Aurora A Mouse mAb - Anticuerpo primario, specific to Human AURKA

Cat. No.: Ab184689

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, IF/ICC, WB

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG2b

Clone Number

EF01/3C12

Conjugation

Unconjugated

Descripción general

Mouse anti Human AURKA Antibody, clone EF01/3C12, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Specifications

Product Name

Aurora A Mouse mAb - Anticuerpo primario, specific to Human AURKA

Sinónimos

AIK antibody | ARK-1 antibody | ARK1 antibody | AURA antibody | Aurka antibody | Aurora 2 antibody | Aurora A antibody | Aurora kinase A antibody | Aurora-related kinase 1 antibody | Aurora/IPL1 like kinase antibody | AURORA/IPL1-like kinase antibody | Au

Especificaciones y pureza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Mecanismos bioquímicos y fisiológicos

Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mit

Especies hospedadoras

Ratón

Especificidad

Human AURKA

Inmunógeno

Recombinant protein corresponding to Human AURKA

Control positivo

WB: MCF-7 whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150.0 KDa

Método de purificación

Protein A purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imágenes

Western Blot: Aurora A Mouse mAb (Ab184689)

Aurora A Mouse mAb (Ab184689) - Western Blot
All lanes: Aurora A Mouse Antibody (Ab184689) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 46 kDa
Observed band size: 42 kDa
Exposure time: 37.3 s 

Flow Cytometry: Aurora A Mouse mAb (Ab184689)

Aurora A Mouse mAb (Ab184689) - Flow Cytometry ​
Flow Cytometry analysis of HeLa cells stained with Aurora A  Mouse mAb (Ab184689) (Red) at 0.25 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue). ​

IF/ICC: Aurora A Mouse mAb (Ab184689)

Aurora A Mouse mAb (Ab184689) - IF/ICC
IF analysis of Aurora A (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Aurora A Mouse mAb (Ab184689) at 2.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Aplicación

• Aplicación: Dilution info
• WB:

  1/1000 - 1/2000

• IF/ICC:

  1/200;

• Flow Cytometry:

  1/1000 - 1/10000

Objetivos asociados (humanos)

AURKA Tchem Aurora kinase A (0 Activities)

Discover Target: AURKA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Aurora A Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.