Recombinant Tissue Factor Antibody - Anticorpo primário, specific to F3, Rabbit IgG

Cat. No.: Ab131401

GRADE & PURITY Recombinant, ExactAb™, Validated, 0.3 mg/mL

Application

IHC, WB

Host species

Coelho

Reactive species

Conselho, Humano, Rato

Isotype

Rabbit IgG

Conjugation

Unconjugated

Visão geral

Rabbit anti Human Tissue Factor Antibody, Recombinant, could be used for WB, IHC and so on.
Application:
WB: 1/500-1/1000
IHC: 1/50-1/100
Protein Function
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or × by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Specifications

Product Name

Recombinant Tissue Factor Antibody - Anticorpo primário, specific to F3, Rabbit IgG

Sinónimos

CD142 antibody | CD142 antigen antibody | Coagulation factor III (thromboplastin tissue factor) antibody | Coagulation factor III antibody | F3 antibody | FLJ17960 antibody | TF antibody | TF_HUMAN antibody | TFA antibody | Thromboplastin antibody | F3 |

Especificações e pureza

ExactAb™, Validated, Recombinant, 0.3 mg/mL

Espécie hospedeira

Coelho

Especificidade

F3

Imunogénio

A synthetic peptide derived from human Tissue Factor (AA 39-52)

Controlo positivo

WB: Mouse brain, HeLa, A431, NIH/3T3 and C6 lysates. IHC: Human lung cancer tissue.

Conjugação

Unconjugated

Grau

ExactAb™, Recombinant, Validated

Isótipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificação

Antigen affinity purified

Concentração

0.3 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: Recombinant Tissue Factor Antibody (Ab131401)

Recombinant Tissue Factor Antibody (Ab131401) - Western Blot
All lanes: Recombinant Tissue Factor Antibody (Ab131401) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 45 kDa
Exposure time: 113.4 s

Western Blot: Recombinant Tissue Factor Antibody (Ab131401)

Recombinant Tissue Factor Antibody (Ab131401) - Western Blot
All lanes: Recombinant Tissue Factor Antibody (Ab131401) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 33 kDa
Observed band size: 45 kDa
Exposure time: 300.0 s

IHC: Recombinant Tissue Factor Antibody (Ab131401)

Recombinant Tissue Factor Antibody (Ab131401) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant Tissue Factor Antibody (Ab131401). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Aplicação

Aplicação

Dilution info

WB

1/500-1/1000

IHC

1/50 - 1/100

Alvos associados (humanos)

F3 Tclin Tissue factor (0 Activities)

Discover Target: F3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação

Application Protocols

Item-specific facts (from Product Data)

• Manufacturer Applications: Not specified for this item; refer to CoA/Spec Sheet.

General starting-point protocols (to be optimized; literature)

• Western blot: Start at 1:500–1:2000 primary dilution; incubate 1 h at room temp or overnight at 4°C. Use appropriate membrane blocking (5% BSA or milk) based on epitope sensitivity.
• IHC/IF: Begin with 1–10 µg/mL or 1:100–1:500 dilution. Optimize antigen retrieval (citrate pH 6 or Tris-EDTA pH 9) and fixation method.
• Flow cytometry: 0.1–1 µg per 10^6 cells as a starting range. Include Fc-block if using immune cells and appropriate viability dye.
• ELISA: 0.1–1 µg/mL for capture or detection, depending on format; perform checkerboard titration.

Controls and validation (general)

• Include isotype controls, secondary-only controls, and TF-positive/negative biological controls. Confirm linear dynamic range and limit of detection.

Note

• The above are general literature ranges and not specifications for this item. Use the CoA/Spec Sheet for any manufacturer-validated conditions.

Biological Roles

General biology of the target (literature)

• Tissue Factor (TF; gene F3, also known as CD142) is a transmembrane glycoprotein that functions as the primary initiator of the extrinsic coagulation pathway. TF binds Factor VII/VIIa, forming the TF–FVIIa complex that activates Factors IX and X, promoting thrombin generation and fibrin formation.
• Expression: Predominantly found in subendothelial cells such as smooth muscle cells and fibroblasts; exposure upon vascular injury triggers coagulation. TF expression can be inducible in various cell types under inflammatory or stress conditions.
• Structure: TF comprises an extracellular domain that binds FVII/FVIIa, a single-pass transmembrane helix, and a short cytoplasmic tail implicated in signaling.
• Research significance: TF is widely studied in hemostasis, thrombosis, inflammation-associated coagulation, and cell signaling contexts. Antibodies to TF are used to map expression patterns, quantify surface levels, and interrogate TF–FVIIa signaling.

Item-specific note

• This antibody is described as a “Recombinant Tissue Factor Antibody.” Specific epitope, isotype, affinity, species cross-reactivity, and validated applications for this item are not specified; refer to the CoA/Spec Sheet for definitive details.

Compliance

• For research use only. No medical or clinical diagnostic claims are made or implied.

Buffer Applications

Applicability

• This product is an antibody reagent, not a buffering agent. Therefore, buffer formulation as a primary application is not applicable.

Practical guidance for use (general)

• Working buffers: PBS or TBS at pH ~7.2–7.6 are commonly used for antibody dilution. Include blocking proteins (e.g., BSA) and mild detergents as your assay requires; exact compatibility for this item is not specified.
• Storage buffer: Composition for this item is not specified; consult the CoA/Spec Sheet. Avoid repeated freeze–thaw by aliquoting into low-binding tubes.
• pH sensitivity: Extreme pH can denature antibodies or alter binding; maintain near-neutral conditions unless performing controlled elution steps.

Note

• For validated buffers and any required additives specific to this antibody, refer to the CoA/Spec Sheet.

Green Alternatives

Context

• Antibody use typically involves aqueous buffers and minimal volumes of hazardous solvents. “Green” considerations focus on buffer composition, blocking agents, detection chemistries, and waste minimization.

Greener practice suggestions (general)

• Aqueous-only workflows: Favor PBS/TBS with biodegradable detergents at minimal effective concentrations. Avoid chlorinated solvents and minimize methanol or acetone use for fixation unless required by the protocol.
• Blocking and detergents: Use plant-derived or certified sustainable blocking agents when compatible. Select low-toxicity detergents and optimize to reduce volumes.
• Detection chemistry: Prefer non-heavy-metal fluorophores or enzyme substrates with lower hazard classifications; evaluate chemiluminescent reagents with improved EHS profiles.
• Waste reduction: Optimize antibody titers to reduce reagent consumption; employ mini-blot and micro-IHC formats; regenerate affinity matrices when possible.
• Cold-chain efficiency: Consolidate shipments and use reusable cold packs where allowable to reduce packaging footprint.

Item-specific note

• No special formulation details are provided for this item; assess preservative and buffer components from the CoA/SDS and adapt greener alternatives where compatible with performance.

Pharmaceutical Uses

Applicability

• This product is supplied for research use only. It is not intended for human or veterinary use, clinical diagnostics, or as an active pharmaceutical ingredient.

General context (literature/practice)

• In pharmaceutical R&D, research-grade antibodies can be employed as analytical reagents in assays such as ELISA, Western blot, flow cytometry, or immunocapture during process and product characterization. They may support biomarker quantitation and mechanistic studies in preclinical research.
• Reagent considerations: For GMP or regulated uses, purpose-built reagents with full qualification, traceability, and change control are required. Specifications such as isotype, sequence, endotoxin, bioburden, and stability must be established; these are not specified here.

Item-specific note

• No pharmacopeial status, excipient role, or GMP suitability is provided for this item. For any regulated application, request documentation and assess suitability under your quality system.

Physical Properties

Item-specific facts (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration, buffer, excipients, pH: Not specified for this item; refer to CoA/Spec Sheet.

General properties for antibodies (literature)

• Approximate size: IgG antibodies migrate near ~150 kDa by non-reducing SDS-PAGE; heavy chain ~50 kDa and light chain ~25 kDa by reducing SDS-PAGE. Actual values depend on isotype and glycosylation.
• Solubility: Readily soluble in aqueous buffers (e.g., PBS, TBS) at neutral pH; precipitation can occur with extreme pH, high salt, or denaturants.
• Isoelectric point (pI): Often in the 6–9 range for IgGs, clone-dependent.
• Stability: Antibodies are sensitive to repeated freeze–thaw; stability is enhanced by aliquoting and storage at recommended temperatures.
• Optical properties: Protein solutions typically show A280 for quantification (ε depends on sequence); UV–Vis profile will vary with formulation and concentration.

Notes

• Do not treat general literature values as specifications for this item. Where exact values are required for method validation or comparability, consult the product CoA/Spec Sheet.

Quality and Grades

Item-specific facts (from Product Data)

• Grade/Purity: Recombinant, ExactAb™, Validated.

Interpretation and implications (general)

• Recombinant: Produced via controlled expression systems (e.g., mammalian or other hosts) for enhanced lot-to-lot consistency versus hybridoma-derived or polyclonal antibodies. Benefits include defined sequence and streamlined scalability. Exact host system for this item is not specified.
• ExactAb™: Branded designation indicating a defined, sequence-controlled recombinant antibody offering enhanced reproducibility. It typically implies stringent release testing; refer to the CoA for the precise performance metrics tied to this item.
• Validated: Indicates the antibody has undergone application-oriented testing. The specific tested applications, species reactivity, and positive controls for this item are not specified; consult the CoA/Spec Sheet for exact validation scope and results.

What to check on receipt

• Review CoA for: isotype, concentration, buffer/preservatives, QC assays (purity by SDS-PAGE/SEC, endotoxin if relevant, binding specificity/affinity), and validated applications with recommended dilutions. Any such specifics are not provided here.
• Keep documentation for method qualification/transfer and to ensure reproducibility across lots.

Reaction and Applications

Item-specific facts (from Product Data)

• Labeled “Recombinant Tissue Factor Antibody”; validated, but specific tested applications are not provided. Use is for research only.

General research applications for Tissue Factor antibodies (literature)

• Immunodetection: Western blotting (WB) to detect TF expression in lysates; immunohistochemistry (IHC/IHC‑P/IHC‑F) for tissue localization; immunofluorescence (IF) for cellular localization; ELISA for quantitative measurement; flow cytometry (FC) for surface TF on cells. Exact suitability and dilutions for this item are not specified.
• Immunocapture: Pull-down/IP to enrich TF and co-complexes; validate epitope accessibility and denaturation sensitivity before use.
• Assay development: Reference controls, standard curves, and spike-recovery experiments for quantitative workflows. Include appropriate negative and isotype controls.

Practical tips (general)

• Antigen retrieval: For IHC, test citrate vs EDTA retrieval conditions; TF epitope exposure can be fixation-dependent.
• Controls: Use TF-positive cell lines/tissues and TF-knockdown/knockout as negative controls where feasible.
• Titration: Perform checkerboard titrations to determine optimal antibody concentration and incubation time; avoid high backgrounds by optimizing blocking and washing.
• Detection: Choose secondary antibodies compatible with the primary’s isotype and species; if this is a recombinant monoclonal, isotype must be confirmed from the CoA.

Note

• Application-specific recommendations for this item (dilutions, species reactivity, incubation times) are not specified; consult the CoA/Spec Sheet.

Reaction Conditions

Not typically applicable

• Classical reaction condition scouting (solvent, temperature, catalysts) does not apply to antibody products.

Assay condition guidance (general)

• Temperature: Perform binding/incubations at 4–25°C depending on assay type; avoid elevated temperatures that can denature antibodies.
• Incubation: Primary antibody incubations range from 1–2 h at room temperature to overnight at 4°C; actual optimal conditions must be empirically determined.
• Buffers: PBS or TBS with optimized blocking agents and detergents (e.g., 0.05–0.1% Tween-20 for WB/ELISA) are common. For flow cytometry, use protein-containing staining buffers to minimize non-specific binding.
• Washes: Thorough but gentle washing reduces background while preserving antigen integrity.

Item-specific note

• Validated application conditions (dilutions, times, retrieval, species) for this antibody are not specified; refer to the CoA/Spec Sheet and optimize for your system.

Safety and Handling

Item-specific facts (from Product Data)

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS.
• Storage conditions: Store at -20°C. Avoid repeated freezing and thawing.
• Shipping: Ice chest + ice pads (cold chain).

General guidance for antibodies and protein reagents (literature/practice)

• Hazard profile: Most purified antibodies in aqueous buffers are not classified as hazardous; however, always consult the SDS. Preservatives (e.g., sodium azide) may be present in some formulations; composition for this item is not specified.
• PPE: Laboratory coat, nitrile gloves, and safety eyewear. Avoid ingestion, inhalation of aerosols, and contact with skin/eyes.
• Handling: Keep on ice during use. Mix gently—avoid vigorous vortexing and foaming which can denature proteins. Use low-retention tips and protein-compatible plastics.
• Incompatibilities: Avoid strong oxidants, extreme pH, and proteases. Metal chelators, detergents, or carrier proteins may affect downstream assays depending on the formulation; composition for this item is not specified.
• First aid (overview): Skin/eye contact—rinse with water. Ingestion—rinse mouth. Inhalation—move to fresh air. Seek medical attention if symptoms persist. SDS provides authoritative guidance.
• Waste: Dispose of proteinaceous materials and any biologicals according to institutional biosafety procedures and local regulations.
• Freeze–thaw: Minimize cycles by preparing single-use aliquots upon first thaw.

Solvent Selection

Applicability

• This is an aqueous protein reagent; organic solvent selection is generally not applicable. Focus instead on compatible biological buffers and additives.

Practical guidance (general)

• Compatible buffers (literature): Phosphate-buffered saline (PBS, pH ~7.2–7.4) or Tris-buffered saline (TBS) are commonly used for dilution and storage. Additives such as carrier proteins or mild detergents can impact assays; composition for this item is not specified.
• Avoid: Strong organic solvents, extreme pH, chaotropes, and high shear, which can denature antibodies or disrupt binding.
• Filtration: Use low protein-binding membranes (e.g., PVDF with appropriate pore size) if sterile filtration is required.

Note

• For this specific item’s formulation and compatible diluents, details are not specified; refer to the CoA/Spec Sheet and validate buffer conditions in your assay system.

Storage and Reconstitution

Item-specific facts (from Product Data)

• Storage: Store at -20°C. Avoid repeated freezing and thawing.
• Shipping: Ice chest + ice pads.
• Appearance, buffer, concentration, preservative: Not specified for this item; refer to CoA/Spec Sheet.

General recommendations (literature/practice)

• Upon receipt: Verify integrity of cold chain. Centrifuge briefly to collect contents. Inspect for turbidity or precipitate.
• Aliquoting: Prepare single-use aliquots in low-binding tubes to minimize freeze–thaw cycles. Record aliquot concentration and date.
• Thawing/handling: Thaw on ice. Mix gently by pipetting; avoid vigorous vortexing or frothing.
• Working solutions: Dilute with sterile, protein-compatible buffer (e.g., PBS/TBS with carrier protein if appropriate for the assay). Filter sterilize only if necessary using low protein-binding membranes.
• Short-term storage: Keep on ice during use; store at 2–8°C for short periods if compatible with formulation. For long-term stability, adhere to the specified -20°C storage.
• Reconstitution: If supplied lyophilized (not specified), reconstitute with sterile water or buffer per CoA instructions; allow gentle rocking to dissolve completely.

Note

• Because formulation and concentration are not specified for this item, always consult the CoA/Spec Sheet for definitive guidance on reconstitution volumes, recommended diluents, and stability after opening.

Structure and Identity

Item-specific facts (from Product Data)

• Product: Recombinant Tissue Factor Antibody (SKU: Ab131401)
• Grade/Purity: Recombinant, ExactAb™, Validated
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES / InChIKey / CAS / CID: Not applicable to proteins; not specified for this item; refer to CoA/Spec Sheet.

General description (literature)

• Molecular class: Immunoglobulin (antibody) protein reagent, recombinant production.
• Typical architecture: Y‑shaped heterotetramer (2 heavy + 2 light chains) with variable regions that bind antigen and constant regions that mediate Fc functions. If IgG, an apparent MW is typically ~150 kDa; actual size depends on isotype and glycosylation.
• Target: Tissue Factor (TF), a cell-surface glycoprotein also known as coagulation factor III (F3/CD142). The antibody’s variable region is designed to recognize an epitope on TF; exact epitope/isotype are not specified for this item.

2D/functional depiction in words (literature)

• Two antigen-binding fragments (Fabs) at the arms with complementarity-determining regions (CDRs) that engage Tissue Factor epitopes; one Fc stem that may be used for secondary antibody recognition or conjugation depending on isotype. Exact primary sequence and post-translational modifications are not specified for this item.

Synthetic Utility

Not typically applicable

• As an antibody reagent, this product is not used as a synthetic building block or reagent in classical organic synthesis.

Where conceptually relevant (general)

• Conjugation chemistry: Antibodies can be conjugated to enzymes, fluorophores, biotin, or linkers using chemistries such as NHS-ester labeling (lysines), maleimide-thiol coupling (reduced cysteines), click chemistry (azide–alkyne), or site-specific methods (e.g., enzymatic tags). For this item, no conjugation state or recommendations are specified.
• Immobilization: Passive adsorption or covalent coupling (e.g., EDC/NHS to carboxylated matrices or Protein A/G capture) for immunoaffinity applications.

Item-specific note

• Any sequence, isotype, or formulation details required to plan site-specific modifications are not specified; consult the CoA/Spec Sheet before developing conjugation workflows.

Target Specificity

Item-specific facts (from Product Data)

• Antigen name: Tissue Factor
• Clone, isotype, host species, conjugate, epitope: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity and cross-reactivity: Not specified for this item; refer to CoA/Spec Sheet.
• Applications validated by manufacturer: Not specified for this item; refer to CoA/Spec Sheet.

Notes

• Only target name is inferred from the product name. For precise specificity (epitope, affinity, off-target assessment), consult the CoA/Spec Sheet and SDS.

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant Tissue Factor Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.