Specific Activity ＞25 U/mg;Activity ＞5 U/ml for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Product Description
Endo F3, Endoglycosidase F3, endo-beta-N-acetylglucosaminidase F
Endo F3 cleaves free or Asparagine-linked triantennary or alpha-(1-6) fucosylated biantennary oligosaccharides,as well as triamnnosyl chitobiose core structures. Nonfucosylated biantennary glycans will also be cleaved, but at a 40x reduced rate. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact. Alpha 1-3 fucosylation will inhibit enzymatc activity. There is no activity on oligomannose and hybrid molecules.
Source recombinant Elizabethkingia meningosepticum (was Chryseobacterium meningosepticum)
EC 3.2.1.96
Specific Activity >25 U/mg
Activity >5 U/ml
Molecular weight 30,000 daltons
Specific Activity
Defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of porcine fibrinogen in 1 minute at 37˚C, pH 4.5. Cleavage is monitored by SDS-PAGE (cleaved fibrinogen migrates faster).
Specifictity
Cleaves all asparagine-linked triantennary or alpha-(1-6)-fucosylated biantennary oligosaccharides, as well as triamannosyl chitobiose core structures. Alpha 1-3 fucosylation will inhibit enzymatic activity. Nonfucosylated biantennary glycans will also be cleaved, but at a 40x reduced rate. (Note:The recombinant version is not glycosylated, which may result in properties differing from the native protein.)
Contents
60 µl aliquot of enzyme (0.3 U) in 20 mM Tris-HCl, pH 7.5
5x Reaction Buffer – 250 mM sodium acetate, pH 4.5
Suggested usage
1. Add up to 200 µg of glycoprotein to an Eppendorf tube. Adjust to 38 µl final volume with de-ionized water.
2. Add 10 µl 5x Reaction Buffer 4.5
4. Add 2.0 µl of Endo F3. Incubate 1 hour at 37˚C.
Storage
Store enzyme at 4˚C.
Stability Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
Purity Endoglycosidase F3 is tested for contaminating protease as follows; 10 μg of denatured BSA is incubated for 24 hours at 37oC with 2 μL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
The production host strain has been extensively tested and does not produce any detectable glycosidases.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
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