GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.2 mg/mL
ExactAb™, Validated, Recombinant, 0.2 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Rabbit anti Human eIF4EBP1 Antibody, Recombinant, could be used for WB, IHC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Regulates eIF4E activity by preventing its assembly into the eIF4F complex. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Specifications
Product Name
Recombinant eIF4EBP1 Antibody - Primary antibody, specific to EIF4EBP1, Rabbit IgG
Synonyms
4EBP1 | 4E-BP1 | eIF4E-binding protein 1 | EIF4EBP1 | eukaryotic translation initiation factor 4E binding protein 1, 4EBP1, 4E-BP1BP-1 | eukaryotic translation initiation factor 4E-binding protein 1 | PHAS-I | PHAS-IMGC4316 | Phosphorylated heat- and acid-s
Specifications & Purity
ExactAb™, Validated, Recombinant, 0.2 mg/mL
Host species
Rabbit
Specificity
EIF4EBP1
Immunogen
A synthetic peptide derived from human eIF4EBP1 (AA 16-33)
Positive Control
WB: Jurkat, HEK293, Hep G2, HeLa, MCF7, A431, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Recombinant, Validated
Isotype
Rabbit IgG
SDS-PAGE
150 kDa
Purification Method
Antigen affinity purified
Concentration
0.2 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant eIF4EBP1 Antibody (Ab101403) - Western Blot All lanes: Recombinant eIF4EBP1 Antibody (Ab101403) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 13 kDa Observed band size: 18 kDa Exposure time: 78.9 s
Recombinant eIF4EBP1 Antibody (Ab101403) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant eIF4EBP1 Antibody (Ab101403). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant eIF4EBP1 Antibody (Ab101403) - IF/ICC IF analysis of Recombinant eIF4EBP1 Antibody (Ab101403) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant eIF4EBP1 Antibody (Ab101403) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Application
Application
Dilution info
WB
1/500-1/1000
IHC
1/50 - 1/100
IF/ICC
1/200
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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