Cathepsin E Antibody - Anticuerpo primario, Rabbit IgG

Cat. No.: Ab093172

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Conejo

Reactive species

consejo, Humano, Ratón

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human Cathepsin E Antibody, Polyclonal, could be used for WB, IHC and so on.

Application:

WB: 1/500-1/2000

IHC: 1/100-1/300

Protein Function

May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.

Post-translational

Glycosylated. The nature of the carbohydrate chain varies between cell types. In fibroblasts, the proenzyme contains a high mannose-type oligosaccharide, while the mature enzyme contains a complex-type oligosaccharide. In erythrocyte membranes, both the proenzyme and mature enzyme contain a complex-type oligosaccharide. Two forms are produced by autocatalytic cleavage, form I begins at Ile-54, form II begins at Thr-57.

Specifications

Product Name

Cathepsin E Antibody - Anticuerpo primario, Rabbit IgG

Sinónimos

CATE antibody | CATE_HUMAN antibody | Cathepsin E antibody | Cathepsin E form II antibody | CTSE antibody | Erythrocyte membrane aspartic proteinaseantibody | Slow moving proteinase antibody1510CATE | Cathepsin E | CTSE | EC 3.4.23 | EC 3.4.23.34 | erythr

Especificaciones y pureza

Validated, ExactAb™, 1.0 mg/mL

Especies hospedadoras

Conejo

Inmunógeno

A synthetic peptide derived from human Cathepsin E (AA 243-292)

Control positivo

WB: Mouse liver, Mouse stomach, Rat liver lysates. IHC: Human lung carcinoma tissue. IF/ICC: HeLa cells.

Conjugación

Unconjugated

Grado

ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Immunogen affinity purified

Concentración

1.0 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Imágenes

Western Blot: Cathepsin E Antibody (Ab093172)

Cathepsin E Antibody (Ab093172) - Western Blot
All lanes: Cathepsin E Antibody (Ab093172) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab176443) at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 51 kDa
Exposure time: 28.0 s

IHC: Cathepsin E Antibody (Ab093172)

Cathepsin E Antibody (Ab093172) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma tissue using Cathepsin E Antibody (Ab093172). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Cathepsin E Antibody (Ab093172)

Cathepsin E Antibody (Ab093172) - IF/ICC
IF analysis of Cathepsin E Antibody (Ab093172) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Cathepsin E Antibody (Ab093172) at 1/50 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Aplicación

• Aplicación: Dilution info
• IHC:

  1/100-1/300

• WB:

  1/500-1/2000 ;

• IF/ICC:

  1/50 ;

Objetivos asociados (humanos)

CTSE Tchem Cathepsin E (0 Activities)

Discover Target: CTSE

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Cathepsin E Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.