CD45.2 Mouse mAb - Anticuerpo primario, specific to CD45.2, Mouse IgG2a

Cat. No.: Ab094926

CALIDAD & PUREZA Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, IHC

Host species

Ratón

Reactive species

Ratón

Isotype

Mouse IgG2a

Clone Number

104.2

Conjugation

Unconjugated

Descripción general

Mouse anti Mouse CD45.2 Antibody, Monoclonal (104.2), could be used for Flow, in vivo CD45.2 blockade, in vitro CD45.2 blockade, IHC and so on.
Application:
Flow: Use at an assay dependent concentration.
in vivo CD45.2 blockade: Use at an assay dependent concentration.
in vitro CD45.2 blockade: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
Protein Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

Especificaciones

Product Name

CD45.2 Mouse mAb - Anticuerpo primario, specific to CD45.2, Mouse IgG2a

Sinónimos

loc | Cd45 | Ly-5 | Lyt-4 | B220 | CD_antigen: CD45 | CD45 antigen | CD45R | EC 3.1.3.48 | EC:3.1.3.48 | GP180 | LCA | L-CA | Leukocyte common antigen | LY5 | protein tyrosine phosphatase, receptor type, c polypeptide | PTPRC | receptor-type tyrosine-prot

Especificaciones y pureza

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Especies hospedadoras

Ratón

Especificidad

CD45.2

Inmunógeno

B10.S mouse thymocytes and splenocytes

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotipo

Mouse IgG2a

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Imágenes

Flow Cytometry: CD45.2 Mouse mAb (Ab094926)

CD45.2 Mouse mAb (Ab094926) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD45.2 with CD45.2 Mouse mAb (Ab094926) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD45.2 Mouse mAb (Ab094926)

CD45.2 Mouse mAb (Ab094926) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD45.2 (red) with CD45.2 Mouse mAb (Ab094926) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

Aplicación

Dilution info

IHC

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vivo CD45.2 blockade

Use at an assay dependent concentration.

in vitro CD45.2 blockade

Use at an assay dependent concentration.

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD45.2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.