Recombinant E2F1 Antibody - Anticorpo primário, specific to E2F1, Rabbit IgG

Cat. No.: Ab100782

GRADE & PURITY Recombinant, Validated, ExactAb™, KD Validation, See COA

Application

IF/ICC, IHC, WB

Host species

Coelho

Reactive species

Conselho, Humano, Rato

Isotype

Rabbit IgG

Conjugation

Unconjugated

Visão geral

Rabbit anti Human E2F1 Antibody, Recombinant, could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

Recombinant E2F1 Antibody - Anticorpo primário, specific to E2F1, Rabbit IgG

Sinónimos

Dmel\CG6376 antibody | Dmel_CG6376 antibody | drosE2F1 antibody | E(Sev-CycE)3A antibody | E(var)3-93E antibody | E2-promoter binding facto antibody | E2F 1 antibody | E2F transcription factor 1 antibody | E2F-1 antibody | E2f-PA antibody | E2f-PB antibod

Especificações e pureza

Recombinant, ExactAb™, KD Validation, Validated, See COA

Mecanismos bioquímicos e fisiológicos

Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF

Espécie hospedeira

Coelho

Especificidade

E2F1

Imunogénio

A synthetic peptide derived from human E2F1 (AA 365-400)

Controlo positivo

WB: HeLa, A375, NIH/3T3 and C6 cell lysates. IHC: Human ovary carcinoma tissue. IF/ICC: HeLa cells.

Conjugação

Unconjugated

Grau

ExactAb™, KD Validation, Recombinant, Validated

Isótipo

Rabbit IgG

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Concentração

See COA

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: Recombinant E2F1 Antibody (Ab100782)

Recombinant E2F1 Antibody (Ab100782) - Western Blot
All lanes: Recombinant E2F1 Antibody (Ab100782) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 66, 74 kDa
Exposure time: 3.8 s

Western Blot: Recombinant E2F1 Antibody (Ab100782)

Recombinant E2F1 Antibody (Ab100782) - Western Blot
All lanes use Recombinant E2F1 Antibody (Ab100782) at 1/1000 dilution for 1 hour at room temperature.

IF/ICC: Recombinant E2F1 Antibody (Ab100782)

Recombinant E2F1 Antibody (Ab100782) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.

IF/ICC: Recombinant E2F1 Antibody (Ab100782)

Recombinant E2F1 Antibody (Ab100782) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782) at 1/50 dilution.

IF/ICC: Recombinant E2F1 Antibody (Ab100782)

Recombinant E2F1 Antibody (Ab100782) - IF/ICC
Immunofluorescent analysis of HeLa cells, using Recombinant E2F1 Antibody (Ab100782).

IHC: Recombinant E2F1 Antibody (Ab100782)

Recombinant E2F1 Antibody (Ab100782) - IHC
Immunohistochemical analysis of paraffin-embedded human ovary carcinoma, using Recombinant E2F1 Antibody (Ab100782).

Aplicação

• Aplicação: Dilution info
• IHC:

  1/100 - 1/200;

• WB:

  1/1000 - 1/2000

• IF/ICC:

  1/50 - 1/200;

Alvos associados (humanos)

E2F1 Tbio Transcription factor E2F1 (0 Activities)

Discover Target: E2F1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

E2F1 Tbio Transcription factor E2F1 (14 Activities)

Discover Target: E2F1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant E2F1 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.