Electrophoresis Sample Buffer

Cat. No.: E493020

Storage

Store at 2-8°C

Shipped In

Wet ice

Descripción general

Electrophoresis Sample Buffer, is a ready-to-use reducing electrophoresis sample buffer solution with bromophenol blue for the preparation of protein samples to be separated in SDS-PAGE. Bromophenol Blue is included with Electrophoresis Sample Bufferas a tracking dye to monitor the sample during the electrophoretic run. Electrophoresis Sample Buffer,  contains DTT (sc-29089), which disrupts disulfide bonds to ensure proteins are fully denatured before loading on the gel. Electrophoresis Sample Buffer also contains glycerol, TCEP, DTT, SDS, 1.0M Tris-HCl pH 6.7 and bromophenol blue. For non-reducing SDS-PAGE, Electrophoresis Sample Buffer (non-reducing) (sc-45085) is recommended.
Mix sample with an equal volume of Electrophoresis Sample Buffer, .Bring to 95° C for 10 minutes, cool to room temperature before loading. If particulate is present, centrifuge samples 5 minutes at 14k RPM in microcentrifuge, and load the gel.
Electrophoresis Sample Buffer is a ready-to-use reducing sample buffer for SDS-PAGE, acting on denatured proteins in the system.

Electrophoresis Sample Buffer, 2X (non-reducing) is a ready-to-use non-reducing electrophoresis sample buffer solution with bromophenol blue for the preparation of protein samples to be separated in non-reducing gels. Non-reducing SDS-PAGE (no boiling and no reducing agent) is used when the properties of native proteins are being analyzed.|Contains glycerol, 0.217 M Tris-HCl pH 6.8 and bromophenol blue.|For reducing SDS-PAGE, Electrophoresis Sample Buffer 2X (sc-24945) is recommended.
Electrophoresis Sample Buffer, 2X (non-reducing)is a ready-to-use, non-reducing sample buffer solution

Product specifications and parameters

sku	name	Concentration multiple	pack size
E493020-01-1L	Electrophoresis Sample Buffer	2×	1L
E493020-01-25ml	Electrophoresis Sample Buffer	2×	25ml
E493020-02-25ml	Electrophoresis Sample Buffer	2×(non-reducing)	25ml

Especificaciones

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Preguntas frecuentes

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.