Dextranase from Chaetomium erraticum, CAS No.9025-70-1

CAS: 9025-70-1 Cat. No.: D298997 EC Number: 232-803-9 PubChem CID: 135273372

GRADE & PURITY EnzymoPure™, ≥100 KDU-A/g

Bioactivity

≥100 KDU-A/g

Overview

Dextranase hydrolyzes the (1,6)-alpha-D-glucosidic linkages in dextran.

This product is a fungal dextranase produced by submerged fermentation of Chaetomium erraticum.

It has many industrial and clinical applications such as food processing, sugar modification, and medicine preparation. Dextranase is widely used to produce alternated sugar beet and isomaltooligosaccharides (IMOs), a prebiotic functional food. The low molecular weight dextran obtained from dextranase serves as a synthetic blood volume expander. Dextranase can be coupled to antibodies for the detection of tumor cells. It prevents the occurrence of dental plaques by depolymerizing microbial dextran deposits in teeth. Hence, it is used in dental care products.

Stable in the pH range of 3-7 and at temperatures up to approx. 70 °C. For most applications, the preferred conditions are pH 5-6 and a temperature of 50-60 °C.

Application

Dextranase from Chaetomium erraticum has been used:

in microcarrier dissolution to produce multicellular 3D spheroids for drug discovery,

in in vitro digestion of extracellular polysaccharide (EPS) from the bacterial isolates,

in a study to investigate the immobilization of dextranase,

to investigate the optimization of process conditions for enzymatic modification of alternan.

Genetic Modification Status

This product is not a genetically modified organism (GMO).

Enzyme preparations are produced by microbial fermentation, with no residual microorganisms in the final product. The microorganisms used for production have not been modified by modern biotechnology.

Storage Conditions

Storage temperature: 2–8℃.

The original packaging must be kept intact during storage. Store in a dry environment and avoid direct sunlight.

Use the product in accordance with the recommended usage and prior to the expiration date of the shelf life to avoid the need for increased dosage.

Safety and Handling Precautions

Enzymes are proteins. Inhalation of dust or aerosols may cause sensitization and trigger allergic reactions in sensitized individuals.

Prolonged contact with some enzymes may cause irritation to the skin, eyes and mucous membranes.

Specifications

Product Name

Dextranase from Chaetomium erraticum, CAS No.9025-70-1

Synonyms

Dextranase Plus L | 1, 6-α-D-Glucan 6-glucanohydrolase | Sustine 470

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, ≥100 KDU-A/g

Bioactivity

≥100 KDU-A/g

CAS

9025-70-1

Enzyme Commission Number

EC 3.2.1.11

Molecule Type

Enzyme

Legal Information

Dextranase Plus L of Novozymes Corp.

Storage and Shipping

Concentration

≥100 KDU-A/g

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Unit definition

One KDU-A is defined as the amount of enzyme required to hydrolyze dextran to produce 1 gram of maltose-equivalent reducing sugars per hour under the Novozymes standard assay conditions.

References

1. Jiaqi Qiu, Siyuan Xiang, Miyao Sun, Mingqian Tan.  (2023)  Preparation of Polysaccharide–Protein Hydrogels with an Ultrafast Self-Healing Property as a Superior Oral Delivery System of Probiotics.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,      [PMID:37978937] [10.1021/acs.jafc.3c05898]

2. Jun Huang, Gang Yang, Kewei Chen, Muying Du, Zsolt Zalán, Ferenc Hegyi, Jianquan Kan.  (2024)  Anti-fungal effects of lactic acid bacteria from pickles on the growth and sterigmatocystin production of Aspergillus versicolor.  INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,      [PMID:38955023] [10.1016/j.ijfoodmicro.2024.110809]