CALIDAD & PUREZACarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Validated, High Performance, See COA Carrier Free,ExactAb™,High Performance,Validated para flujos de trabajo cromatográficos y analíticos sensibles que requieren una interferencia mínima de la línea base.
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Almacenamiento y envío
Store at -20°C,Avoid repeated freezing and thawing Envío Ice chest + Ice pads. Consulte el COA específico del lote para conocer las especificaciones exactas.
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Documentos de calidad
SDS, COA, ficha técnica y hoja de especificaciones disponibles para descargar. COA específico del lote accesible mediante búsqueda por número de lote.
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Prueba documental
Citado en 0 publicaciones revisadas por pares sobre cromatografía, síntesis orgánica y reacciones de acoplamiento cruzado.
Descripción general
Mouse anti Human CD3 epsilon Antibody, Monoclonal (C10), could be used for WB, IHC and so on. Application: WB: 0.01-3µg/mL IHC: 5-20µg/mL Protein Function: The CD3 complex mediates signal transduction.
Especificaciones
Product Name
CD3 epsilon Mouse mAb - Anticuerpo primario, specific to CD3E, Mouse IgG1
Carrier Free, ExactAb™, Validated, High Performance, See COA
Especies hospedadoras
Ratón
Especificidad
CD3E
Inmunógeno
Recombinant CD3e expressed in E.coli (AA 28-185)
Control positivo
WB: Jurkat and MOLT-4 cell lysates. IHC: Human spleen tissue.
Conjugación
Unconjugated
Grado
Carrier Free, ExactAb™, High Performance, Validated
Isotipo
Mouse IgG1
Tipo de cadena ligera
kappa
SDS-PAGE
150 kDa
Método de purificación
Protein A + Protein G purified
Concentración
See COA
Product Line
Productos biológicos
Almacenamiento y envío
Forma
Liquid
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
CD3 epsilon Mouse mAb (Ab094446) - Western Blot All lanes: CD3 epsilon Mouse mAb (Ab094446) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 23 kDa Observed band size: 22 kDa Exposure time: 20.0 s
CD3 epsilon Mouse mAb (Ab094446) - IHC DAB staining on IHC Sample: Human Spleen Tissue Primary Ab: 20 µg/mL CD3 epsilon Mouse mAb (Ab094446) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Aplicación
Aplicación
Dilution info
WB
0.01-3 µg/mL
IHC
5-20 µg/mL
Documentación
📋 Hoja de datos de seguridad (SDS)
Documento completo sobre peligros, manipulación, almacenamiento y cumplimiento normativo.
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Dilution Calculator
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Reconstitution Calculator
Reseñas
Reseñas de cliente
Preguntas frecuentes
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD3 epsilon Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
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