Carboxypeptidase B, CAS No.9025-24-5

CAS: 9025-24-5 Cat. No.: C1492282 Molecular Weight: 610.7 EC Number: 232-788-9 PubChem CID: 3289209

GRADE & PURITY Animal Free, Carrier Free, Bioactive, Recombinant, ActiBioPure™, EnzymoPure™, ≥90%(SDS-PAGE), ≥150 U/mg protein, expressed in Pichia pastoris

Accession #

P09955

Expression system

Pichia pastoris

Bioactivity

≥150 U/mg protein

Overview

Carboxypeptidase B, also known as peptidyl - L - lysine (L - arginine) hydrolase and arginase, is a metalloprotease that can specifically hydrolyze basic amino acids (lysine, arginine, histidine) at the C - terminus of proteins.
This product is a recombinant carboxypeptidase B lyophilized powder obtained by isolation and purification from recombinant Pichia pastoris, followed by freeze - drying. It is a biochemical - grade genetic engineering enzyme, free from exogenous virus contamination, and does not contain enzyme inhibitors such as DFP, PMSF, and TLCK. It has the same activity and specificity as natural carboxypeptidase B.
Product Advantages
Animal - free origin: No animal viruses, no pathogenic substances, no exogenous factor contamination, and high safety.
High purity and high specific activity.
Production scale is over 1000L.
Compliance: Production equipment and production environment meet relevant regulatory requirements and conform to GMP guidelines.
Complete quality documents: Relevant regulatory support documents can be provided according to customer needs.
Product Characteristics
Appearance: White or off - white powder
Enzyme specific activity: ≥150 U/mg protein
Molecular weight: 36kD ± 3.6kD
Purity: ≥90% (SDS - PAGE)
Protein content: ≥30%
Optimal pH: 7.0 - 9.0
Solubility: Soluble in buffer solution
Recommended Usage Methods
Preparation of protein
Dissolve the protein in a 25mM Tris - HCl solution with pH 7.6.
Preparation of enzyme solution
Weigh the required enzyme powder and dissolve it in 25mM Tris - HCl (pH 7.6) to make the enzyme solution concentration 1 - 10mg/ml.
Reaction
Recommended enzyme amount: Target protein (mass ratio) = 1:50 - 1:1000, and the optimal pH is 7.0 - 9.0.
Product Applications
Production of various proteins or polypeptides, such as insulin and its analogs, beauty peptides, antimicrobial peptides, food flavor peptides, etc.
Quality detection of antibodies.
Determination of amino acids at the C - terminus of proteins.
Storage stability
The lyophilized powder can be stably stored for at least 24 months at -15°C or below. After being dissolved in 25 mM Tris-HCl (pH 7.6) and stored at -15°C or below, there is no loss of activity even after 10 cycles of repeated freezing and thawing. It is recommended that the dissolved product be stored at 2–8°C for no more than 7 days, and at -15°C or below for no more than 30 days.
Precautions and Disclaimer
Its activity is competitively inhibited by arginine and lysine; metal ion chelators (such as EDTA) also have an inhibitory effect on the enzyme activity.
This product is hygroscopic and should be equilibrated at room temperature before being taken and used.
This product is for research use only and shall not be used for the diagnosis or treatment of animals or humans.

Specifications

Product Name

Carboxypeptidase B, CAS No.9025-24-5

Synonyms

protaminase | CPB1

Grade

ActiBioPure™, Animal Free, Bioactive, Carrier Free, Recombinant, EnzymoPure™

Specifications & Purity

Animal Free, Carrier Free, Bioactive, Recombinant, ActiBioPure™, EnzymoPure™, ≥90%(SDS-PAGE), ≥150 U/mg protein, expressed in Pichia pastoris

Biochemical and Physiological Mechanisms

Preferential release of a C-terminal lysine or arginine amino acid.

Bioactivity

≥150 U/mg protein

Accession #

P09955

Predicted molecular weight

36 kDa

CAS

9025-24-5

Enzyme Commission Number

3.4.17.2

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥150 U/mg protein, expressed in Pichia pastoris

Reconstitution

Reconstitute in 25 mM Tris-HCl, pH 7.6 to a concentration of 1-10 mg/ml.

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (2 years). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Unit definition

The amount of enzyme that catalyzes the hydrolysis of 1 μmol of hippuric acid - L - arginine in 1 minute under the conditions of 25°C and pH 7.65 is 1 U.

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Peptides
          • Level6 Oligopeptides

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Peptides
• Direct Parent: Oligopeptides
• Alternative Parents: Phenylalanine and derivatives N-acyl-alpha amino acids and derivatives Proline and derivatives Alpha amino acid amides Alanine and derivatives Amphetamines and derivatives N-acylpyrrolidines Pyrrolidinecarboxamides N-acyl amines Tertiary carboxylic acid amides Thioesters Secondary carboxylic acid amides Carbothioic S-esters Monocarboxylic acids and derivatives Carboxylic acids Azacyclic compounds Sulfenyl compounds Organonitrogen compounds Organic oxides Hydrocarbon derivatives Carbonyl compounds Organopnictogen compounds
• Molecular Framework: Aromatic heteromonocyclic compounds
• Substituents: Alpha-oligopeptide - Phenylalanine or derivatives - Proline or derivatives - N-acyl-alpha amino acid or derivatives - Alpha-amino acid amide - Alanine or derivatives - N-substituted-alpha-amino acid - Alpha-amino acid or derivatives - Amphetamine or derivatives - N-acylpyrrolidine - Pyrrolidine carboxylic acid or derivatives - Pyrrolidine-2-carboxamide - Monocyclic benzene moiety - Fatty amide - N-acyl-amine - Fatty acyl - Benzenoid - Tertiary carboxylic acid amide - Pyrrolidine - Secondary carboxylic acid amide - Carbothioic s-ester - Thiocarboxylic acid ester - Carboxamide group - Organoheterocyclic compound - Sulfenyl compound - Thiocarboxylic acid or derivatives - Azacycle - Carboxylic acid - Monocarboxylic acid or derivatives - Organic oxide - Organic oxygen compound - Organopnictogen compound - Hydrocarbon derivative - Carbonyl group - Organic nitrogen compound - Organonitrogen compound - Organooxygen compound - Organosulfur compound - Aromatic heteromonocyclic compound
• Description: This compound belongs to the class of organic compounds known as oligopeptides. These are organic compounds containing a sequence of between three and ten alpha-amino acids joined by peptide bonds.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y