CD40 Mouse mAb - Anticuerpo primario, specific to CD40, Mouse IgG1

Cat. No.: Ab094764

GRADE & PURITY Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Flow Cytometry, Functional Assay, Stimulation

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

G28.5

Conjugation

Unconjugated

Descripción general

Mouse anti Human CD40 Antibody, Monoclonal (G28.5), could be used for in vitro CD40 stimulation, Functional assays, Flow and so on.
Application:
in vitro CD40 stimulation: Use at an assay dependent concentration.
Functional assays: Use at an assay dependent concentration.
Flow: Use at an assay dependent concentration.
Protein Function
Receptor for TNFSF5/CD40LG.

Specifications

Product Name

CD40 Mouse mAb - Anticuerpo primario, specific to CD40, Mouse IgG1

Sinónimos

Bp50 | CDW40 | B cell surface antigen CD40 | B-cell surface antigen CD40 | Bp50B cell-associated molecule | CD40 antigen | CD40 molecule, TNF receptor superfamily member 5 | CD40 type II isoform | CD40 | CD40L receptor | CDw40 | MGC9013 | nerve growth fac

Especificaciones y pureza

Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Especies hospedadoras

Ratón

Especificidad

CD40

Inmunógeno

Human tonsillar lymphocytes

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, PBS Only, Validated

Isotipo

Mouse IgG1

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Imágenes

Flow Cytometry: CD40 Mouse mAb (Ab094764)

CD40 Mouse mAb (Ab094764) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD40 with CD40 Mouse mAb (Ab094764) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD40 Mouse mAb (Ab094764)

CD40 Mouse mAb (Ab094764) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD40 (red) with CD40 Mouse mAb (Ab094764) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

ELISA: CD40 Mouse mAb (Ab094764)

CD40 Mouse mAb (Ab094764) - ELISA
Immobilized Recombinant Human CD40/TNFRSF5 protein (rp183577) at 1.0 μg/mL can bind CD40 Mouse mAb (Ab094764) with the EC₅₀ of 15.35 ng/mL.

Aplicación

Aplicación

Dilution info

Functional Assay

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vitro CD40 stimulation

Use at an assay dependent concentration.

Objetivos asociados (humanos)

CD40 Tchem Tumor necrosis factor receptor superfamily member 5 (0 Activities)

Discover Target: CD40

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD40 Tchem Tumor necrosis factor receptor superfamily member 5 (9 Activities)

Discover Target: CD40

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de acción

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD40 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.