CD4 Mouse mAb (FITC) - Anticuerpo primario, specific to CD4

Cat. No.: Ab210101

GRADE & PURITY Validated, ExactAb™, Ex:498nm, Em:517nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

Ratón

Reactive species

consejo

Isotype

Mouse IgG2a

Clone Number

OX-38

Conjugation

FITC

Descripción general

Mouse anti Rat CD4 Antibody, Monoclonal (OX-38), could be used for Flow.

Specifications

Product Name

CD4 Mouse mAb (FITC) - Anticuerpo primario, specific to CD4

Sinónimos

p55 | W3/25 | CD4 | CD4 antigen (p55) | CD4 antigen | CD4 molecule | CD4 receptor | CD4mut | T-cell surface antigen T4/Leu-3 | T-cell surface glycoprotein CD4 | CD 4 antibody | CD4 (L3T4) antibody | CD4 antibody | CD4 antigen (p55) antibody | CD4 antigen

Especificaciones y pureza

ExactAb™, Validated, Ex:498nm, Em:517nm, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

Especies hospedadoras

Ratón

Especificidad

CD4

Inmunógeno

Rat thymocyte glycoproteins

Control positivo

Flow: rat splenocytes

Conjugación

FITC

Excitación (nm)

Blue Laser(488nm)

Emisión (nm)

517nm

Ex/Em(nm)

Ex:498nm;Em:517nm

Grado

Em:517nm, Ex:498nm, ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein A purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: CD4 Mouse mAb (FITC) (Ab210101)

CD4 Mouse mAb (FITC) (Ab210101) - Flow Cytometry
Flow cytometry analysis of CD4 expression by rat splenocytes cells (right panel) compared to an isotype control (left panel). Cells were stained with CD4 Mouse mAb (FITC) (Ab210101) at 0.1μg/test for 1 hour at 4°C. Left panel – Mouse IgG (FITC) (Ab180307).

Flow Cytometry: CD4 Mouse mAb (FITC) (Ab210101)

CD4 Mouse mAb (FITC) (Ab210101) - Flow Cytometry
Flow cytometry analysis of CD4 expression by rat splenocytes cells (red) compared to an isotype control (blue). Cells were stained with CD4 Mouse mAb (FITC) (Ab210101) at 0.1μg/test for 1 hour at 4°C. Blue - Mouse IgG (FITC) (Ab180307). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤1.0 μg/test, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume ;

Objetivos asociados (humanos)

CD4 Tclin T-cell surface glycoprotein CD4 (0 Activities)

Discover Target: CD4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD4 Mouse mAb (FITC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.