CD86 Rat mAb (AF647) - Primary antibody, specific to CD86

Cat. No.: Ab215324

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

GL1

Conjugation

AF647

Overview

Rat anti Mouse CD86 Antibody, Monoclonal (GL1), could be used for Flow Cytometry.

Specifications

Product Name

CD86 Rat mAb (AF647) - Primary antibody, specific to CD86

Synonyms

Activation B7-2 antigen | B70 | B7-2 antigen | B72 | B7-2 | B-lymphocyte activation antigen B7-2 | BU63 | CD28 antigen ligand 2 | CD28LG2B7-2 antigen) | CD86 antigen | CD86 molecule | CD86 | CTLA-4 counter-receptor B7.2 | FUN-1 | LAB72 | MGC34413 | T-lymp

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as d

Host species

Rat

Specificity

CD86

Immunogen

LPS-activated CBA/Ca mouse splenic B cells

Positive Control

Flow: C57BL/6 mouse splenocytes

Conjugation

AF647

Excitation (nm)

Red Laser(633nm)

Emission(nm)

668nm

Ex/Em(nm)

Ex:650nm;Em:668nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD86 Rat mAb (AF647) (Ab215324)

CD86 Rat mAb (AF647) (Ab215324) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) stained with CD86 Rat mAb (AF647) (Ab215324) (red) compared to an isotype control (blue). Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).

Flow Cytometry: CD86 Rat mAb (AF647) (Ab215324)

CD86 Rat mAb (AF647) (Ab215324) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) stained with CD86 Rat mAb (AF647) (Ab215324) (right panel) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF647) (Ab190071).

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD86 Tclin T-lymphocyte activation antigen CD86 (0 Activities)

Discover Target: CD86

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)