AF647-NHS ester triTEA (5C-Linker) - BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥98%(HPLC) , CAS No.1620475-28-6(free base)

CAS: 1620475-28-6(free base) Cat. No.: A1454668 Fórmula: C₃₉H₄₇N₃O₁₆S₄ (free base) Peso molecular: 942.06 (free base)

GRADE & PURITY BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥98%(HPLC)

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

Cell Staining, Fluorescence Analysis, Protein/Antibody Labeling

Descripción general

The NHS ester moiety in AF647-NHS ester (5C-Linker) is a commonly used amine-reactive group. It activates the carboxyl group on the AF647 molecule, enabling it to react with amino groups (primarily primary amines) on target biomolecules such as proteins, antibodies and peptides. This reaction forms stable amide bonds to visualize and label biomolecules. It is widely applied in flow cytometry, confocal microscopy and fluorescence-based assays for labeling and detection of cells, proteins and other biological entities. It can also serve as a sensitive reporter group in immunoassays. Ex/Em(nm)=651/671.

Spectral properties: 

Excitation/absorption maximum: 651 nm 

Emission maximum: 671 nm 

Extinction coefficient (ε): 265000 

Fluorescence quantum yield (Φ): 0.33 

Precautions for Fluorescent Products: 

1. Activated esters are prone to hydrolysis. After taking out from the refrigerator, allow the vial to warm to room temperature in a dry environment before opening. Since the reagent quantity is small, gently tap the vial several times before opening to ensure all powder falls to the bottom.

2. It is recommended to prepare the stock solution fresh at first use. Use the dissolved reagent as soon as possible to guarantee experimental performance.

3. Fresh anhydrous DMSO is required for dissolution. Water contamination will cause hydrolysis of the activated ester and compromise experimental results.

4. The prepared stock solution is susceptible to hydrolysis upon exposure to water. If not fully consumed in one experiment, aliquot the solution (e.g., 5–8 μL per tube), seal with parafilm, wrap with aluminum foil, place in a well-sealed plastic bag containing desiccant, and store hermetically at ≤ -20°C protected from light.

5. It is recommended to optimize cell density, dye/probe concentration and incubation time in preliminary tests before formal experiments to determine optimal experimental conditions.

Specifications

Especificaciones y pureza

BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥98%(HPLC)

Estabilidad y almacenamiento

Store at -20℃ long term (24 months). Store in the dark. Desiccated. Avoid freeze/thaw cycle.

Condiciones de almacenamiento de almacenamiento

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

Biological Stain, BioReagent, para el análisis de fluorescencia, para microscopía

Pureza

≥98%(HPLC)

Nombres e identificadores

• Peso molecular: 942.06 (free base)

Propiedades químicas y físicas

• Solubilidad: Soluble in DMSO.

Preguntas frecuentes

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 407627-60-5, the molecular formula is C40H49N3O16S4, and the molecular weight is 956.09 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.