EBP-IN-1 - Moligand™, 10 mM in DMSO

Cat. No.: E1498503

CALIDAD & PUREZA Moligand™, 10 mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Descripción general

EBP-IN-1 (compound 11) is an inhibitor of emopamil-binding protein (EBP), a sterol isomerase in the cholesterol biosynthetic pathway. EBP-IN-1 has a long half-life in rodents and has good metabolic turnover and brain penetration properties. EBP-IN-1 enhances oligodendrocyte formation in human cortical organoids.

Especificaciones

Especificaciones y pureza

Moligand™, 10 mM in DMSO

Condiciones de almacenamiento de almacenamiento

Store at -80°C

Enviado en

Dry ice packs + Cold packs

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

Moligand™

Application Protocols

No internally validated, item‑specific protocols are provided for this SKU.

General guidance for small‑molecule use in assays (not item‑specific):

• Reconstitution: Prepare a concentrated DMSO stock under dry conditions; record exact concentration by weight or quantitative NMR if high precision is required.
• Dosing: Dilute into assay buffer or media with vigorous mixing to the desired concentration, ensuring the final cosolvent fraction is within your system’s tolerance. Include vehicle controls.
• Plate handling: Use low‑binding plastics or glass vials for storage and transfer to minimize adsorption losses. Pre‑wet tips with solution before dispensing small volumes.
• Stability: Minimize freeze–thaw cycles by aliquoting single‑use volumes. Protect from light if the compound contains chromophores prone to photolysis (unknown for this item).

For detailed, validated procedures, consult your laboratory SOPs. Any method development should be accompanied by analytical verification (LC–MS/HPLC) of concentration and integrity.

Biological Roles

Item‑specific biological target(s), pathways, and mechanism of action are not provided for this SKU. The name “EBP‑IN‑1” suggests a small‑molecule inhibitor in a discovery context, but without supplied identifiers no definitive biological role can be asserted for this product.

General context for small‑molecule inhibitors (literature, not item‑specific):

• Small molecules can modulate protein function via orthosteric binding to active sites or allosteric binding that alters conformation. Inhibition modalities include competitive, non‑competitive, uncompetitive, and covalent mechanisms.
• In chemical biology, such ligands are used to interrogate signal transduction, enzyme catalysis, and protein–protein interactions, enabling temporal control compared with genetic perturbations.
• Target engagement methods include CETSA, BRET/NanoBRET, FRAP, and chemoproteomic capture when suitable handles are present.

Assay considerations:

• Establish the free (unbound) concentration at the site of action by considering protein binding and nonspecific adsorption to plastics.
• Verify selectivity using panel screens or orthogonal assays to reduce the risk of off‑target effects or assay interference (e.g., fluorescence quenching, redox activity).

To define the biological role for your lot, consult the CoA/Spec Sheet and any reference documents or publications cited therein.

Buffer Applications

This product is not a buffering agent and is not typically used to set or maintain pH. Accordingly, a buffer selection guide is not directly applicable.

Practical notes for using small molecules in buffered systems (general guidance):

• Choose buffers with low UV absorbance in your detection window (e.g., HEPES or phosphate for UV above 220 nm) and minimal reactivity with the compound’s functional groups.
• Maintain ionic strength and pH appropriate for target stability; confirm that the compound remains in solution across the intended pH and salt ranges.
• Limit cosolvent (e.g., DMSO, ethanol) to the lowest effective level to prevent protein denaturation or cell stress. Include vehicle controls.

If you require a recipe or pH range for a specific buffer system, please refer to standard biochemistry references; none are specified for this item.

Green Alternatives

Because this product functions as a research ligand rather than a bulk solvent or reagent, “green” considerations mainly relate to solvent choices for reconstitution and assay use.

Greener handling strategies (general guidance):

• Prefer water‑based buffers with the minimal required co‑solvent fraction. Validate solubility and activity at ≤0.1–0.5% v/v DMSO where feasible.
• Where DMSO is not required, consider ethanol as a more biodegradable alternative, subject to assay tolerance.
• Reduce waste by preparing high‑concentration stocks and performing micro‑scale assays (96/384/1536‑well formats).
• Use glass vials to minimize plastic adsorption and reduce disposable plastics, when compatible with workflow.

Illustrative comparison (general; not item‑specific):

| Option | Pros | Considerations | |---|---|---| | DMSO | Broad solvency; stable; low vapor pressure | Not benign to aquatic life; limit assay % and waste volume | | Ethanol | Renewable content; readily biodegradable | Higher volatility; lower solvency for some scaffolds | | PEG‑400 (co‑solvent) | Low volatility; low acute toxicity | Viscous; may affect assay readouts | | Aqueous buffers | Benign; minimal VOCs | Solubility limits; potential precipitation |

Note: The optimal “green” choice must not compromise data quality. Conduct stability and solubility checks before changing solvent systems.

Pharmaceutical Uses

No pharmacopeial status, excipient role, or clinical formulation information is provided for this product. This material is supplied strictly for research use only.

Context (general, not item‑specific):

• Discovery‑stage ligands may be formulated for exploratory in vivo studies using vehicles such as 10–20% PEG‑400, 10% Solutol HS‑15, or 0.5% methylcellulose with controlled DMSO content. Such guidance is illustrative only and must not be construed as a recommendation for this item.
• There are no therapeutic claims associated with this product listing. Any preclinical or clinical application requires independent qualification of identity, purity, stability, and safety.

If you intend to develop dosage forms or conduct regulated studies, obtain comprehensive characterization data (CoA, stability profile, residual solvents, polymorph information) and determine suitability through your internal QA/QC procedures.

Physical Properties

Item‑specific physicochemical data have not been provided for this SKU.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point / boiling point / density / refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility profile (water/organic) and logP/logD: Not specified for this item; refer to CoA/Spec Sheet.
• pKa values, UV–Vis characteristics, and extinction coefficients: Not specified for this item; refer to CoA/Spec Sheet.

General handling guidance for discovery‑grade small molecules (literature, not item‑specific):

• Many bioactive screening ligands are reconstituted in DMSO to make 10–50 mM stocks, then diluted into assay buffers to ≤0.1–0.5% v/v DMSO. Verify the tolerance of your biological system.
• If aqueous solubility is limited, co‑solvents such as DMF, ethanol, or PEG‑400 can be used in small percentages, or solutions can be prepared by serial cosolvent addition with gentle warming (≤37 °C) and sonication. Validate for your application.
• For UV detection in HPLC/UPLC, many aromatic small molecules show λmax in the 200–300 nm region; specific spectra must be verified experimentally for this item.

Practical tip:

• When physical constants are not available, run a small solubility screen (water, PBS, DMSO, DMF, MeOH, EtOH) and document concentration at clarity. Retain aliquots at working concentrations to minimize freeze–thaw.

Quality and Grades

• Grade/Purity designation: Moligand™

What Moligand™ typically signifies (program-level description):

• The Moligand™ line encompasses discovery‑grade bioactive small molecules suitable for screening, target validation, and chemical biology. Emphasis is placed on fitness for research assays rather than pharmacopeial compliance. This description is general and not a specification of analytical values for this SKU.

Item‑specific quality metrics:

• Quantitative purity, residual solvent levels, water content, stabilizers, and metal limits are Not specified for this item; refer to CoA/Spec Sheet.

Analytical considerations for users:

• For screening and SAR, users commonly confirm identity/purity by LC–MS and HPLC prior to critical studies. Where sensitive assays are involved (e.g., chemoproteomics), consider orthogonal checks (NMR, HRMS) and endotoxin testing for biological compatibility as needed.

Stabilizers and formulation:

• No stabilizer or salt information is provided here. If your application is sensitive to acid/base or antioxidant additives, verify excipients on the lot CoA before use.

Traceability:

• Record the SKU (E1498503) and lot number in your ELN/LIMS. For regulated workflows, retain the CoA and shipment documentation to evidence cold‑chain and storage compliance (−80 °C).

Reaction and Applications

This product is positioned as a small‑molecule research ligand. No item‑specific reactivity or synthetic role has been provided.

Research application domains (general, not item‑specific):

• Biochemical assays: Enzyme activity or binding assays to quantify inhibitory potency (e.g., IC50, Ki) or mechanism (competitive, non‑competitive). Validate with orthogonal readouts to rule out assay interference.
• Cell‑based studies: Pathway modulation, target engagement (e.g., CETSA, DARTS), and phenotypic screening. Monitor cytotoxicity and solubility limits.
• Chemical biology: With appropriate analogs or tags, can inform SAR, photoaffinity labeling, or pull‑down experiments. Such derivatives are not supplied here unless explicitly stated in product data.

Practical considerations:

• Prepare aliquoted DMSO stocks to minimize freeze–thaw and adsorption losses. For sticky compounds, use low‑binding tubes and add small amounts of non‑ionic surfactant (e.g., 0.01% Tween‑80) only if compatible with your assay.
• Include vehicle controls and, if available, a qualified positive control compound.

Not provided for this item:

• Reaction roles (e.g., reagent in named organic reactions), catalytic uses, or material transformation data are not specified. If you intend to employ this compound synthetically, confirm functional groups and stability from the CoA and perform preliminary tests.

Reaction Conditions

There are no specified reaction conditions for this product as a reagent in chemical synthesis.

If derivatization is planned (general guidance; not item‑specific):

• Solvents: DMF, DCM, MeCN, dioxane, THF are common; ensure compatibility with the compound’s stability profile.
• Temperatures: 0–80 °C depending on reaction class; avoid elevated temperatures until thermal stability is known.
• Catalysts/reagents: For amide formation, HATU/EDC/DIC with base (DIPEA) in DMF; for cross‑coupling, Pd(0/II) systems with appropriate ligands in dioxane/water or toluene. These are general examples only.
• Workup/purification: Use reverse‑phase or normal‑phase chromatography as dictated by polarity; monitor by LC–MS.

For biological assays (preparation conditions; general):

• Typical stock solutions are prepared in dry DMSO at 10–50 mM, then diluted into assay buffer to the required final concentration, keeping DMSO ≤0.1–0.5% v/v as feasible.

All conditions must be validated experimentally for your specific lot; no claims are made for this item’s synthetic reactivity.

Safety and Handling

Hazard classification and GHS details specific to this item are not provided here.

• Signal word / H‑statements / pictograms / GHS classification: Not specified for this item; refer to SDS.
• Research use: For research use only. Not for human or veterinary use.

General laboratory safety (applicable best practices):

• Personal protective equipment: lab coat, safety glasses, nitrile gloves. Handle solutions in a fume hood to avoid inhalation of aerosols or solvent vapors.
• Storage conditions: This product is indicated to Store at −80 °C. Protect from moisture and light unless otherwise stated on the CoA. Avoid temperature cycling; maintain cold chain upon receipt.
• Incompatibilities: Absent specific data, avoid strong oxidizers, strong acids/bases, and reactive metals. Use only compatible, dry, inert containers for long‑term storage.
• Peroxide formation: Not assessed; if dissolved in peroxide‑forming solvents (e.g., ethers), monitor solvent quality per institutional policy.

First‑aid overview (consult SDS for authoritative guidance):

• Skin/eye contact: Rinse with water for ≥15 minutes; remove contaminated clothing. Seek medical attention if irritation persists.
• Inhalation: Move to fresh air; seek medical attention if symptoms occur.
• Ingestion: Rinse mouth; do not induce vomiting; seek medical attention.

Waste disposal:

• Dispose in accordance with local regulations. Treat solutions as organic chemical waste unless your SDS/CoA provides alternative instructions.

Solvent Selection

Item‑specific solubility has not been provided for this product. The following guidance reflects typical practice for small‑molecule screening ligands.

Primary reconstitution choices:

• DMSO: Default for many drug‑like molecules; supports concentrated stocks (often 10–50 mM). Low volatility and good compatibility with most biochemical and cell‑based assays at ≤0.1–0.5% v/v.
• DMF or NMP: Useful for very poorly soluble compounds; assess cytotoxicity at low carryover levels.
• Ethanol or Methanol: Alternative co‑solvents with faster evaporation; consider volatility and assay tolerance.

Aqueous handling:

• After preparing a DMSO stock, dilute into buffered saline (e.g., PBS, HEPES, Tris) while vortexing to minimize precipitation. Filter (0.22 µm) only if recovery has been validated.

Selection considerations:

• Target assay type (biochemical vs. cellular), allowed cosolvent ceiling, and adsorption losses to plastics determine the optimal solvent system.

Comparison (general; not item‑specific):

• DMSO: broad solvency; low vapor pressure; potential to modulate membrane properties at >0.5%.
• Ethanol: moderate solvency; higher volatility; generally tolerated up to ~0.5–1% in many cells.
• DMF/NMP: strong solvency; use sparingly due to cytotoxicity.

Action item: Perform a mini solubility/compatibility screen across candidate solvents at your intended assay temperature and composition.

Storage and Reconstitution

• Storage conditions (as provided): Store at −80 °C.
• Shipping: Dry ice packs + Cold packs to preserve cold chain.

Additional best practices (general; not item‑specific):

• Upon receipt, inspect the package integrity and transfer the item to −80 °C promptly. Allow vials to equilibrate to room temperature in a desiccator before opening to avoid moisture condensation.
• If the product is supplied as a solid or film, prepare single‑use aliquots of stock solution (commonly in dry DMSO) to avoid repeated freeze–thaw. Record exact concentrations.
• Protect from light and moisture unless the CoA indicates insensitivity. Use inert gas backfill (argon or nitrogen) for long‑term storage of sensitive materials.
• Working solution stability, aqueous solubility, and precipitation limits are Not specified for this item; refer to CoA/Spec Sheet. Establish your own stability window by LC–MS/HPLC at intended storage temperatures.

Reconstitution example (general, not item‑specific):

• Add dry DMSO to achieve a convenient stock concentration (e.g., 10–50 mM). Vortex and, if needed, sonicate gently. Filter only if recovery is validated. Store aliquots at −20 to −80 °C in tightly sealed vials.

Always defer to the lot‑specific CoA/SDS for authoritative instructions.

Structure and Identity

This product is a small‑molecule research ligand offered under Aladdin’s Moligand™ line. Item‑specific structural identifiers have not been provided for this SKU.

• Product name: EBP-IN-1 (catalog descriptor)
• SKU: E1498503
• CAS / InChIKey / SMILES / IUPAC: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula / molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General structural context (literature, not item-specific):

• Compounds labeled “IN-1” in discovery workflows typically denote a first‑in‑series small‑molecule inhibitor or probe; these are often drug‑like scaffolds (heteroaromatics, amide/urea linkages, or biaryl systems) designed to engage a protein target via H‑bonding and hydrophobic contacts. This is general information and not a specification for this item.

2D structure description (general guidance):

• In the absence of a provided structure, no ring systems, stereochemistry, or functional groups can be asserted for this specific product. If your work requires exact identifiers, please request the current CoA/Specification Sheet for E1498503 or consult the lot-specific analytical documentation supplied with your shipment.

Notes for identity confirmation:

• For internal QC, common techniques include HRMS, 1H/13C NMR, and HPLC/UPLC purity profiling. Specific analytical results for this item are not listed here and should be sourced from the lot CoA.

Synthetic Utility

No item‑specific functional groups or reactivity are disclosed; therefore, defined synthetic roles cannot be assigned for this product.

General considerations (literature, not item‑specific):

• Drug‑like small molecules commonly feature heteroaromatic cores, amide/urea linkages, and privileged motifs that can be diversified for structure–activity relationships (SAR). If your program involves derivatization of this scaffold, typical transformations include cross‑couplings (Suzuki, Buchwald–Hartwig), amide couplings (HATU/EDC), and heteroatom alkylations/acylations.
• Late‑stage diversification can be approached via C–H functionalization or photoredox strategies, contingent on the scaffold.

User actions:

• Confirm the exact structure from the CoA/Spec Sheet before planning synthetic elaboration.
• Assess chemical stability to acid/base, oxidants, light, and heat to inform protection strategies and purification methods.

Absent explicit structural information, treat this product primarily as a biological probe rather than a synthetic building block.

Target Specificity

Target annotations, binding partners, and selectivity profiles are not specified for this item. No antigen/epitope or target class information is provided in the product data for E1498503.

• Tested species, biochemical target, isoform selectivity, and off‑target screening results: Not specified for this item; refer to CoA/Spec Sheet.

If target information is required, please contact us with your lot number to obtain any available characterization data or references.

Preguntas frecuentes

What is Moligand??

Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.