Seed Viability Assay Kit (TTC Method)

Cat. No.: S1522018

GRAU & PUREZA BioReagent

Visão geral

Seed viability (often referred to simply as seed vigor) refers to the potential ability of a seed to germinate or the vitality of the embryo. The level of seed viability determines seed quality and practical value, and it influences the amount of seed required for sowing. A common method for determining seed viability is the germination test, where seeds are allowed to imbibe water and germinate under suitable conditions, and the percentage of germinated seeds out of the total tested seeds is counted within a specified number of days. TTC is a redox indicator with a standard oxidation-reduction potential of 80 mV. It forms a colorless solution when dissolved in water, and upon reduction, it produces red, water-insoluble triphenylformazan. This compound is relatively stable and not easily oxidized. Therefore, TTC is widely used as a hydrogen acceptor in enzymatic studies. The amount of TTC reduction reflects dehydrogenase activity, thereby serving as an indicator for assessing the viability of seeds from plants such as wheat, mung beans, rice, rapeseed, and peanuts. When the TTC staining solution penetrates the cells of the embryo, the embryo is stained red. When embryo viability decreases, respiration weakens significantly, dehydrogenase activity declines, and the color change in the embryo is less pronounced. When the embryo is non-viable, it remains unstained. Thus, the degree of staining indicates the level of seed viability. This product is intended for research use only and is not for use in diagnostic procedures or other purposes.

Especificações

Especificações e pureza

BioReagent

Grau

BioReagent

Aplicação

Cell Metabolism

Armazenamento e envio

Condições de armazenamento de armazenamento

Store at 2-8°C,Protected from light

Enviado em

Wet ice

Estabilidade e armazenamento

Store at 2-8℃ for 6 months. Store in the dark.

Perguntas frequentes

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at 2–8 °C, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.