Target: Cdx4 (caudal-type homeobox 4)
• Clone/epitope: Not specified for this item; refer to CoA/Spec Sheet.
• Isotype/format/host: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity: Not specified for this item; refer to CoA/Spec Sheet.

Note: Provide positive control lysates/tissues known to express Cdx4 during validation. Item-specific details are required to determine compatibility with your samples and should be obtained from the CoA/Spec Sheet.

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Cdx4 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.