GRADE & PURITYMoligand™?Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools.≥95%
Moligand™,≥95% Moligand™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
Ecopladib inhibits cPLA2α in the PAPE liposome assay at 73% at a concentration of 37 nM, while it inhibits sPLA2 at 16% at 1 μM. Ecopladib inhibits the production of prostaglandins (PGF2α) and leukotrienes (LTB4 and LTC4/D4/E4) with comparable IC 50 s of 20−30 nM. Ecopladib is inactive against COX-1 and COX-2 at 20 μM, which is nearly 100 times the IC 50 in the MC-9 cells. Ecopladib inhibit 12- and 15-HETE, which are derived from arachidonic acid via the 12- and 15-lipoxygenase pathways and the IC 50 s are ∼0.3 μM. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Ecopladib is a sub-micromolar inhibitor of cytosolic phospholipase A2α (cPLA2α) , with IC 50 s of 0.15 μM and 0.11 μM in the GLU micelle and rat whole blood assays, respectively.
Condiciones de almacenamiento de almacenamiento
Store at -20°C
Enviado en
Ice chest + Ice pads
Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.
This compound belongs to the class of organic compounds known as diphenylmethanes. These are compounds containing a diphenylmethane moiety, which consists of a methane wherein two hydrogen atoms are replaced by two phenyl groups.
External Descriptors
Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Propiedades químicas y físicas
Peso molecular
748.100 g/mol
XLogP3
9.500
Hydrogen Bond Donor Count
2
Hydrogen Bond Acceptor Count
6
Rotatable Bond Count
14
Exact Mass
746.118 Da
Monoisotopic Mass
746.118 Da
Topological Polar Surface Area
106.000 Ų
Heavy Atom Count
50
Formal Charge
0
Complexity
1150.000
Isotope Atom Count
0
Defined Atom Stereocenter Count
0
Undefined Atom Stereocenter Count
0
Defined Bond Stereocenter Count
0
Undefined Bond Stereocenter Count
0
The total count of all stereochemical bonds
0
Covalently-Bonded Unit Count
1
Calculadoras de soluciones
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Reseñas
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Application Protocols
No item-specific, validated application protocols are provided for this product. The following generic guidance applies to small-molecule test articles:
Stock solution: Prepare in anhydrous DMSO (e.g., 10–50 mM). Record exact concentration gravimetrically or by qNMR/UV if the extinction coefficient is known.
Plate handling: Dispense using acoustic or low-retention tips. Include vehicle controls matching the highest DMSO content used.
Dose–response: Typical 8–12 point, 3-fold serial dilutions covering at least 2 logs around the expected activity range. Run in technical triplicates and at least two biological replicates.
Counterscreens: Incorporate aggregation, redox, and fluorescence interference controls appropriate to your assay modality.
Data integrity: Confirm identity/purity of working stock by LC–MS before extended studies.
For detailed, application-specific SOPs, develop and qualify methods in-house or contact our technical support with your assay requirements.
Biological Roles
Item-specific biological targets, mechanisms, and pathways are not provided in the Product Data and should not be inferred without validated references.
General context for small-molecule research tools (non-item-specific)
Small molecules like this entry are often used as chemical probes or ligand candidates to interrogate biological systems in vitro. Their roles may include modulation of enzyme activity, receptor binding, pathway perturbation, or serving as positive/negative controls in assay development.
Prior to biological testing, confirm compound identity and purity (CoA) and assess stability under assay conditions (light, pH, redox environment). Evaluate nonspecific effects (detergent-sensitive aggregation, redox cycling, PAINS liabilities) using appropriate counterscreens.
For cell-based work, determine solvent tolerance, perform dose–response profiling with adequate replicates, and include vehicle controls and orthogonal assays to substantiate biological findings.
Important
No medical, diagnostic, or therapeutic claims are made for this product. It is supplied strictly for research use only. Users are responsible for establishing biological roles and targets via their own validation pipelines.
Buffer Applications
This item is a discrete small molecule and not a conventional buffering agent. Therefore, defined buffer systems (e.g., phosphate, HEPES, Tris) are not directly applicable to the compound itself.
Practical notes for assays
Prepare concentrated DMSO stocks and dilute into your chosen assay buffer (PBS, HEPES, Tris, etc.), keeping final DMSO within assay-tolerated limits (commonly 0.1–1% v/v).
Check for precipitation upon dilution and adjust ionic strength, pH, or include low levels of solubilizing excipients (e.g., 0.01–0.05% nonionic surfactant) if compatible with your assay.
Validate compound stability in the chosen buffer over the assay timeframe (e.g., 0–24 h) by LC–MS or HPLC.
Green Alternatives
While the compound itself is fixed, sustainability choices can be made in its handling and analytics.
Greener solvent choices (general guidance)
Prefer water-buffer systems for assays where feasible, with minimal DMSO cosolvent (≤0.1–0.5% v/v) to reduce solvent burden.
For processing and cleaning, consider ethanol or isopropanol in place of chlorinated solvents when compatible with the compound’s stability and solubility.
For extractions/workups, ethyl acetate can replace dichloromethane in many protocols; evaluate recovery and stability.
Comparison (general, non-item-specific)
DMSO vs DMF: DMSO offers strong solvency with a more favorable environmental and toxicological profile than DMF for routine stock preparation.
MeCN vs Acetone/Ethanol: Acetonitrile has excellent chromatographic properties but higher waste impact; ethanol/acetone may substitute for some cleaning and sample prep steps if the analyte is stable.
Operational practices
Use micro-aliquoting to minimize freeze–thaw and reduce wastage.
Consolidate LC–MS runs and adopt UPLC to cut solvent consumption.
Segregate waste streams (aqueous/organic/halogenated) to facilitate compliant disposal and potential solvent recovery.
Note
Always verify that alternative solvents do not compromise compound integrity or assay performance. Conduct small pilot tests before broad implementation.
Pharmaceutical Uses
No clinical or therapeutic uses are claimed. However, in a pharmaceutical R&D context, such small molecules may serve the following research and development roles:
Analytical reference standard: Use to develop and qualify LC–UV/LC–MS methods, assess recovery, and establish stability-indicating conditions in preclinical matrices, after confirming identity/purity from the CoA.
Assay control or tool compound: Employed as a positive/negative control in biochemical or cellular assays during target validation and lead optimization, pending user-established activity.
Formulation feasibility studies: Evaluate solubilization strategies (cosolvents, pH adjustment, cyclodextrins, lipid-based systems) for discovery-stage in vitro dosing. Document excipient compatibility and precipitation limits.
Metabolism/stability investigations: Study microsomal/S9 stability, protein binding, and photostability to inform discovery decisions. Ensure appropriate bioanalytical coverage using validated internal standards.
Regulatory and compendial status
No pharmacopeial monograph is claimed or implied for this product. Any filing/registration uses require independent qualification and documentation by the user.
Reminder
Supplied strictly for research use only; not for human or veterinary use, diagnostic procedures, or clinical applications.
Physical Properties
Item-specific physicochemical constants are not provided in this catalog entry.
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Melting point (MP): Not specified for this item; refer to CoA/Spec Sheet.
Boiling point (BP): Not applicable/Not typically reported for discrete bioactive solids; refer to CoA.
Density: Not specified for this item; refer to CoA/Spec Sheet.
Solubility: Not specified for this item; refer to CoA/Spec Sheet. Practical guidance provided below (general/literature-based).
LogP / pKa / refractive index: Not specified for this item; refer to CoA/Spec Sheet.
General handling guidance (literature/practice, not item-specific)
Many screening-grade small molecules are supplied as crystalline or amorphous solids with good solubility in DMSO and variable solubility in alcohols, acetonitrile, or aqueous buffers depending on ionization state.
Where structure is available, estimate solubility and ionization using standard cheminformatics (e.g., predicted cLogP, pKa) prior to assay setup.
For aqueous work, prepare concentrated DMSO stocks (e.g., 10–50 mM) and dilute into buffer, keeping final DMSO ≤0.1–1% v/v as assay-compatible.
Important
Do not treat the above as specifications. For batch-relevant values (water content, residual solvents, particle size, UV cut-off, etc.), consult the CoA. If your workflow requires defined polymorph or salt form, confirm with our technical support before use.
Quality and Grades
Grade/Purity: Moligand™ (as provided)
What Moligand™ signifies
Moligand™ designates compounds curated for small-molecule research, screening, and ligand discovery workflows. Typical expectations include: identity verified by spectroscopic methods, screening-suitable purity, and packaging optimized for aliquoting and cold-chain logistics. Exact analytical criteria can vary by item.
Item-specific details
Purity: Not specified for this item; refer to CoA/Spec Sheet.
Stabilizers/Additives: Not specified for this item; refer to CoA/Spec Sheet.
Salt/solvate state and polymorph: Not specified for this item; refer to CoA/Spec Sheet.
Implications for use
For HTS/SAR: verify purity and form (free base vs. salt) to calculate accurate molar concentrations in DMSO stocks.
For analytical work (e.g., LC–MS reference): request the latest batch CoA to confirm purity basis (HPLC/UPLC area %, qNMR, or other) and residual solvent levels.
For biophysical assays (SPR/ITC/DSF): ensure buffer compatibility and confirm absence of UV-active impurities if measuring at 220–280 nm.
Traceability and documentation
Record SKU (E651115), lot number, and any on-receipt QC performed. Retain CoA/SDS with experimental records to meet publication and audit requirements.
Reaction and Applications
This product is positioned as a research-grade small molecule for discovery workflows rather than a synthetic reagent. Accordingly, its primary “applications” are in screening, target validation, and method development, not as a substrate or catalyst in preparative chemistry.
Representative research uses (general guidance)
Biochemical/biophysical assays: Evaluate as a test article in enzyme or receptor assays, thermal shift (DSF), isothermal titration calorimetry (ITC), or surface plasmon resonance (SPR) once the user-defined target and assay conditions are established.
Cell-based assays: Prepare DMSO stocks and perform concentration–response studies with appropriate vehicle controls and cytotoxicity counterscreens. Validate compound integrity by LC–MS before extended campaigns.
Analytical development: Use as a reference material for LC–MS method setup, recovery studies, or stability-indicating methods in relevant matrices (buffers, microsomes), after confirming identity by CoA.
Not a general-purpose reagent
Named reactions, catalytic roles, or functional group transformations are typically not applicable to a finished, bioactive small molecule. If you plan derivatization (e.g., biotinylation, photoaffinity tagging), obtain or infer the structure from CoA/database and consider protecting groups and compatibility prior to synthesis.
Reaction Conditions
Not typically applicable. This product is not offered as a reagent or catalyst for synthetic transformations, and no item-specific reaction conditions are provided.
General experimental conditions relevant to handling as a test article (non-synthetic)
Stock preparation: Dissolve in anhydrous DMSO at an appropriate concentration (e.g., 10–50 mM), vortex/sonicate briefly, avoid prolonged heating.
Assay dilution: Add DMSO stocks to buffered media to reach desired final concentrations while maintaining DMSO within assay tolerance.
Stability checks: Monitor for degradation by LC–MS/HPLC under assay temperature (room temperature to 37°C) and light exposure; store working solutions on ice and protect from light if instability is suspected.
If you intend to use this molecule in chemical reactions (e.g., derivatization), establish all conditions de novo based on the confirmed structure and functional groups from the CoA or independent literature.
Safety and Handling
Safety information specific to this item is not fully provided in the listing and may vary by lot/form.
GHS classification, pictograms, signal word, H-statements: Not specified for this item; refer to the SDS.
Primary storage condition: Store at −20°C (per Product Data). Minimize freeze–thaw cycles; keep container tightly closed under inert headspace if feasible.
Handle in a fume hood; avoid inhalation of dust/aerosols and skin/eye contact.
Recommended PPE: lab coat, safety glasses, and appropriate chemical-resistant gloves (e.g., nitrile). Change gloves regularly when preparing DMSO stocks.
Avoid contact with strong oxidizers or strong acids/bases until compatibility is confirmed from SDS/structure.
Use dry tools for weighing; promptly recap vials to limit moisture uptake and photodegradation.
First aid overview (consult SDS for authoritative guidance)
Skin/eye contact: Rinse with water for ≥15 min. Remove contaminated clothing. Seek medical attention as needed.
Inhalation: Move to fresh air; obtain medical attention if symptoms persist.
Ingestion: Rinse mouth; do not induce vomiting; seek medical help.
Waste and decontamination
Collect solid waste and DMSO/organic solutions in labeled hazardous waste containers per institutional and local regulations. Decontaminate surfaces with suitable solvent/detergent compatible with your workspace materials.
Solvent Selection
Given the product’s use as a small-molecule research tool, solvent choice typically prioritizes solubility, assay compatibility, and chemical stability.
Primary stock solvent (practice): Anhydrous DMSO is the default for many screening compounds due to broad solvency and compatibility with aqueous assay dilution.
Secondary solvents: Depending on structure, acetonitrile (MeCN), methanol/ethanol, or DMF can be used. For less polar scaffolds, isopropanol or MTBE/ethyl acetate may assist in processing but are less common for assay stocks.
Aqueous buffers: Direct dissolution is structure-dependent. Frequently, DMSO stocks are diluted into PBS, HEPES, or Tris buffers. Maintain final DMSO content within assay tolerance (commonly 0.1–1% v/v).
Practical tips
Perform a small-scale solubility screen (DMSO, MeCN, MeOH, buffer) to identify the highest-concentration stable stock without precipitation upon aqueous dilution.
Warm gently (25–37°C) and vortex/sonicate to aid dissolution; avoid prolonged heating.
Filter sterilize (0.22 µm PTFE) only if necessary; adsorption losses may occur.
Comparison (general)
DMSO vs MeCN: DMSO offers higher solubility for polar/heteroatom-rich molecules and is better tolerated in biochemical assays; MeCN provides faster drying for analytics but can denature proteins upon dilution.
Alcohols vs DMF: Alcohols are greener and easier to remove; DMF offers stronger solvency but raises toxicity and disposal considerations.
Storage and Reconstitution
Storage conditions: Store at −20°C (per Product Data). Keep container tightly closed, desiccated, and protected from light. Where possible, maintain an inert headspace (e.g., nitrogen/argon) to limit oxidative degradation.
Shipping: Supplied under cold chain (ice chest + ice pads) to preserve integrity.
Reconstitution and aliquoting (general best practice)
Equilibrate the sealed vial to room temperature before opening to prevent moisture condensation.
Prepare concentrated DMSO stocks (e.g., 10–50 mM). If solubility is uncertain, start at lower concentrations and adjust.
Aliquot stocks into single-use volumes (e.g., 10–50 µL) in low-bind tubes to minimize freeze–thaw.
Store aliquots at −20°C or colder. Avoid repeated freeze–thaw; discard any aliquot showing precipitation or discoloration upon thawing.
In-use stability
Working dilutions in aqueous buffers are typically used immediately; if storage is required, keep on ice and assess stability by LC–MS/HPLC over the intended use period.
Specifications
Appearance, water content, salt form, and exact purity are not specified for this item; refer to CoA/Spec Sheet. If your workflow requires special conditions (anhydrous handling, light protection), validate on receipt and document in your SOPs.
Structure and Identity
Ecopladib (PLA 725) is listed as a small-molecule entry in Aladdin’s Moligand™ collection. The structural identifiers for this catalog entry are incomplete in the provided data.
SKU: E651115
Product name: Ecopladib (PLA 725)
CAS: 381683-92-7 (as provided)
CID: 204106 (as provided)
InChIKey: Not specified for this item; refer to CoA/Spec Sheet. (Provided string "86550" is not a valid InChIKey format.)
SMILES: Not specified for this item; refer to CoA/Spec Sheet.
Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
Structural features
Item-specific 2D/3D structural details are not disclosed in this listing. For definitive structural representation and stereochemical information, consult the certificate of analysis (CoA), specification sheet, or authoritative databases via the CAS/CID provided.
Notes for users
When preparing assay documentation, cite the CAS and SKU alongside your internal batch/lot number to ensure traceability.
If you require canonical SMILES/InChI for cheminformatics or registration, request the latest CoA/SDS package for your lot or verify via a trusted structure database corresponding to the CAS number above.
Synthetic Utility
This product is supplied as a finished small molecule for research use and is not positioned as a general synthetic reagent or building block.
Without disclosed structure, planning derivatizations or conjugations (e.g., linker installation, affinity tags, fluorescent labels) is not supported by this catalog entry. If your workflow requires a derivatized analog, request structural information via CoA or consider de novo synthesis guided by published structures (if available through independent literature sources).
If a functional handle is known from your own records (e.g., phenol, amine, carboxylate), standard transformations (amide coupling, carbamate formation, click chemistry) may be designed accordingly; ensure compatibility with the molecule’s stability and protect sensitive motifs.
Bottom line
Treat this item as an end-use research compound rather than a reagent for broad synthetic applications. For custom analog generation, contact technical support with your structure requirements or pursue custom synthesis based on verified identifiers.
Target Specificity
Target, pathway, or epitope specificity is not provided for this catalog entry. No antigen/epitope, clone, or species reactivity information is applicable, as this product is a small molecule rather than an antibody or protein reagent.
Users should determine any target interactions empirically using validated assays and appropriate positive/negative controls.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
