Edrecolomab ELISA Kit

Cat. No.: EJ1515744

GRADE & PURITY BioReagent

Descripción general

This kit employs the indirect enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human CD326 antigen. The sample or standard is first added to the wells, followed by an HRP (horseradish peroxidase)-labeled secondary antibody. The procedure involves incubation and wash steps between each addition. TMB substrate is then added for color development. Under the catalysis of the peroxidase (HRP), TMB is converted into a blue product, which subsequently turns into a final stable yellow color upon the addition of an acid stop solution. The intensity of the yellow color is directly proportional to the concentration of Edrecolomab in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.

Specifications

Especificaciones y pureza

BioReagent

Grado

BioReagent

Aplicación

ELISA

Tipo de muestra

homogenados de tejido, Lisado celular, Plasma, sobrenadantes de cultivos celulares, Suero

Rango de detección

0.78ng/mL~50ng/mL

Especie detectada Objeto

Human

Especificidad

Detecting Human Edrecolomab in samples, with no significant cross-reactivity to other homologs.

Detection instrument

Microplate reader

Detection wavelength

450nm

Repeatability

The intra- and inter-assay coefficients of variation (CV) are both <10%.

Detection duration

3.5 hours, not including equilibration and sample preparation time.

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Estabilidad y almacenamiento

Store at 2-8℃ for 6 months.