Soybean-Casein Digest Medium (USP) - BioReagent

Cat. No.: S1528404

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Visão geral

For sterility testing. Preparation method: Weigh 30.0 g of this product in 1 L of distilled or deionized water (the volume may be scaled up or down proportionally), dissolve with gentle warming, dispense, autoclave at 121°C for 15 minutes, and set aside for use.

Specifications

Especificações e pureza

BioReagent

Condições de armazenamento de armazenamento

Room temperature

Enviado em

Normal

Grau

BioReagent

Product Line

biological_products

Application Protocols

The following are general, literature-based guidelines. They are not item-specific specifications. Always follow your institutional SOPs and consult the CoA for lot performance.

A. Preparation of Soybean-Casein Digest Broth (literature/general)

• Weigh the manufacturer-recommended amount of dehydrated medium per liter of purified water (check product label/CoA).
• Dissolve with stirring; adjust pH only if required by method.
• Dispense into containers/closures suitable for your test.
• Sterilize by autoclaving (e.g., 121 °C, 15 min). Cool to use temperature.

B. Preparation of Soybean-Casein Digest Agar (literature/general)

• As above, using the agar-containing formulation or adding agar per validated recipe.
• Heat to dissolve agar fully; autoclave; cool to ~45–50 °C; pour plates aseptically.

C. Growth Promotion Test (compendial concept; literature/general)

• Inoculate with ≤100 CFU of compendial strains (e.g., Staphylococcus aureus, Bacillus subtilis, Candida albicans) as applicable.
• Incubate at method-specified temperatures (e.g., bacteria 30–35 °C; fungi 20–25 °C) for prescribed times.
• Acceptance: Demonstrate characteristic growth comparable to a previously qualified batch (follow pharmacopeial criteria/SOP).

D. Sterility/Bioburden Testing (literature/general)

• Use as sample diluent or recovery medium; include negative controls.
• Document sample volumes, incubation times, and any neutralizers used.

Item-specific preparation strength and pH: Not specified for this item; refer to CoA/Spec Sheet.

Biological Roles

As a complex nutrient medium, Soybean-Casein Digest Medium serves to support microbial physiology rather than participate in a specific biochemical pathway.

• Function (literature/general): Provides a balanced pool of peptides, amino acids, carbohydrates, and minerals that collectively support ATP generation, biosynthesis (proteins, nucleotides, cell wall), and growth of diverse bacteria and many fungi.
• Nutrient profile (general):
  • Casein digests supply readily assimilable peptides and amino acids, including branched-chain and aromatic residues.
  • Soybean digests contribute peptides, carbohydrates, and growth factors.
  • Mineral salts help maintain osmotic balance and enzyme function.
• Microbial scope (literature/general): Broadly non-selective; supports common aerobes and facultative anaerobes such as Staphylococcus spp., Bacillus spp., Enterobacteriaceae, Pseudomonas spp., as well as many molds/yeasts when conditions permit. Extremely fastidious organisms may require enriched media.
• Physiological considerations:
  • Near-neutral pH and isotonicity favor robust growth and recovery after stress.
  • Medium composition supports recovery of low-inoculum contaminants during sterility/bioburden testing due to non-inhibitory nature.
• Additives (per SOP; literature/general): Selective agents (antibiotics), neutralizers (e.g., polysorbates, lecithin), or indicators may be added for specific monitoring tasks. Any additive must be validated not to compromise recovery.

Note: Exact composition and any animal-origin-free status are lot/manufacturer dependent; consult the CoA/Spec Sheet for item-specific details.

Buffer Applications

This product is not a dedicated buffering agent or defined buffer system. However, the prepared medium typically exhibits near-neutral pH and modest buffering capacity due to its peptone and salt content (literature/general).

• Typical final pH (prepared medium): ~7.3 at 25 °C (literature). Item-specific value: Not specified for this item; refer to CoA/Spec Sheet.
• Use in buffering workflows: Not commonly used for precise pH buffering. For experiments requiring exact pH control or ionic strength, select a defined buffer system (e.g., phosphate, HEPES, MOPS) and overlay with nutrient sources as needed.
• Practical notes (literature/general):
  • Adjusting pH of complex media can alter growth characteristics; any adjustment should be validated.
  • For electrophoresis or enzyme assays, defined buffers are preferable; this medium’s composition and UV absorbance are undefined and variable.

For strict buffer applications, see dedicated buffering reagents. Utilize this medium for its intended role as a general-purpose, non-selective growth medium.

Green Alternatives

Sustainability considerations for Soybean-Casein Digest Medium focus on source materials, energy, and waste in microbiological workflows rather than substitution by an “organic solvent” alternative.

• Source/ethics (literature/general): Contains both soybean (plant-derived) and casein (animal-derived) digests. For animal-free policies, consider vegetable-only peptone media that emulate TSB performance without casein components. Trade-off: growth of some fastidious organisms may be reduced; confirm with growth-promotion tests.
• Energy use: Autoclaving (e.g., 121 °C for 15 min) is energy intensive. Optimize by batching loads, validating shortest acceptable cycles, and using insulated vessels.
• Waste reduction: Prepare only the volume needed; store dehydrated medium in airtight containers to maximize shelf life and reduce discard due to moisture uptake.
• Packaging: Choose larger pack sizes when practical to reduce packaging waste; ensure desiccant recovery and recycling where available.

Illustrative comparison (literature/general):

• Standard Soybean-Casein Medium vs. Vegetable-only Peptone Medium
  • Animal content: present vs. none.
  • Growth spectrum: very broad vs. broad (organism-dependent).
  • Ethical/sourcing: mixed animal/plant vs. plant-only.
  • Validation burden: baseline vs. requires additional growth-promotion equivalency.

Bottom line: When shifting to greener/animal-free alternatives, perform side-by-side growth-promotion studies with your target organisms and matrices to verify suitability.

Pharmaceutical Uses

No therapeutic/clinical claims are made. In pharmaceutical and biomanufacturing laboratories, Soybean-Casein Digest Medium is used as a process and quality control reagent (literature/general):

• Sterility testing and growth promotion in compendial microbiological methods (USP/EP/JP), including testing of sterile products, components, and environments. Confirm lot-specific performance with CoA and perform growth-promotion per your SOP.
• Environmental monitoring: When solidified with agar, used for surface contact plates, settle plates, and active air sampling media. Neutralizers (e.g., polysorbate/lecithin) may be incorporated if validated.
• Bioburden testing and in-process monitoring: Non-selective recovery of low-level contaminants in water systems, raw materials, filters, and final rinse solutions.
• Media fills/process simulations: As a nutrient-rich broth to detect process contamination in aseptic operations (use per validated protocols).

Regulatory note (general): “USP” in the name denotes alignment with a pharmacopeial medium type; it does not by itself attest that a given lot meets all compendial acceptance criteria. For regulated uses, document:

• Water quality used for reconstitution (Purified Water/WFI as applicable),
• Sterilization cycle parameters,
• Growth-promotion and negative controls,
• Lot traceability and storage controls.

Item-specific pharmacopeial compliance status: Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Because this is a complex dehydrated medium, many conventional single-compound properties do not apply. Where helpful, literature/general values are provided for reference and must not be taken as item-specific specifications.

• Physical state/appearance: Typically a free-flowing powder (dehydrated medium) prior to reconstitution (general). Item-specific value: Not specified for this item; refer to CoA/Spec Sheet.
• Color/odor: Light to tan powder with mild peptone odor (general). Item-specific value: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Readily soluble in purified water to form a clear to slightly hazy medium (literature/general). Item-specific value: Not specified for this item; refer to CoA/Spec Sheet.
• pH (of prepared medium): Typically ~7.3 at 25 °C (literature, USP-style formulations). Item-specific value: Not specified for this item; refer to CoA/Spec Sheet.
• Density, refractive index, logP, pKa, boiling/melting point: Not applicable/reported for this complex mixture; Not specified for this item; refer to CoA/Spec Sheet.
• Osmolality (prepared broth): Dependent on formulation and lot; (literature) TSB-type media are near isotonic. Item-specific value: Not specified for this item; refer to CoA/Spec Sheet.

Preparation notes (literature/general): Medium dissolves in cold or warm water with gentle stirring; sterilize by autoclaving (e.g., 121 °C for 15 minutes). If agar is present, heating to dissolve and clear is required prior to sterilization. Always verify clarity and pH after sterilization.

Quality and Grades

• Item grade: BioReagent (per Product Data). BioReagent-grade materials are suitable for general laboratory and biological workflows, emphasizing consistency and low levels of inhibitory contaminants. This is not a clinical/diagnostic grade.
• “USP” in the product name reflects the Soybean-Casein Digest Medium described in pharmacopeial literature (e.g., USP) for microbiological quality control (literature). However, item-specific compendial conformity tests, growth-promotion results, and tolerances are Not specified for this item; refer to CoA/Spec Sheet.
• What BioReagent grade means in practice:
  • Raw materials and processing suitable for routine microbiological culture and QC in research/manufacturing environments.
  • Emphasis on reproducibility across lots; however, verify lot-specific performance (e.g., growth promotion) before critical use.
• Typical QC attributes for such media (literature/general; not guaranteed for this item unless stated on CoA):
  • Appearance, pH of prepared medium, clarity/haze.
  • Growth promotion testing with a panel of compendial challenge strains at low inoculum.
  • Sterility of dehydrated medium (bioburden control) and absence of inhibitory substances.
• Stabilizers/additives: Not specified for this item; refer to CoA/Spec Sheet.
• UV/trace contaminants: Not specified for this item; refer to CoA/Spec Sheet.

Recommendation: For compendial/regulated workflows, confirm the Certificate of Analysis for growth-promotion and any USP-referenced acceptance criteria relevant to your application.

Reaction and Applications

This product is not a chemical reagent for synthetic reactions. Its applications are microbiological and QC-focused.

Key research and quality-control applications (literature/general; expand on typical USP uses):

• Growth and maintenance of a wide variety of aerobic and facultative anaerobic bacteria, and many fungi, due to a balanced supply of peptides, amino acids, carbohydrates, and minerals.
• Sterility testing medium in compendial methods (USP/EP/JP contexts), commonly as Soybean-Casein Digest Medium for detecting microbial contamination in products. Growth-promotion tests challenge the medium with low-level inocula of standard strains.
• Environmental monitoring and bioburden testing when prepared as broth (TSB) or solidified with agar (TSA) for surface/air sampling and incubation.
• Process simulation/media fills (when supplemented/used per SOP) to evaluate aseptic operations (general statement; follow site procedures).
• General-purpose recovery medium following stress/exposure (e.g., disinfectants, filtration), often chosen for its non-selective profile.

Practical tips (literature/general):

• Prepare under aseptic conditions; verify pH and clarity post-autoclave.
• For anaerobes or microaerophiles, modify atmosphere or supplement (e.g., reducing agents) per method.
• For fungi/yeast enrichment, incubation at appropriate temperatures and times is critical; antibiotics may be added for selectivity if validated.

Note: Always follow your validated SOPs and consult the CoA for lot-specific performance.

Reaction Conditions

Conventional organic reaction conditions are not applicable. The relevant “conditions” concern media preparation and incubation for microbiological work (literature/general guidance):

• Reconstitution: Dissolve the dehydrated medium in high-purity water with gentle stirring until fully dissolved. Heat may be applied if agar is present to achieve clarity.
• Sterilization: Autoclave (e.g., 121 °C, 15 min at 15 psi). Do not over-autoclave to avoid caramelization or Maillard reactions that may darken medium and reduce performance.
• pH: Typical prepared pH ~7.3 at 25 °C (literature). Adjust only if specified by the method and validate impact on growth.
• Incubation conditions: Temperature and time depend on target organisms and test method (e.g., 20–25 °C for fungi/yeasts; 30–35 °C or 32.5 ± 2.5 °C for many bacteria in compendial tests; durations 2–7+ days per SOP; literature/general).
• Atmosphere: Aerobic by default; use anaerobic jars or CO2 incubators as needed for specific organisms.
• Neutralizers/selectivity: Add polysorbate, lecithin, or antibiotics if required by environmental monitoring methods; validate recovery.

Item-specific preparation concentration, pH tolerances, and growth-promotion results: Not specified for this item; refer to CoA/Spec Sheet.

Safety and Handling

No GHS hazard classification is provided in the product data. For authoritative information, consult the Safety Data Sheet (SDS) for this SKU.

• GHS classification, signal word, H-statements, pictograms: Not specified for this item; refer to SDS.
• Likely hazards (general guidance for dehydrated culture media):
  • Nuisance dust may cause mechanical irritation to eyes, skin, and respiratory tract.
  • May contain animal-derived casein digest; handle to avoid allergen exposure (general).
• PPE (typical laboratory practice): Lab coat, gloves, safety glasses, and dust control (mask/respirator) if airborne dust may be generated. Use in a clean area to prevent contamination of media and environment.
• Handling tips:
  • Avoid inhalation of dust; minimize dust formation during weighing and dispensing.
  • Use aseptic technique to prevent microbial contamination when reconstituting.
  • Do not ingest. For research use only.
• Storage incompatibilities (general): Keep dry and away from strong oxidizers. Avoid humid environments to prevent caking or premature degradation.
• First-aid (overview; defer to SDS):
  • Eye/skin contact: Rinse with water. Remove contaminated clothing.
  • Inhalation: Move to fresh air. Seek medical advice if irritation persists.
  • Ingestion: Rinse mouth, do not induce vomiting; seek medical advice.

Always defer to the SDS and institutional biosafety procedures for complete safety guidance.

Solvent Selection

This product is a dehydrated culture medium intended to be reconstituted in water. Solvent selection considerations focus on water quality and compatibility rather than organic solvent choice.

• Primary solvent: Purified/DI water (Type I/II per ASTM/CLSI) or water meeting pharmacopeial “Purified Water/WFI” requirements when used in regulated settings (literature/general).
• Miscibility: Media are fully water-soluble at the intended preparation strength; organic solvents are not appropriate for routine reconstitution.
• Water quality notes (literature/general):
  • Avoid residual sanitizers (e.g., chlorine, ozone), heavy metals, or high TOC that may inhibit microbial growth.
  • Degassing is optional; not typically necessary. Use freshly produced water to minimize microbial background.
• pH control: Adjust only if allowed by your SOP; typical final pH is near neutral (literature). Over-adjustment can impair growth.
• When to choose this medium vs alternatives (literature/general):
  • Select Soybean-Casein Digest Medium for broad-spectrum, non-fastidious bacterial and fungal growth and for compendial sterility/environmental monitoring workflows.
  • Consider selective/differential media if targeting specific taxa, or vegetable-only peptones if avoiding animal-derived components.

Comparison (literature/general):

• vs. Nutrient Broth: richer peptide profile and carbohydrate content; broader support for aerobes/anaerobes.
• vs. Brain Heart Infusion (BHI): TSB/Soybean-Casein is less animal-tissue rich but still robust; BHI may better support very fastidious organisms.

Storage and Reconstitution

• Storage conditions (as supplied): Room temperature (per Product Data). Keep container tightly closed, dry, and protected from humidity and direct sunlight. Use desiccant when available. Avoid temperature extremes.
• Shipping conditions: Normal (per Product Data).
• Shelf life/expiry: Not specified for this item; refer to CoA/Spec Sheet.

Reconstitution and sterilization (literature/general guidance; verify label/CoA for exact amounts):

• Broth: Dissolve the manufacturer-recommended grams per liter of purified water with stirring. If needed, adjust pH minimally. Distribute to containers; autoclave (e.g., 121 °C, 15 min). Cool before use. Inspect for clarity and correct pH.
• Agar: Heat to fully dissolve before autoclaving. After sterilization, cool to ~45–50 °C and pour plates aseptically.
• Aseptic handling: Work in a clean environment (e.g., biosafety cabinet) to prevent contamination.

Use and storage after preparation (literature/general):

• Store poured plates at 2–8 °C in sealed sleeves to prevent drying; equilibrate to room temperature before use. Prepared broth can be stored refrigerated; avoid prolonged storage that may alter performance. Do not use if contamination, precipitation, or significant color change is observed.

Note: This product is for research use only. Item-specific preparation strength, pH targets, and growth-promotion results are Not specified for this item; refer to CoA/Spec Sheet.

Structure and Identity

Soybean-Casein Digest Medium (USP) is a complex, undefined microbiological growth medium (often synonymous with Tryptic Soy Broth/Agar in compendial contexts). As a mixture of peptones/digests, it does not have a single discrete chemical structure.

• Product type: Complex culture medium composed of enzymatic digests of soybean meal and casein, plus mineral salts and, for the agar form, a gelling agent (agar). Exact composition proportions vary by manufacturer/lot.
• Molecular formula: Not applicable (complex mixture). Item-specific value: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not applicable (mixture). Item-specific value: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES / InChI / InChIKey: Not applicable for complex mixtures of peptides, amino acids, carbohydrates, and salts.
• Structural features (descriptive):
  • Contains short peptides, free amino acids, carbohydrates, and minerals derived from soybean and casein digests (general literature description).
  • No defined stereochemistry or single ring system; constituents include a distribution of peptide sequences and small molecules typical of enzymatic hydrolysates (general literature).
• Synonyms (literature): Tryptic Soy Broth (TSB) or Tryptic Soy Agar (TSA) when agar is included; also “Soybean-Casein Digest Medium” per USP monographs (literature).

Note: This listing is for research/laboratory use only. Consult the lot-specific Certificate of Analysis to confirm compendial alignment and any identity tests performed.

Synthetic Utility

Not applicable as a chemical building block. Soybean-Casein Digest Medium is a complex nutrient mixture intended for microbiological culture and quality control.

• It does not participate in defined organic reactions, nor does it serve as a reagent/catalyst for synthetic transformations.
• If nutrients or peptides from the medium are needed in chemical workflows (e.g., as nitrogen sources in biotransformations), use is strictly biological/fermentation-oriented and should be validated case-by-case.

For chemical synthesis applications, select defined reagents and solvents from appropriate categories. Refer instead to the sections on Reaction & Applications and Application Protocols for microbiology-focused usage.

Target Specificity

Not applicable. This product is a non-selective, general-purpose culture medium and is not an antibody, probe, or targeted reagent.

• Antigen/epitope, clone, isotype, species reactivity: Not applicable.
• Intended use profile: Broad recovery of diverse aerobic and facultative anaerobic bacteria (and many fungi) rather than specificity toward a particular target organism (literature/general).

Perguntas frequentes

How should this product be stored?

Store at room temperature.