CHAPS hydrate - ≥98%(HPLC) , CAS No.331717-45-4

CAS: 331717-45-4 Cat. No.: C464942 Fórmula: C₃₂H₅₈N₂O₇S · xH₂O Peso molecular: 614.88 (anhydrous basis) Número EC: 695-474-4

GRADE & PURITY ≥98%(HPLC)

Synonyms

SCHEMBL1096579 | CHAPS (hydrate) | 3-[dimethyl-[3-[[(4R)-4-[(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-3,7,12-trihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoyl]amino]propyl]azaniumyl]propane-1

Storage

Room temperature

Descripción general

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.A solution at 50 mg/ml in water yields a clear-colorless solution. Aqueous solutions of CHAPS can be autoclaved.0.2 w/v of CHAPS has been used to remove cerebral endothelium by brain perfusion. 2% (w/v) CHAPS has been used as rehydration buffer along with Thiourea and Urea in protein extraction. 10 mM CHAPS has been used along with 0.15 M NaCl, Tris–Cl, pH 7.6, EDTA as lysis buffer in Western Blot analysis. CHAPS has been used as hybridization buffer in in-situ hybridization method of immature mammalian CNS.

Specifications

Sinónimos

SCHEMBL1096579 | CHAPS (hydrate) | 3-[dimethyl-[3-[[(4R)-4-[(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-3,7,12-trihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoyl]amino]propyl]azaniumyl]propane-1

Especificaciones y pureza

≥98%(HPLC)

Condiciones de almacenamiento de almacenamiento

Room temperature

Pureza

≥98%(HPLC)

Nombres e identificadores

• Pubchem Sid: 504770519
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504770519
• Sonrisas canónicas: CC(CCC(=O)NCCC[N+](C)(C)CCCS(=O)(=O)[O-])C1CCC2C1(C(CC3C2C(CC4C3(CCC(C4)O)C)O)O)C.O
• IUPAC Name: 3-[dimethyl-[3-[[(4R)-4-[(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-3,7,12-trihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoyl]amino]propyl]azaniumyl]propane-1-sulfonate;hydrate
• InChIKey: SJCUTFKCLFLIFE-JWTJKVBLSA-N
• INCHI: 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
• Isómeros SMILES: C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS(=O)(=O)[O-])[C@H]1CC[C@@H]2[C@@]1([C@H](C[C@H]3[C@H]2[C@@H](C[C@H]4[C@@]3(CC[C@H](C4)O)C)O)O)C.O
• WGK Alemania: 1
• CAS alternativo: 75621-03-3
• Peso molecular: 614.88 (anhydrous basis)

Referencias

1. Li Guanglei, Chen Yao, Liu Fei, Bi Wenhua, Wang Chenxin, Lu Danfeng, Wen Dan.  (2023)  Portable visual and electrochemical detection of hydrogen peroxide release from living cells based on dual-functional Pt-Ni hydrogels.  Microsystems & Nanoengineering,  9  (1): (1-10).  [PMID:38033990] [10.1038/s41378-023-00623-y]

2. Jie Zhao, Shuaiqian Wang, Diandian Jiang, Yan Lu, Yu Chen, Yong Tang, Jie Tang, Zhenju Jiang, Hongbin Lin, Wei Dong.  (2023)  Unravelling the interaction between α-SOH and myofibrillar protein based on spectroscopy and molecular dynamics simulation.  Food Chemistry-X,      [PMID:38144868] [10.1016/j.fochx.2023.100986]

3. Zhe Chen, Yunxia Wang.  (2023)  A label- and enzyme-free fluorescence assay based on thioflavin T–induced G-quadruplexes for the detection of telomerase activity.  JOURNAL OF CHEMICAL RESEARCH,      [PMID:] [10.1177/17475198221139085]

4. Pengfei Dong, Longyi Zhu, Jing Huang, Jujie Ren, Jianping Lei.  (2019)  Electrocatalysis of cerium metal-organic frameworks for ratiometric electrochemical detection of telomerase activity.  BIOSENSORS & BIOELECTRONICS,      [PMID:31108380] [10.1016/j.bios.2019.05.018]

5. Min Zhang, Chong-sheng Peng, Xiao-bo Li.  (2017)  Human intestine and liver microsomal metabolic differences between C19-diester and monoester diterpenoid alkaloids from the roots of Aconitum carmichaelii Debx..  TOXICOLOGY IN VITRO,      [PMID:28919359] [10.1016/j.tiv.2017.09.011]

6. Feifei Gong, Huaxin Yang, Wen Sun, Junzi Cao, Wanhui Liu.  (2015)  Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances.  ELECTROPHORESIS,  37  (4): (623-629).  [PMID:26635325] [10.1002/elps.201500328]

Preguntas frecuentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥98% purity, determined by HPLC. Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at room temperature.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 331717-45-4, the molecular formula is C32H60N2O8S, and the molecular weight is 614.88 (anhydrous basis) g/mol. InChIKey SJCUTFKCLFLIFE-JWTJKVBLSA-N.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.