DECTIN 2 Antibody - Anticuerpo primario, specific to CLEC6A, Rabbit IgG

Cat. No.: Ab099525

GRADE & PURITY Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

WB

Host species

Conejo

Reactive species

consejo, Humano, Ratón

Isotype

Rabbit IgG

Conjugation

Unconjugated

Descripción general

Rabbit anti Human DECTIN 2 Antibody, Polyclonal antibody, could be used for WB and so on.
Application：
WB: 1/500-1/1000
Protein Function：
Binds high-mannose carbohydrates in a Ca(2+)-dependent manner. Functional receptor for alpha-mannans on C.albicans hypheas. Plays an important role in the host defense against C.albicans infection by inducing TH17 cell differentiation. Recognizes also, in a mannose-dependent manner, allergens from house dust mite and fungi, by promoting cysteinyl leukotriene production. Recognizes soluble elements from the eggs of Shistosoma mansoni altering adaptive immune responses. Transduces signals through an Fc receptor gamma chain /FCER1G and Syk-CARD9-NF-kappa-B-dependent pathway.

Specifications

Product Name

DECTIN 2 Antibody - Anticuerpo primario, specific to CLEC6A, Rabbit IgG

Sinónimos

CLEC4N | CLEC6A | CLECSF10 | dectin-2 | hDECTIN-2 | C type lectin domain family 6 member A antibody | C type lectin superfamily member 10 antibody | C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 10 antibody | C-typ

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Especies hospedadoras

Conejo

Especificidad

CLEC6A

Inmunógeno

A KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Dectin 2.

Control positivo

WB: Mouse heart, Rat heart, Mouse liver lysates.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Isotipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificación

Immunogen affinity purified

Concentración

1.0 mg/mL

Product Line

Productos biológicos

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: DECTIN 2 Antibody (Ab099525)

DECTIN 2 Antibody (Ab099525) - Western Blot
All lanes: DECTIN 2 Antibody (Ab099525) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 24 kDa
Exposure time: 60.1 s

Aplicación

Aplicación

Dilution info

WB

1/500-1/1000

Objetivos asociados (humanos)

CLEC6A Tbio C-type lectin domain family 6 member A (0 Activities)

Discover Target: CLEC6A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Application Protocols

Item-specific (from Product Data)

• Tested/validated applications, recommended dilutions, and positive controls: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general guidance (to be optimized by the user)

• Western blot (WB): Start at 0.2–1 µg/mL primary; validate on lysates known to express DECTIN-2 (e.g., dendritic cell-derived samples). Use appropriate reducing/non-reducing conditions based on target form.
• Flow cytometry (FC): Titrate from 0.05–1 µg per 10^6 cells. Include Fc-blocking steps for myeloid populations to reduce nonspecific FcγR binding.
• IHC/ICC: Begin at 1–10 µg/mL; optimize antigen retrieval for membrane receptors. Validate specificity using isotype controls and knockdown/knockout where available.
• ELISA: For capture/detection, determine pairing compatibility empirically; carrier-free format is suitable for direct conjugation to HRP/biotin.
• Immunoprecipitation (IP): 1–5 µg per mg lysate protein as a starting point; adjust based on affinity and expression.

Note: These are general starting recommendations only. Establish optimal conditions empirically and consult the lot-specific CoA for any validated methods.

Biological Roles

Item-specific (from Product Data)

• Target name provided by product: DECTIN 2 (also known as CLEC6A). No further target details are specified for this item.

Literature/general background on DECTIN-2 (CLEC6A)

• Classification: A type II transmembrane C-type lectin receptor (CLR) expressed primarily on myeloid lineage cells (e.g., dendritic cells, macrophages).
• Ligand recognition: Binds high-mannose glycans and α-mannans commonly present on fungal cell walls. Recognition engages innate immune signaling against such pathogen-associated molecular patterns.
• Signaling: Associates with the FcRγ adaptor bearing an ITAM motif, recruiting SYK kinase, and activating CARD9–BCL10–MALT1 signaling that culminates in NF-κB activation. This pathway promotes production of pro-inflammatory mediators and supports Th17-skewing cytokine responses (literature).
• Functional context: Involved in antigen uptake, cytokine induction, and shaping of adaptive responses via dendritic cell activation. Cross-talk with other CLRs (e.g., MINCLE, Dectin-1) and TLR pathways modulates the quality and magnitude of responses (literature).
• Research utility: Antibodies to DECTIN-2 are used to map expression, track maturation states of myeloid cells, study CLR signaling assemblies, and assess ligand-binding or receptor internalization. No item-specific performance claims are made here; consult CoA for tested applications and species reactivity.

Buffer Applications

Item-specific (from Product Data)

• Specific buffer composition and recommended diluents: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general guidance for antibody buffers

• Working buffers: Phosphate-buffered saline (PBS, pH 7.2–7.4) or Tris-buffered saline (TBS) are common for dilution and storage. For ELISA/IHC, add blocking agents (e.g., 1–5% BSA or non-fat milk) as assay-appropriate; avoid carriers if they interfere with detection or conjugation.
• Stabilizers: For carrier-free antibodies, consider adding 0.05–0.1% BSA and/or 2–5% trehalose post-reconstitution to reduce adsorption and freeze–thaw damage, provided these do not conflict with your assay.
• Detergents: 0.01–0.05% Tween-20 in wash/dilution buffers can reduce nonspecific binding in ELISA/WB; assess impact on antigen–antibody interactions.
• pH/ionic strength: Maintain near-neutral pH (6.8–7.6). Avoid extreme pH or high salt unless required by a protocol (e.g., stringent washes).
• Sterility: Use sterile, low-endotoxin buffers for cell-based assays.

Note: No item-specific buffer recipes or pH ranges are supplied for this product listing.

Green Alternatives

Antibodies themselves are not solvents or process reagents; “green alternatives” considerations focus on sustainable handling and reducing waste.

Literature/general guidance

• Formulation choices: Carrier-free products avoid stabilizers like BSA and glycerol at shipment, potentially reducing cold-chain mass and downstream protein waste when custom formulations are required. Post-reconstitution, choose minimal yet effective stabilizers.
• Storage optimization: Aliquot to avoid repeated freeze–thaw, which reduces discarded, degraded material. Consider -20°C with cryoprotectants (e.g., trehalose) instead of -80°C if validated for stability, lowering energy use.
• Packaging and consumables: Use reusable cold packs and minimize single-use plastics where practical; select low-bind, recyclable tubes when compatible.
• Process alternatives: For conjugation workflows, prefer aqueous, room-temperature chemistries and avoid excess organic solvents. Employ micro-scale optimization to cut reagent consumption.
• Waste: Segregate non-hazardous aqueous protein waste from solvent waste; neutralize and drain per local regulations to reduce incineration volume.

Item-specific

• No additional item-specific green attributes are provided. For lot-specific information (e.g., concentration to plan minimal-use aliquots), refer to the CoA/Spec Sheet.

Pharmaceutical Uses

Item-specific (from Product Data)

• Regulatory/compendial status and intended use: Not specified. Research use only.

Literature/general context

• Research antibodies like this product are used in non-clinical workflows, including target validation, biomarker discovery, analytical testing, and process development assays. They may serve in immunoassays for in-process control or release testing in biomanufacturing research settings, provided they meet internal quality requirements.
• Formulation/excipient considerations: Carrier-free format is advantageous when users need full control over excipients for method compatibility (e.g., avoiding BSA in mass spectrometry-based immunocapture).
• No therapeutic, diagnostic, or clinical use is implied or supported by this listing. For GMP or diagnostic applications, procure appropriately qualified materials and documentation.

Physical Properties

Item-specific (from Product Data)

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight, molecular formula: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general information

• Typical antibody molecular mass: ~150 kDa for full-length IgG; ~50 kDa (heavy chain) and ~25 kDa (light chain) under reducing SDS-PAGE (literature).
• Physical state: Antibodies are typically supplied lyophilized or in aqueous buffer. Carrier-free products commonly arrive lyophilized or in simple saline/PBS without protein stabilizers (literature).
• Solubility: Readily soluble in aqueous buffers (e.g., PBS, Tris-buffered saline). Insoluble in most organic solvents (literature).
• pI/logP/BP/MP/refractive index: Not applicable to macromolecular proteins in the same sense as small molecules; values are sequence- and glycosylation-dependent (literature). No item-specific values provided.
• Stability considerations (general): Stability is influenced by temperature, pH (commonly stable near neutral pH), ionic strength, and freeze–thaw history. Addition of carrier proteins (e.g., 0.1% BSA) or sugars (e.g., 2–5% trehalose) can improve stability when compatible with downstream assays (literature). Always validate compatibility for your application.

Quality and Grades

Item-specific (from Product Data)

• Grade/Purity: Carrier Free, ExactAb™, Validated.

What these designations typically imply (literature/general)

• Carrier Free: The antibody is supplied without carrier proteins (e.g., BSA) or often without glycerol. This reduces potential assay background, avoids interference in conjugation chemistries (e.g., NHS-ester labeling, maleimide coupling), and facilitates accurate protein quantification. It also places greater responsibility on the user to stabilize appropriately after reconstitution (see Storage & Reconstitution).
• ExactAb™: A proprietary branding indicating a defined manufacturing/validation process. Without item-specific criteria disclosed here, interpret as a quality/system brand rather than a technical specification. For exact acceptance criteria (purity, endotoxin, residuals), refer to the CoA/Spec Sheet.
• Validated: Indicates the product has undergone application-appropriate testing. However, specific validated applications, species reactivity, and performance metrics are not provided here. See Application Protocols or request validation data/CoA.

Recommendations

• If planning chemical conjugation (biotin, fluorophores, enzymes), carrier-free format is preferred; verify buffer composition and remove amines/thiols that could compete in coupling.
• For regulated workflows, request lot-specific CoA, including concentration, purity assessment method (e.g., SEC-HPLC, SDS-PAGE), endotoxin (for cell-based work), and residual preservatives (if any).

Reaction and Applications

This section is not typically applicable to antibodies as they are not used as reagents in organic transformations. They function as affinity reagents for detection, capture, and quantification of specific antigens.

Literature/general laboratory applications

• Immunoassays: Western blot (WB), ELISA, immunohistochemistry (IHC), immunocytochemistry (ICC), flow cytometry (FC), immunoprecipitation (IP), and proximity-based assays. Specific validated applications for this product are not provided here; see Application Protocols or request validation data.
• Capture/affinity: Antibody immobilization on solid supports (magnetic beads, agarose, biosensors) for antigen enrichment; carrier-free format is advantageous for clean coupling chemistry.
• Conjugation: Generation of labeled antibodies (HRP, AP, fluorophores, biotin) for detection. Carrier-free buffers with low amine content facilitate NHS-ester labeling; maleimide chemistry targets reduced sulfhydryls.
• Functional studies: Neutralization or blocking assays may be feasible depending on epitope and affinity; no item-specific neutralization claims are made.

Practical tips (general)

• Validate working concentration and blocking strategy for each assay matrix.
• Include isotype and secondary-only controls where applicable.
• Use low-binding plastics and pre-blocked surfaces to minimize loss for carrier-free preparations.

Reaction Conditions

Not typically applicable to antibodies with respect to chemical reaction conditions.

Literature/general guidance for handling conditions

• Temperature: Keep on ice during bench work; store at -20°C per item-specific guidance. Avoid elevated temperatures that promote denaturation.
• pH: Near-neutral pH (6.8–7.6) maintains stability for most IgGs; verify compatibility with your application.
• Time: Limit bench exposure; minimize total handling time to reduce adsorption and aggregation.
• Light: Protect from strong light if conjugated to fluorophores/enzymes (no item-specific conjugation is stated here).
• Conjugation reactions (general): For NHS-ester labeling, conduct at pH 7.5–8.5 in amine-free buffers; for maleimide-thiol coupling, use pH 6.5–7.5. Reaction times typically range 30–120 minutes at room temperature, followed by desalting or dialysis. These are general guidelines, not item-specific conditions.

Safety and Handling

Item-specific (from Product Data)

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS.
• Storage conditions: Store at -20°C. Avoid repeated freezing and thawing. Shipped in an ice chest with ice pads.

General laboratory safety (literature/guidance; defer to SDS and institutional policies)

• PPE: Wear lab coat, disposable nitrile gloves, and safety glasses when handling protein reagents and buffers.
• Biosafety: Although research-grade antibodies are non-infectious, handle as biological materials using good microbiological practices; avoid aerosol generation and ingestion.
• Incompatibilities: Avoid strong oxidants, extreme pH, and organic solvents that denature proteins. Surfactants and preservatives may affect certain assays; evaluate compatibility before use.
• Handling: Minimize freeze–thaw cycles; aliquot upon first thaw/reconstitution. Keep on ice during use to reduce proteolysis/denaturation.
• First aid (overview): In case of skin/eye contact, rinse with water. If inhaled, move to fresh air. If ingested, rinse mouth and seek medical attention per SDS.
• Waste: Dispose according to local regulations for aqueous, non-hazardous laboratory biologicals unless your formulation includes hazardous additives; consult SDS.

Solvent Selection

Item-specific (from Product Data)

• Formulation details/solvent: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general guidance for antibodies

• Primary medium: Aqueous physiological buffers (e.g., PBS pH ~7.2–7.4, 10–50 mM; Tris-buffered saline) are preferred for solubilization and storage.
• Miscibility: Antibodies are not compatible with most organic solvents; even small percentages of acetonitrile, methanol, or DMSO can denature proteins. If co-solvent is unavoidable for assay components, keep organic content minimal and validate stability.
• Additives: For carrier-free antibodies, stabilizers like 0.05–0.1% carrier protein (BSA) or 2–5% disaccharides (trehalose, sucrose) may be added post-reconstitution if compatible with downstream use. Low levels (0.01–0.05%) of non-ionic surfactants (Tween-20) can reduce adsorption losses in some assays; assess interference.
• Comparison to alternatives:
  • Protein-friendly: PBS, HEPES, MOPS (near-neutral pH) maintain native fold.
  • Not recommended: Strong acids/bases, chaotropes, high salt (>1 M) unless specifically required for purification or denaturation workflows.
• Practical tip: For conjugation, avoid primary amine buffers (e.g., Tris) during NHS-ester reactions; use PBS or carbonate/bicarbonate as appropriate.

Storage and Reconstitution

Item-specific (from Product Data)

• Storage: Store at -20°C. Avoid repeated freezing and thawing.
• Shipping: Ice chest with ice pads.
• Reconstitution instructions and buffer composition: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general guidance

• Upon receipt: If lyophilized, briefly centrifuge to collect material before opening. Reconstitute with sterile, neutral pH buffer (e.g., PBS) to a convenient stock concentration. If supplied in solution and carrier-free, consider immediate aliquoting.
• Aliquoting: Prepare single-use aliquots in low-bind tubes to prevent adsorption and freeze–thaw damage.
• Stabilization (optional, if assay-compatible): Add 2–5% trehalose and/or 0.05–0.1% carrier protein (e.g., BSA) after reconstitution to improve freeze–thaw stability. Avoid adding carriers if planning chemical conjugation or if they interfere with assays.
• Storage temperature: Maintain at -20°C as specified. For short-term (days to 1–2 weeks), 2–8°C may be acceptable if preservative is present and validated; no preservative is specified for this item.
• Freeze–thaw: Avoid repeated cycles. Thaw on ice and mix gently by inversion; do not vortex vigorously.
• Documentation: Consult the CoA/Spec Sheet for lot-specific concentration, buffer, preservative (if any), and recommended reconstitution volume.

Structure and Identity

Item-specific (from Product Data)

• Product: DECTIN 2 Antibody (SKU: Ab099525)
• Grade/Purity: Carrier Free, ExactAb™, Validated
• Structural identifiers (SMILES, InChIKey, CAS, CID, molecular formula): Not specified for this item; refer to CoA/Spec Sheet.

Literature/general information

• Antibody type: Immunoglobulin protein reagent targeting DECTIN-2 (also known as CLEC6A; C-type lectin domain family 6 member A). Specific clone, host species, and isotype are not provided for this item.
• Structural features (general for IgG): Y-shaped heterotetramer (~150 kDa) comprising two heavy and two light chains linked by disulfide bonds; variable regions (VH/VL) confer antigen binding; constant regions (CH/CL) define isotype/effector functions. Carrier-free indicates absence of protein stabilizers such as BSA and typically no glycerol (see Quality & Grades).
• Target (general): DECTIN-2 is a type II transmembrane C-type lectin receptor with a carbohydrate recognition domain (CRD) that binds high-mannose structures; not part of the antibody’s structure but relevant to target identity.
• 2D description in words (general): As a protein macromolecule, the antibody lacks a simple small-molecule 2D depiction; it features beta-sandwich immunoglobulin folds in Fab domains forming the antigen-binding paratope, and an Fc domain with glycosylation sites.

Synthetic Utility

Not typically applicable. Antibodies are biological affinity reagents and are not used as building blocks or reagents in chemical synthesis.

Literature/general notes

• Chemical conjugation: While not synthetic reagents, antibodies can be chemically modified (biotinylation, fluorophore/enzyme labeling, PEGylation) via lysine (NHS-esters), cysteine (maleimides after reduction), or glycan-directed methods (periodate oxidation followed by oxime formation). Carrier-free preparations are advantageous for predictable conjugation stoichiometry.
• Affinity matrices: Antibodies can be immobilized on activated supports (e.g., NHS-activated Sepharose) to create highly specific affinity resins for antigen capture from complex mixtures.

Target Specificity

Item-specific (from Product Data)

• Antigen/epitope: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity: Not specified for this item; refer to CoA/Spec Sheet.
• Clone, host species, isotype: Not specified for this item; refer to CoA/Spec Sheet.

Literature/general context

• Target: DECTIN-2 (CLEC6A), a C-type lectin receptor recognizing high-mannose/α-mannan ligands. Common experimental epitopes include extracellular domain peptides or recombinant ectodomains; actual immunogen/epitope for this product is not provided.