Red Blood Cell Lysis Buffer (10×) - sterile-filtered, BioReagent, for cell culture, 10x

Cat. No.: A1372415

GRADE & PURITY Sterile-filtered, BioReagent, for Cell culture, 10x

Synonyms

RBC Lysis Buffer | Lysis Buffer

Storage

Store at 2-8°C

Shipped In

Wet ice

Visão geral

Red Blood Cell Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution based ammonium chloride, and should be diluted in deionized water prior to use.

Instructions for Use：

Lysis of Human Peripheral Blood:

1. Dilute the 10X concentrate 1:10 with distilled water to prepare the 1x lysis buffer. Warm the 1X solution to room temperature prior to use.

2. Add 20 ml of 1X RBC Lysis Buffer to each tube containing up to 1mL of whole blood.

3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature for 10-15 minutes.

4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing pellet.

5. Resuspend the pellet in the appropriate buffer, e.g. cell staining buffer

6. Resuspend and proceed with further procedures.

Lysis of Mouse Spleen:

1. Harvest mouse spleen and prepare a single cell suspension.

2. Pellet the cells by centrifugation and aspirate the supernatant.

3. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water and resuspend the pellet in 5 ml of 1X Lysis Buffer. Incubate on ice for 4-5 minutes.

4. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS.

5. Centrifuge 350 x g for 5 minutes and discard the supernatant.

6. Resuspend the pellet in the appropriate buffer, e.g. cell staining buffer.

7. Count cells, adjust density, and proceed with cell staining procedures.

Specifications

Sinónimos

RBC Lysis Buffer | Lysis Buffer

Especificações e pureza

sterile-filtered, BioReagent, for cell culture, 10x

Estabilidade e armazenamento

Store at 2-8°C for 12 months. Do not freeze.

Condições de armazenamento de armazenamento

Store at 2-8°C

Enviado em

Wet ice

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Grau

BioReagent, Filtrado esterilizado, para cultura celular

Nomes e identificadores

• PH: 7.4