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3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System
AVAILABLE TO ORDER
GRADE & PURITY peroxidase substrate
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Why this grade
peroxidase substrate for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Literature proof
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
Specifications
Product Name
3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System
Specifications & Purity
peroxidase substrate
Molecule Type
Bioreagents/Buffer
Storage and Shipping
Concentration
peroxidase substrate
Storage
Store at 2-8°C
Shipped In
Wet ice
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →✅ Certificate of Analysis (COA)
Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →🔬 Specification Sheet
Full quality attributes and acceptance criteria for this grade.
View spec sheet →Advanced Data
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Documents & Articles
Citations of This Product
References
| 1. Yingxue Li, Tingrui Zhang, Gang Bai, Mengchun Chen, Xiaodong Lei, Li Ye, Hua Yu, Zhichao Fan, Tao Yu. (2024) A target-triggered colorimetric sensor for ultrasensitive detection of miRNAs based on self-powered three-dimensional DNA walker. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:39265909] [10.1016/j.ijbiomac.2024.135370] |
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