CD70 Rat mAb - Anticuerpo primario, specific to CD70, Rat IgG2b

Cat. No.: Ab095232

CALIDAD & PUREZA Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay

Host species

consejo

Reactive species

Ratón

Isotype

Rat IgG2b

Clone Number

FR70

Conjugation

Unconjugated

Descripción general

Rat anti Mouse CD70 Antibody, Monoclonal (FR70), could be used for Flow, in vivo CD70 blockade, in vitro CD70 blockade and so on.
Application:
Flow: Use at an assay dependent concentration.
in vivo CD70 blockade: Use at an assay dependent concentration.
in vitro CD70 blockade: Use at an assay dependent concentration.
Protein Function
Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.

Especificaciones

Product Name

CD70 Rat mAb - Anticuerpo primario, specific to CD70, Rat IgG2b

Sinónimos

Cd27l | Tnfsf7 | Tnlg8a | CD27 Ligand | CD27-L | CD27LCD27 ligand | CD27LG | CD70 molecule | CD70 | Ki-24 antigen | surface antigen CD70 | TNFSF7 | TNFSF7CD70 antigen | tumor necrosis factor (ligand) superfamily, member 7 | Tumor necrosis factor ligand su

Especificaciones y pureza

Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Especies hospedadoras

consejo

Especificidad

CD70

Inmunógeno

BALB/c mouse B lymphoma A20.2J

Control positivo

IHC-Fr: Human tonsil, and human kidney tissue. Flow Cyt: Raji cells.

Conjugación

Unconjugated

Grado

Azide Free, Carrier Free, PBS Only, Validated

Isotipo

Rat IgG2b

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

See COA

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Do not freeze

Enviado en

Wet ice,Do not freeze

Imágenes

Flow Cytometry: CD70 Rat mAb (Ab095232)

CD70 Rat mAb (Ab095232) - Flow Cytometry
Flow Cytometry analysis of 10 μg/mL LPS stimulated 3 days mouse splenocytes cells labelling CD70 with CD70 Rat mAb (Ab095232) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD70 Rat mAb (Ab095232)

CD70 Rat mAb (Ab095232) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD70 (red) with CD70 Rat mAb (Ab095232) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

Aplicación

Dilution info

Flow Cytometry

Use at an assay dependent concentration.

in vivo CD70 blockade

Use at an assay dependent concentration.

in vitro CD70 blockade

Use at an assay dependent concentration.

Objetivos asociados (humanos)

CD70 Tbio CD70 antigen (0 Activities)

Discover Target: CD70

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD70 Rat mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.