GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.3 mg/mL
ExactAb™, Validated, Recombinant, 0.3 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Visão geral
Rabbit anti Human PUMA Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on. Application: WB: 1/500-1/1000 IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein Function Essential mediator of p53-dependent and p53-independent apoptosis.
Specifications
Product Name
Recombinant PUMA Antibody - Anticorpo primário, specific to BBC3, Rabbit IgG
A synthetic peptide derived from human PUMA (AA 177-193)
Controlo positivo
WB: Jurkat, THP-1, HeLa, K562, HEK293, C6 and NIH/3T3 cell lysates. IHC: Human breast cancer tissue. ICC/IF: 293T cells.
Conjugação
Unconjugated
Grau
ExactAb™, Recombinant, Validated
Isótipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificação
Immunogen affinity purified
Concentração
0.3 mg/mL
Armazenamento e envio
Forma
Liquid
Condições de armazenamento de armazenamento
Store at -20°C,Avoid repeated freezing and thawing
Enviado em
Ice chest + Ice pads
Estabilidade e armazenamento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imagens
Recombinant PUMA Antibody (Ab123948) - Western Blot All lanes: Recombinant PUMA Antibody (Ab123948) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 21 kDa Observed band size: 19, 25 kDa Exposure time: 8.0 s
Recombinant PUMA Antibody (Ab123948) - IF/ICC Immunocytochemistry analysis of PUMA (green) in 293T using Recombinant PUMA Antibody (Ab123948), and cells were counterstained with DAPI (blue).
Recombinant PUMA Antibody (Ab123948) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant PUMA Antibody (Ab123948). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Aplicação
Aplicação
Dilution info
WB
1/500-1/1000
IF/ICC
1/50-1/200
IHC
1/50 - 1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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