BIS-TRIS - for cell culture, suitable for insect cell culture, ≥98%(HPLC) , CAS No.6976-37-0

CAS: 6976-37-0 Cat. No.: B113072 Molecular Weight: 209.24 Beilstein Registry Number: 2205275 EC Number: 686-880-2

GRADE & PURITY for Cell culture, Suitable for insect cell culture, ≥98%(HPLC)

Synonyms

BIS-TRIS | 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol | BIS-TRIS BUFFER | Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane | 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol | Bistris

Storage

Room temperature,Argon charged

Shipped In

Normal

Overview

BIS-Tris is an inert, zwitterionic buffer that exhibits only a very small shift in dissociation constant with respect to temperature. Suitable for fractionation of hemoglobin. The useful pH range for the buffer is 5.8-7.2. Has a pKa of 6.5 at 25°C.
An inert, zwitterionic buffer that exhibits only a very small shift in dissociation constant.

Specifications

Synonyms

BIS-TRIS | 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1, 3-diol | BIS-TRIS BUFFER | Bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane | 2, 2-Bis(hydroxymethyl)-2, 2', 2''-nitrilotriethanol | Bistris

Specifications & Purity

for cell culture, suitable for insect cell culture, ≥98%(HPLC)

Storage

Room temperature, Argon charged

Shipped In

Normal

Grade

for Cell culture, Suitable for insect cell culture

Purity

≥98%(HPLC)

Names and Identifiers

• Pubchem Sid: 504755532
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504755532
• Canonical Smiles: C(CO)N(CCO)C(CO)(CO)CO
• IUPAC Name: 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol
• InChIKey: OWMVSZAMULFTJU-UHFFFAOYSA-N
• INCHI: 1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2
• Isomeric SMILES: C(CO)N(CCO)C(CO)(CO)CO
• WGK Germany: 3
• Molecular Weight: 209.24
• Beilstein: 2205275
• Reaxy-Rn: 2205275
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=2205275&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic nitrogen compounds
    • Class Organonitrogen compounds
      • Subclass Amines
        • Level5 Alkanolamines
          • Level6 1,2-aminoalcohols

• Kingdom: Organic compounds
• Superclass: Organic nitrogen compounds
• Class: Organonitrogen compounds
• Subclass: Amines
• Intermediate Tree Nodes: Alkanolamines
• Direct Parent: 1,2-aminoalcohols
• Alternative Parents: Trialkylamines Primary alcohols Organopnictogen compounds Hydrocarbon derivatives
• Molecular Framework: Aliphatic acyclic compounds
• Substituents: Tertiary aliphatic amine - Tertiary amine - 1,2-aminoalcohol - Organic oxygen compound - Organopnictogen compound - Hydrocarbon derivative - Primary alcohol - Organooxygen compound - Alcohol - Aliphatic acyclic compound
• Description: This compound belongs to the class of organic compounds known as 1,2-aminoalcohols. These are organic compounds containing an alkyl chain with an amine group bound to the C1 atom and an alcohol group bound to the C2 atom.
• External Descriptors: pentol

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Soluble in water (~209.2 g/l) at 20 °C, and alcohol.
• Sensitivity: Moisture sensitive
• Melt Point(°C): 103-104°C
• Molecular Weight: 209.240 g/mol
• XLogP3: -3.300
• Hydrogen Bond Donor Count: 5
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 8
• Exact Mass: 209.126 Da
• Monoisotopic Mass: 209.126 Da
• Topological Polar Surface Area: 104.000 Ų
• Heavy Atom Count: 14
• Formal Charge: 0
• Complexity: 125.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

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