Universal Tissue Single-Cell Dissociation Enzyme

Cat. No.: U1507399

GRAU & PUREZA EnzymoPure™

Visão geral

This product is a universal tissue single-cell dissociation enzyme, which can be used for the preparation of single-cell suspensions from various organ tissues such as heart, liver, spleen, lung, and kidney of animals including humans, mice, and rats. Through the gentle enzymatic hydrolysis of a multi-component composite enzyme, various organ tissues are efficiently dissociated into single-cell suspensions, achieving high yield while maintaining high cell viability. The resulting single cells are suitable for downstream experiments including single-cell sequencing, flow cytometry analysis, flow cytometric sorting, primary cell culture, and organoid 3D culture. 

Component List

Product Advantages

1. Ready-to-Use (One-Step Operation)

Simply reconstitute the provided enzyme powder with the supplied buffer for immediate use in downstream experiments. Unlike imported competitors that require separate reconstitution of 3-4 enzymes in different solutions, volume calculation, and mixing, this product eliminates the need for weighing, complex calculations, and multi-step operations—significantly reducing the risk of errors.

2. Multi-Component Composite Enzyme

Independently developed and manufactured, this product contains over 8 types of composite enzymes tailored to the extracellular matrix components of different tissues. It achieves more efficient digestion of intercellular matrices compared to single-use collagenase IV, DNase, or 3-4 component mixed enzymes from imported brands. Key benefits include shorter digestion time, higher cell yield, superior cell viability, intact antigen epitopes, and more competitive pricing due to independent R&D and production.

3. Short Digestion Time

Leveraging over 8 types of self-developed tissue-specific dissociation enzymes, most organ tissues are fully dissociated within 10-15 minutes. In contrast, single collagenase IV, DNase, or imported mixed enzymes typically require ~1 hour, and sometimes 2-3 hours, for complete dissociation.

4. High Cell Yield

The multi-component composite enzyme ensures thorough digestion of intercellular matrices, maximizing the release of single cells with minimal tissue clump residues—resulting in higher overall cell yield.

5. Superior Cell Viability

Tissue-specific composite enzymes enable gentle dissociation, causing less cell damage than trypsin. Combined with shorter digestion time, the product minimizes in vitro cell exposure and avoids over-digestion, preserving high cell viability and intact antigen epitopes.

6. Intact Antigen Epitopes

Efficient digestion reduces both processing time and in vitro cell exposure, minimizing tissue/cell damage while maintaining structural integrity and high viability. Intact antigen epitopes facilitate antibody binding and staining, making the dissociated single cells ideal for flow cytometry analysis and sorting.

Usage Instructions

1. Reconstitute Enzyme Powder

Perform this step when first using the kit:

Centrifuge the vial of Enzyme Powder A briefly to collect all powder at the bottom.

Use a pipette to transfer Reconstitution Buffer B into the vial of Enzyme Powder A, repeatedly pipetting to resuspend the powder. For easier transfer, trim the tip of the pipette if necessary to ensure complete transfer of the powder into Buffer B.

Cap the vial tightly and invert to mix until the powder is fully dissolved (use a shaker if needed). The fully reconstituted enzyme solution will appear clear and slightly brown.

2. Filter and Aliquot Dissociation Enzyme

After complete reconstitution:

For sterile tissue dissociation (e.g., cell culture), filter the enzyme solution through a 0.22μm sterile filter using a syringe, then aliquot.

Recommended aliquot volume: 5mL per vial (reuse vial A if desired).

Store aliquots at -20℃ or -80℃ immediately; stable for 6 months. Unused aliquots can be refrozen for future use, but avoid more than 3 freeze-thaw cycles (each cycle reduces enzyme activity—adjust volume accordingly to compensate for reduced activity).

3. Enzymatic Digestion Procedure

(1) Pre-Experiment Preparation

Prepare crushed ice; thaw the single-cell dissociation enzyme and digestion stop solution on ice.

Preheat the single-cell dissociation instrument (if used); pre-cool PBS, sterile surgical tools (ophthalmic scissors, forceps), 40μm sterile cell strainers, centrifuge tubes, and centrifuge.

(2) Tissue Collection

Collect tissues under sterile conditions and immediately place in cold PBS. Process within 4 hours if possible; for longer storage, use High-Activity Tissue Preservation Solution.

(3) Tissue Washing

Wash tissues 1-2 times with cold PBS to remove blood, necrotic tissue, and other impurities. Weigh and record the tissue mass.

(4) Tissue Mincing

Transfer washed tissues to a dissociation tube and mince into 1-2mm³ pieces (mush-like consistency) using sharp ophthalmic scissors. Finer mincing ensures more efficient digestion and shorter processing time. If using a single-cell dissociation instrument, mincing can be less rigorous while maintaining good dissociation results.

(5) Tissue Digestion

Add an appropriate volume of the reconstituted dissociation enzyme to the tube and invert to mix thoroughly with the minced tissue.

① If a tissue single-cell dissociation instrument is available, perform single-cell dissociation according to the instrument's operating specifications. This product is compatible with all commercial tissue single-cell dissociation instruments (both imported and domestic brands). However, note that its dissociation efficiency is higher than that of user-supplied enzymes or enzymes from other competitors, enabling at least a 50% reduction in dissociation time. Check the dissociation progress every 5-10 minutes—complete dissociation time varies by tissue (ranging from 5 to 50 minutes). Minimize enzymatic digestion time as much as possible while ensuring dissociation efficiency, and terminate digestion immediately once complete.

② If no tissue single-cell dissociation instrument is available, manual dissociation is also feasible:

Ensure tissues are minced strictly as required in the tissue mincing step.

Select a 1.5mL or 5mL EP tube based on the tissue quantity and enzyme volume added. Thoroughly mix the dissociation enzyme with the minced tissue.

Slightly trim the tip of a 1mL pipette with scissors, then vigorously pipette the tissue-enzyme mixture. If pipetting is blocked, the tissue is insufficiently minced—re-mince the tissue or trim the pipette tip further to ensure smooth repeated pipetting.

After 10+ rounds of vigorous pipetting, immediately incubate the tube at 37℃ in an incubator, water bath, or dry bath (a shaking incubator is preferred).

Vigorously pipette the mixture every 5 minutes until all tissue clumps are fully dissociated into a single-cell suspension.

(6) Single-Cell Filtration

Once dissociation is complete, centrifuge the tube briefly (3 seconds) and place on ice.

Pipette the supernatant through a 40μm cell strainer into a new tube (perform all steps on ice).

(7) Stop Digestion

Add an equal volume of Digestion Stop Solution or complete medium to terminate digestion.

(8) Cell Collection

Centrifuge for 5 minutes and discard the supernatant.

(9) Red Blood Cell (RBC) Lysis

Take an appropriate volume of red blood cell lysis buffer to resuspend the cell pellet.

Incubate on ice for 10 minutes, gently pipetting intermittently.

Centrifuge at 300g for 5 minutes at 4℃ and discard the supernatant.

(10) Cell Washing

Centrifuge at 4℃ and discard the supernatant.

(11) Debris Removal

If there is a high amount of debris, a high-efficiency debris remover can be used to eliminate it.

(12) Cell Viability Assessment

Stain with trypan blue or AOPI; viable cell rate should exceed 85%.

(13) Cell Cryopreservation

If downstream experiments are delayed, resuspend single cells in High-Efficiency High-Viability Cell Cryopreservation Solution and store directly at -80℃ (stable for years).

Notes

For research use only.

For sterile culture applications, perform all operations in a biosafety cabinet to avoid contamination.

Digestion must be conducted at 37℃; all other steps should be performed on ice to strictly control temperature.

Adjust digestion time based on tissue quantity. Monitor dissociation progress closely and stop digestion as soon as complete to prevent cell damage.

For high-demand applications (e.g., single-cell sequencing), accelerate the entire process and proceed immediately to library preparation after dissociation.

Store reconstituted enzyme at -20℃ or -80℃ (stable for 6 months). Unused aliquots can be refrozen, but avoid more than 3 freeze-thaw cycles (each cycle reduces activity—increase enzyme volume if needed to maintain digestion efficiency).

Especificações

Product Name

Universal Tissue Single-Cell Dissociation Enzyme

Grau

EnzymoPure™

Especificações e pureza

EnzymoPure™

Tipo de molécula

Enzyme

Product Line

Produtos biológicos

Armazenamento e envio

Condições de armazenamento de armazenamento

Store at 2-8°C

Enviado em

Wet ice

Estabilidade e armazenamento

Store at 2-8℃ long term (12 months). Store at -20℃ after reconstitution.

Application Protocols

No vendor-validated protocols are provided for this specific item. The following are general, literature-based workflow elements to aid planning; adjust to your tissue type and verify against the CoA/Spec Sheet.

• General workflow (literature):

  1. Mince tissue into 1–2 mm pieces in cold buffer.
  2. Add pre-warmed enzyme solution at the desired concentration; incubate with gentle agitation at 4–37 °C depending on sensitivity.
  3. Triturate periodically with wide-bore pipette; monitor turbidity and fragment size.
  4. Add DNase if viscosity increases due to lysis (if compatible).
  5. Quench by dilution and/or serum addition; filter through 70–40 µm strainers.
  6. Wash and resuspend in assay buffer; determine viability and proceed to downstream analyses.

• Tips for sensitive epitopes (literature):

  • Shorten incubation, use lower temperature, or substitute mechanical steps to preserve surface markers for flow cytometry.
  • Keep total processing time minimal to limit stress-response transcriptional changes.

• Documentation:

  • Record lot number, buffer composition, tissue weight, enzyme volume, time, and temperature to ensure reproducibility.

Item-specific concentrations, incubation times, and quenching instructions are Not specified for this item; refer to CoA/Spec Sheet.

Biological Roles

This product belongs to the class of protein enzymes used to cleave extracellular components to generate single cells. Item-specific enzyme identities are not provided.

• General biological function of tissue dissociation enzymes (literature):

  • Proteases and matrix-degrading enzymes hydrolyze peptide bonds in extracellular matrix proteins (e.g., collagens, laminins, fibronectin) and junctional proteins (cadherins, integrins-associated substrates), facilitating release of individual cells.
  • Nucleases (when included) digest extracellular DNA from lysed cells, lowering viscosity and preventing cell clumping.

• Impact on cell biology:

  • Gentle, time-limited digestion preserves cell viability and surface epitopes critical for immunophenotyping and receptor signaling studies.
  • Over-digestion can alter surface proteins or signaling states; careful optimization of exposure minimizes artifacts.

• Downstream compatibility (general considerations):

  • For transcriptomic assays, cold-active or short-duration digests can reduce stress-response gene induction.
  • For functional assays (e.g., electrophysiology), minimized proteolysis of membrane proteins is important; select conditions accordingly.

Note: Specific enzyme composition, co-factors, and inhibitor sensitivities for this item are Not specified; consult the CoA/Spec Sheet for details.

Buffer Applications

As an enzyme reagent, this product is used within standard biological buffer systems rather than serving as a buffer itself.

• Common buffer systems for tissue dissociation (literature guidance):

  • Balanced salts: HBSS or DPBS (with or without Ca2+/Mg2+ depending on enzyme requirements) at pH ~7.2–7.6.
  • Serum/protein additives: 0.1–1% BSA or 2–10% serum for quenching and cell protection; note that serum can inhibit some enzymes.
  • Chelators: EDTA/EGTA may be included for selective disruption of cell–cell adhesions but can inhibit Ca2+-dependent enzymes; use judiciously.
  • Osmolarity: Maintain physiological osmolarity (~280–320 mOsm, literature) to preserve viability.

• Practical tips:

  • Pre-warm buffers to the chosen incubation temperature to reduce lag time in enzyme activity.
  • Filter-sterilize buffers and use low-retention plastics to reduce protein adsorption.

Item-specific buffer formulation and recommended diluents are Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

Using biological enzymes for tissue dissociation is generally a greener alternative to harsh chemical or high-energy mechanical methods.

• Sustainability perspective (general):

  • Enzymes operate under mild aqueous conditions (near-neutral pH, ambient to physiological temperatures), reducing energy input and hazardous waste compared with strong acids/bases or organic solvents.
  • Enzymatic specificity can decrease over-digestion and improve yields, minimizing repeat processing.

• Comparative overview (general, literature-based):

| Approach | Conditions | Waste profile | Advantages | Trade-offs | |---|---|---|---|---| | Enzymatic dissociation (this product class) | Aqueous buffers, 4–37 °C | Minimal hazardous waste | High viability, specificity; mild | Cost; temperature sensitivity; lot variability | | Mechanical dissociation | Physical shear/agitation | Minimal chemical waste | Solvent-free; simple | Lower yield/viability; epitope damage by shear | | Chemical denaturants (e.g., strong detergents) | Surfactants/chaotropes | Chemical waste; disposal required | Rapid ECM disruption | Poor viability; downstream interference | | Organic solvent-based methods | Solvent mixtures | VOC/hazardous waste | Effective tissue clearing | Not compatible with live cells |

Note: Exact environmental metrics (E-factor, energy use) for this item are Not specified; users can further reduce impact by right-sizing reaction volumes, reusing cold packs, and proper buffer preparation.

Pharmaceutical Uses

This product is supplied strictly for research use only and is not intended for human or veterinary diagnostic or therapeutic applications.

• Formulation/excipient status:

  • No pharmacopeial status or excipient role is provided. Item-specific compliance claims (USP/EP/JP) are Not specified for this item; refer to CoA/Spec Sheet.

• Process development context (general):

  • In R&D settings, enzyme-based dissociation protocols can inform upstream cell isolation workflows for exploratory studies. Transition to GMP-grade materials would be required for clinical manufacturing; this product is not represented as GMP.

• Handling in regulated environments:

  • If using in preclinical research, document lot numbers, storage logs, and in-use stability; assess bioburden/endotoxin and residuals as needed for your internal quality system.

No therapeutic or clinical claims are made or implied for this product.

Physical Properties

Item-specific physical constants are not provided for this enzyme preparation and should be confirmed from the CoA/Spec Sheet.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• State/form: Aqueous protein solution or concentrate is typical for enzyme reagents (literature/general); exact formulation for this item is not specified.
• Concentration/activity units: Not specified for this item; refer to CoA/Spec Sheet.
• pH (as supplied): Not specified for this item; refer to CoA/Spec Sheet.
• Density, refractive index, osmolality: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Enzymes are water-soluble proteins; miscible with common isotonic biological buffers (HBSS, DPBS) in literature practice. Specific diluents for this item are not specified.
• Stability considerations (general, literature-based for protein enzymes):
  • Temperature sensitive; activity decreases on prolonged exposure to >25 °C. Cold-chain storage preserves activity.
  • Freeze–thaw cycles can denature proteins; minimizing cycles is recommended unless the product is explicitly formulated for freezing.
  • Activity profiles typically show dependence on pH and divalent cations; chelators (e.g., EDTA) and serine protease inhibitors can suppress certain enzyme activities.

Note: Where exact numeric values (e.g., protein concentration, activity U/mL, optimal pH, divalent ion requirements) are required for experimental design, consult the item’s CoA/Spec Sheet.

Quality and Grades

• Item-specific grade: EnzymoPure™.

  • EnzymoPure™ is a vendor-specific designation indicating an enzyme preparation intended for research applications. The precise quality criteria (e.g., purity assessment method, activity specification, allowable contaminants, bioburden/endotoxin limits) are Not specified for this item; refer to CoA/Spec Sheet.

• What grade implies (general guidance for enzyme reagents; not item-specific):

  • Defined activity units and lot-to-lot consistency enable reproducible dissociation performance.
  • Tight control of storage/stability parameters to preserve activity during shipping and use.
  • QC may include sterility/bioburden checks, but requirements vary by supplier and intended use.

• Practical notes for professional users:

  • Verify activity units, recommended dilution, and buffer compatibility on the lot-specific CoA before planning digestion times.
  • If downstream applications are sensitive (e.g., scRNA-seq, flow cytometry), request information on nuclease content, endotoxin, and preservative content to ensure compatibility.
  • For comparative studies across tissues, standardize incubation temperature, agitation, and mechanical trituration to minimize variability independent of enzyme grade.

• Appearance, stabilizers, and formulation details: Not specified for this item; refer to CoA/Spec Sheet.

Reaction and Applications

This enzyme preparation is intended for generating single-cell suspensions from solid tissues under gentle, controlled conditions.

• Typical research applications (general, literature-based):

  • Preparation of viable cells for flow cytometry, cell sorting, cell culture, and single-cell omics (scRNA-seq, ATAC-seq).
  • Dissociation of diverse tissues (e.g., tumor, neural, lung, liver, spleen, skin), with protocol adjustments for matrix density and fragility.
  • Reduction of cell clumping by enzymatic degradation of extracellular matrix (ECM) components and cell–cell adhesion molecules.

• Practical considerations:

  • Temperature control: Many protocols operate at 37 °C to balance enzyme activity and cell viability; colder temperatures reduce activity but can protect sensitive epitopes.
  • Mechanical synergy: Gentle trituration or automated dissociation devices complement enzymatic action, shortening incubation and improving yield.
  • DNase supplementation: Free DNA from lysed cells increases viscosity; DNase is commonly included in dissociation workflows in the literature. Confirm compatibility if not provided in the formulation.
  • Quenching/termination: Enzyme activity is often quenched by dilution, cooling, or addition of serum/protein; select an approach aligned with downstream assays.

• Controls and benchmarking:

  • Pilot small-scale digestions to establish time courses that preserve target epitopes and cell-surface markers.
  • Track viability, doublet rate, and marker integrity by flow cytometry; document lot details for reproducibility.

Note: Product-specific tissue recommendations, activity units, and incubation times are Not specified for this item; refer to CoA/Spec Sheet.

Reaction Conditions

Product-specific conditions (temperature, time, and concentration) are not provided and should be obtained from the CoA/Spec Sheet. The following are general, literature-based guidelines for tissue dissociation workflows using enzyme preparations.

• Temperature:

  • Commonly 37 °C for efficient digestion; cold-active protocols (4–10 °C) are used to preserve sensitive epitopes and reduce stress responses.

• Time:

  • Typically 10–60 minutes depending on tissue density and enzyme activity, with intermittent gentle trituration. Monitor aliquots to avoid over-digestion.

• Agitation:

  • Gentle rocking or rotation (e.g., 10–30 rpm) or periodic pipetting with wide-bore tips; avoid vigorous shear that reduces viability.

• Buffer composition:

  • Balanced salts at physiological pH; Ca2+/Mg2+ presence determined by enzyme requirements. DNase may be co-applied to reduce viscosity (literature practice).

• Quench and wash:

  • Dilute with cold buffer, add serum/BSA if appropriate, and perform low-speed centrifugation to collect cells while removing residual enzyme.

• Quality controls:

  • Assess viability (e.g., trypan blue, 7-AAD), doublet rate, and surface marker integrity by flow cytometry; document time–temperature history.

Note: Because activity units and formulation of this specific item are Not specified, perform a small-scale titration to identify minimal effective exposure that preserves viability and marker integrity.

Safety and Handling

Safety data specific to this product are not provided here. Always consult the SDS for authoritative information.

• GHS classification, signal word, H-statements, and pictograms: Not specified for this item; refer to SDS.
• General hazards for protein enzyme preparations (literature/guidance):
  • May cause respiratory or skin sensitization upon repeated exposure to aerosols or powders. Avoid inhalation and contact.
  • Low inherent toxicity is typical, but formulations can contain stabilizers, salts, or preservatives; assess accordingly.
• PPE and engineering controls:
  • Wear lab coat, nitrile gloves, and safety glasses. Handle in a biosafety cabinet when generating single-cell suspensions from primary tissues to control bioaerosols.
  • Use splash protection if vigorous mechanical dissociation is performed.
• Incompatibilities (general):
  • Protease inhibitors, strong chelators (e.g., EDTA/EGTA), extreme pH, oxidants, and elevated temperatures can inactivate enzymes. Verify compatibility with your protocol.
• First-aid overview (general):
  • Skin/eye contact: Rinse with water for at least 15 minutes; remove contaminated clothing; seek medical attention if irritation persists.
  • Inhalation: Move to fresh air; seek medical advice if symptoms occur.
  • Ingestion: Rinse mouth; do not induce vomiting; seek medical attention.
• Biosafety note:
  • When dissociating potentially infectious tissues, follow institutional biosafety level practices and waste decontamination procedures.

Solvent Selection

This product is an aqueous enzyme reagent used in biological buffers rather than in organic solvent systems.

• Applicability:

  • Organic solvent selection frameworks do not apply. Use isotonic, enzyme-compatible buffers.

• Buffer/diluent considerations (general literature guidance):

  • Typical diluents: HBSS, DPBS, or balanced salt solutions with physiological osmolarity to maintain cell viability.
  • pH range: Near-neutral (pH ~7.2–7.6, literature) supports cell viability and many enzyme activities; confirm with product CoA for any specific pH requirements.
  • Divalent cations: Some dissociation enzymes require Ca2+/Mg2+; others are inhibited by EDTA/EGTA. Match buffer composition to the intended activity.
  • Serum/protein supplements: Albumin or serum can protect cells but may reduce enzyme activity; empirical optimization is common.

• When to choose this product vs alternatives (general):

  • Use an all-in-one tissue dissociation enzyme when you require broad applicability across tissue types and wish to minimize optimization compared to custom cocktails.
  • Choose specialized enzymes (e.g., purified collagenase isoforms, elastase, dispase, DNase I) when tissue matrix composition or downstream assays demand precise control of cleavage specificity.

Note: Specific diluent recommendations for this item are Not specified; consult the CoA/Spec Sheet.

Storage and Reconstitution

• Storage conditions (item-specific): Store at 2–8 °C.

• Shipping (item-specific): Shipped on wet ice.

• Container handling:

  • Keep tightly closed. Minimize time at ambient temperature during handling. Return to 2–8 °C promptly after use.

• Stability and in-use guidance (general for enzyme reagents; item-specific data not provided):

  • Avoid repeated freeze–thaw cycles unless the formulation is validated for freezing; aliquot if freezing is necessary per your workflow.
  • Protect from microbial contamination; use sterile technique and, if appropriate, sterile-filtered buffers.
  • Do not store diluted working solutions for extended periods unless stability is documented; prepare fresh immediately before use.

• Reconstitution:

  • If supplied as a liquid, use as provided. If supplied as a concentrate or lyophilizate, follow the lot-specific instructions. Item-specific reconstitution buffer, volume, and activity recovery are Not specified for this item; refer to CoA/Spec Sheet.

• Research use note: For research use only; not for use in diagnostic procedures.

Structure and Identity

This product is a protein-based enzymatic preparation formulated for dissociating intact tissues into single-cell suspensions. Item-specific structural identifiers (small-molecule style) are not applicable.

• Item-specific identifiers:

  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • SMILES: Not applicable to protein mixtures.
  • InChIKey: Not applicable to protein mixtures.
  • CAS Registry Number: Not specified for this item; refer to CoA/Spec Sheet.
  • EC/CAS names: Not specified for this item; refer to CoA/Spec Sheet.

• Structural features (general, literature-based for protein enzymes):

  • Proteins composed of amino acids with peptide backbones; may include domains for substrate binding and catalysis (e.g., proteolytic, carbohydrate-active, DNase/RNase activities depending on formulation). Specific composition for this product is proprietary/not specified.
  • Usually globular tertiary structures stabilized by noncovalent interactions; activity is sensitive to temperature, pH, and ionic strength.

• Product type and intended use:

  • Category: Protein/enzyme reagent for tissue dissociation into single cells for downstream cell biology workflows (flow cytometry, single-cell omics, primary culture), research use only.
  • Grade: EnzymoPure™ (vendor-specific grade; see Quality & Grades).

• 2D/functional description in words (general):

  • Aqueous enzyme preparation intended to hydrolyze extracellular matrix components and cell–cell junctions under controlled conditions to release viable single cells from solid tissues.

Synthetic Utility

Not typically applicable. This is a biological enzyme preparation for cell isolation, not a small-molecule building block or reagent in chemical synthesis.

• Relevance for chemists (general):
  • While not used in synthetic organic chemistry, the reagent enables preparation of primary cells for biocatalysis screening, cell-based assays, or single-cell analytics that can inform medicinal chemistry and drug discovery.

For chemical transformation-focused guidance, refer to other reagents designed for synthetic reactions.

Target Specificity

No antigen, clone, or molecular target specificity is specified for this product. It is a general tissue dissociation enzyme preparation rather than a target-specific biologic.

• Item-specific target data: Not specified for this item; refer to CoA/Spec Sheet.
• General note: Enzymatic activity in tissue dissociation typically acts on extracellular matrix proteins and cell adhesion structures; exact substrate spectrum depends on the proprietary formulation (not provided).

Perguntas frequentes

What is EnzymoPure??

EnzymoPure? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, EnzymoPure? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.