SC-55858 , CAS No.179464-49-4

CAS: 179464-49-4 Cat. No.: S1453015 Fórmula: C₁₈H₃₇Cl₂MnN₅ Peso molecular: 449.36

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Visão geral

SC-55858 is an effective superoxide dismutase simulator. SC-55858 increased heart rate and decreased mean arterial pressure and left ventricular systolic and end-diastolic pressures in conscious dogs.

Specifications

Condições de armazenamento de armazenamento

Store at -20°C

Enviado em

Ice chest + Ice pads

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Nomes e identificadores

• Peso molecular: 449.36

Application Protocols

No tested applications, assay formats, or recommended dilutions are provided in the Product Data for this item. The following general protocols apply to small-molecule research compounds and should be adapted by the user.

General small-molecule stock handling:

• Prepare a 10–50 mM stock in DMSO (as solubility allows). Vortex, briefly sonicate, and warm to 37°C if needed to dissolve.
• Aliquot into low-bind tubes to avoid repeated freeze–thaw and adsorption losses. Store at −20°C.

Assay setup (generic):

• Biochemical assays: Start with a 10-point half-log dilution series; keep final DMSO ≤0.1–0.5% v/v. Include vehicle controls.
• Cell-based assays: Titrate across a wide range (e.g., 0.01–30 µM) to establish concentration–response; confirm cell tolerance to the vehicle.
• Stability check: After assay incubation, analyze a representative well by LC–MS to confirm compound integrity where feasible.

These are general recommendations and not validated for SC-55858 specifically. Optimize for your assay format and consult the CoA/Spec Sheet for any item-specific notes if available.

Biological Roles

Item-specific biological target(s), mechanism of action, or pathway engagement are not provided in the Product Data. Accordingly, no claims are made here regarding activity, selectivity, or potency.

General considerations for small-molecule research tools:

• Role: Such compounds are commonly used to modulate enzymes, receptors, ion channels, transporters, or signaling nodes in cell/biochemical assays to study pathway function.
• Controls: Include appropriate vehicle controls (matching DMSO or ethanol), inactive analogs (if available), and orthogonal probes when interpreting phenotypic outcomes.
• Concentration setting: Start with a broad range (e.g., 10-point half-log dilutions) to map response curves, keeping final co-solvent ≤0.1–0.5% where feasible.
• Off-target risk: Without characterized selectivity data, interpret results cautiously and confirm with secondary assays.
• Stability: Verify chemical stability in assay buffer and over incubation time (e.g., by LC–MS) to avoid artifacts due to degradation.

Note: For any specific biological role, consult primary literature or the item’s CoA/Spec Sheet if it includes the structure or citations. This product is provided strictly for research use only.

Buffer Applications

This item is a small-molecule life-science reagent and is not a buffering agent. It is not typically used to control solution pH or ionic strength.

Practical notes when using in buffers (general):

• Ensure compatibility of the compound with your chosen buffer (e.g., PBS, HEPES, Tris). Some compounds may degrade or change ionization state with pH.
• Maintain low organic co-solvent percentages to preserve buffer function and cell viability where applicable.

For recipes and pH ranges of biological buffers, refer to dedicated buffering agents rather than this product.

Green Alternatives

Because SC-55858 is supplied as a life-science research compound (presumably used as a probe/ligand) rather than as a bulk solvent or reagent, conventional “green replacement” discussions (e.g., swapping chlorinated solvents) are less applicable. Nonetheless, you can reduce environmental impact during handling and assays:

• Solvent minimization: Use the highest practical stock concentration in DMSO or ethanol to reduce total organic solvent volume introduced into assays.
• Aqueous delivery: Where compatible, deliver via aqueous buffers employing minimal co-solvent (0.1–0.5% v/v) or complexing agents (e.g., cyclodextrins) to lower organic load.
• Waste segregation: Collect DMSO- and alcohol-containing waste separately to optimize disposal pathways.
• Micro-scale workflows: Adopt miniaturized assay formats (96/384/1536-well) to reduce chemical consumption.

Comparison of delivery media (general, not item-specific):

• DMSO: Excellent solvency; low volatility; facilitates high-concentration stocks; manage cytotoxicity at >0.5–1% final.
• Ethanol: Renewable feedstock; higher volatility may increase emissions; generally tolerated at ≤0.5% in many cell assays.
• Aqueous + cyclodextrin: Enables organic solvent reduction; confirm that the carrier does not perturb assay readouts.

For synthesis-centric green alternatives (if applying SC-55858 as a substrate/reagent), consult solvent selection guides (e.g., CHEM21/ACS GCI) once the compound’s reactivity and solubility profile are known.

Pharmaceutical Uses

No pharmacopeial status, excipient role, or formulation use is specified for this item. It is supplied strictly for research use only and is not intended for human or veterinary use, diagnostic procedures, or clinical applications.

General formulation-related guidance for research use (not clinical):

• Stock solutions: Prepare concentrated DMSO stocks, aliquot, and store at −20°C to support reproducible dosing in preclinical in vitro assays.
• Vehicle preparation: For in vivo exploratory studies conducted under appropriate approvals, vehicles commonly include aqueous buffers with a small percentage of DMSO and/or solubilizers (e.g., PEG-400, Tween 80, Captisol). Confirm compound stability and tolerability; this is user-validated and outside the scope of the Product Data.

Important: Aladdin Scientific supplies this item for laboratory research only. No claims are made regarding safety, efficacy, or suitability for therapeutic use.

Physical Properties

Item-specific physicochemical data are not provided in the Product Data. Do not assume values for critical parameters. Use the latest CoA/Spec Sheet for experimental specs.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.
• LogP/logD: Not specified for this item; refer to CoA/Spec Sheet.
• pKa: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/practice; not item-specific):

• Many bioactive small molecules are sparingly soluble in water but dissolve well in polar aprotic solvents (e.g., DMSO) or alcohols (e.g., ethanol). Where aqueous use is required, co-solvents or complexing agents (e.g., 2-hydroxypropyl-β-cyclodextrin) can help.
• If the compound is a solid at room temperature, warming to 37–40°C and brief sonication often assists dissolution in DMSO/EtOH.
• Always verify actual solubility and stability with the specific lot documentation before preparing concentrated stocks.

Quality and Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

Interpretation and guidance:

• When a purity percentage or grade (e.g., ≥98%, HPLC) is listed on the CoA/Spec Sheet, it typically reflects chromatographic area normalization unless otherwise noted. Verify whether purity excludes solvents/salts.
• If spectroscopic or chromatographic identity tests are reported (1H NMR, 13C NMR, HRMS, HPLC/UPLC), use these to confirm suitability for sensitive biological assays.
• UV-Vis background and residual solvents can affect cell-based readouts. HPLC-grade claims, when present, imply low UV-absorbing impurities but do not guarantee absence of bioactive contaminants.
• Stabilizers/inhibitors: Not specified for this item; refer to CoA/Spec Sheet. If stabilizers are present, consider potential assay interference and include solvent-only controls.

Documentation:

• Retain the lot-specific CoA for publications and reproducibility. If needed, request additional analytical documents (HPLC trace, NMR, water/volatile content) from technical support.

Reaction and Applications

This product is listed under life-science reagents and is typically used as a small-molecule tool compound rather than a synthetic reagent. No manufacturer applications beyond category placement are provided.

Research uses (general; not item-specific):

• Chemical biology studies for pathway interrogation, target validation, or phenotypic screening, when the biological target/mechanism is known from literature (not provided here).
• In vitro cell-based assays: assess concentration–response behavior; include vehicle controls matched for solvent percentage.
• In biochemical assays: characterize binding/enzymatic modulation using IC50/Ki where available from literature (not provided in Product Data).

Good practice:

• Establish compound integrity by LC–MS and, if possible, 1H NMR prior to critical studies.
• Prepare serial dilutions freshly from frozen DMSO stocks to minimize degradation and adsorption losses.
• Track lot numbers across experiments to ensure reproducibility.

If your use case is synthetic chemistry: With no structural information provided, we cannot recommend specific transformations. Request the structure and reactivity profile from the CoA before planning synthetic applications.

Reaction Conditions

No manufacturer application data or structure are provided; therefore, no recommended reaction conditions can be specified for this product.

General guidance if chemical modification is attempted (user-validated only):

• Solvents: Choose based on solubility—DMSO, DMF, acetonitrile, or dichloromethane are common for polar/neutral organics; confirm compatibility with the compound’s stability profile.
• Temperature: Start at ambient; escalate cautiously while monitoring by TLC/LC–MS. Avoid prolonged heating if stability is unknown.
• Atmosphere: Use inert gas (N2/Ar) when performing moisture- or air-sensitive transformations.
• Workup: Quench carefully and use extraction media aligned with the compound’s polarity; minimize emulsions by salt addition where needed.

These are general practices and not item-specific recommendations. Consult the CoA and, if available, literature for SC-55858 before planning any chemistry.

Safety and Handling

Authoritative safety information resides in the Safety Data Sheet (SDS). The following summarizes general good laboratory practice for small-molecule research reagents when item-specific data are unavailable.

Item-specific hazard data:

• GHS classification: Not specified for this item; refer to SDS.
• Signal word: Not specified for this item; refer to SDS.
• H-statements: Not specified for this item; refer to SDS.
• Pictograms: Not specified for this item; refer to SDS.

General handling and PPE:

• Handle in a chemical fume hood. Avoid inhalation of dust/vapors and contact with skin, eyes, and clothing.
• Wear appropriate PPE: lab coat, safety glasses or goggles, and chemical-resistant gloves (e.g., nitrile). Change gloves regularly when working with DMSO solutions due to enhanced dermal absorption potential of many solutes.

Storage and incompatibilities:

• Storage condition (from Product Data): Store at −20°C. Keep container tightly closed, desiccated, and protected from light if the compound is light-sensitive (verify on CoA/SDS).
• Avoid strong oxidizers and strong acids/bases unless compatibility is known. Use inert atmosphere for air/moisture-sensitive materials.

First-aid overview (general):

• Skin/eye contact: Rinse with water for at least 15 minutes; remove contaminated clothing; seek medical attention as needed.
• Inhalation: Move to fresh air; seek medical advice if symptoms persist.
• Ingestion: Rinse mouth; do not induce vomiting; seek medical attention.

Waste disposal:

• Collect residues and contaminated consumables as hazardous chemical waste per institutional and local regulations.

Solvent Selection

Item-specific solubility is not provided. The following best practices apply to hydrophobic bioactive small molecules used in life-science research.

Recommended approach:

• Primary solvent: DMSO is the default for preparing concentrated stock solutions (e.g., 10–50 mM), given its broad solubilizing power and compatibility with most in vitro assays at low final percentages (<0.1–0.5%).
• Secondary options: Ethanol or methanol may be used where DMSO is undesirable; verify assay tolerance. For aqueous use, deploy co-solvent systems (e.g., DMSO or EtOH at 0.1–1%) and/or carriers like 2-hydroxypropyl-β-cyclodextrin.
• pH adjustment: If the compound bears ionizable groups (unknown here), modulating pH can aid solubility; confirm stability across pH before use.

Practical tips:

• Warm and briefly sonicate to accelerate dissolution; avoid prolonged high-temperature exposure unless stability is confirmed.
• Filter sterilize (0.22 µm PTFE) only after complete dissolution; many bioactives adsorb to nylon—prefer PTFE or PVDF.
• Adsorption minimization: Use low-bind tubes and deliver in minimal volumes to plasticware.

Comparison (general):

• DMSO: Highest solubilizing capacity; may carry compounds across membranes; keep final assay % low.
• Ethanol: Volatile and less cytotoxic at low %; solubility may be lower.
• Aqueous buffers: Lowest solubility for hydrophobes; consider cyclodextrins or micelles where appropriate.

Always confirm actual solubility and stability with the lot-specific CoA/Spec Sheet.

Storage and Reconstitution

• Storage conditions (from Product Data): Store at −20°C.
• Shipping (from Product Data): Shipped in ice chest with ice pads to maintain a cold chain.

Reconstitution (general guidance for small-molecule solids/powders; adjust per CoA):

• Solvent choice: Begin with anhydrous DMSO to prepare a concentrated stock (e.g., 10–50 mM), then dilute into assay buffer immediately before use. If DMSO is unsuitable, consider ethanol or aqueous buffer with a suitable solubilizer (e.g., cyclodextrin), pending stability.
• Technique: Allow vial to equilibrate to room temperature in a desiccator before opening to avoid moisture condensation. Weigh quickly, cap promptly. Dissolve with vortexing, brief sonication, and mild warming (≤37–40°C) if required.

Aliquoting and stability:

• Prepare single-use aliquots to minimize freeze–thaw cycles. Store tightly sealed, protected from ambient moisture and light as appropriate (check CoA for light sensitivity).
• Working solutions in aqueous buffers are generally less stable than DMSO stocks; prepare fresh and use promptly.

Expiration and documentation:

• Follow any lot-specific retest/expiry date on the label or CoA. Record preparation date, solvent, and concentration on each aliquot.

Note: Appearance, exact solubility, and stability data are not specified for this item; refer to the lot-specific CoA/Spec Sheet for definitive guidance.

Research Use: For research use only.

Structure and Identity

• Product name: SC-55858 (SKU: S1453015)
• CAS: 179464-49-4
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

Structural features and description (general):

• Specific ring systems, functional groups, and stereochemistry are not provided in the Product Data. Please consult the CoA/Spec Sheet for definitive structural identifiers.
• Category context: Listed under life-science reagents; this SKU likely corresponds to a small-molecule research tool compound rather than a macromolecule or polymer.

Notes:

• For unambiguous identification in manuscripts or regulatory submissions, include CAS (179464-49-4) together with supplier SKU (S1453015) and, when available, SMILES/InChI from the CoA.

Synthetic Utility

This product is positioned as a life-science research compound rather than a building block or reagent. Without structural information, specific synthetic transformations cannot be recommended.

General notes:

• If the structure is available from the CoA or literature, assess functional groups for reactivity (e.g., halides for cross-coupling, carbonyls for condensations, amines for acylation/alkylation). Absent that, treat the material as a final small-molecule tool compound not intended for further derivatization.
• For analytical confirmation during any derivatization attempts, employ LC–MS and NMR to monitor conversions and detect potential degradation.

If you intend to use SC-55858 as a synthetic intermediate, please obtain the full structural identifiers (SMILES/InChI) and any protecting-group/stability information from the CoA/Spec Sheet before planning routes.

Target Specificity

• Target/epitope: Not applicable; this product is not an antibody or protein reagent.
• Intended use: Small-molecule life-science research compound. No item-specific biological target, selectivity, or potency data are provided in the Product Data.

Guidance:

• If you require target specificity details (e.g., enzyme/receptor class, Ki/IC50, selectivity panel), consult the lot-specific CoA/Spec Sheet or primary literature referencing CAS 179464-49-4.
• Validate activity in your system with appropriate positive/negative controls and orthogonal assays.