FAM120A/OSSA Mouse mAb - Anticuerpo primario, specific to FAM120A

Cat. No.: Ab102814

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, IHC, WB

Host species

Ratón

Reactive species

Humano

Isotype

Mouse IgG1

Clone Number

F01S803

Conjugation

Unconjugated

Descripción general

Purity

Immunogen affinity purified

Tested applications

Suitable for: ICC/IF , IHC-P , WB more details

Function

May participate in mRNA transport in the cytoplasm (By similarity). Critical component of the oxidative stress-induced survival signaling. Activates src family kinases and acts as a scaffolding protein enabling src family kinases to phosphorylate and activate PI3-kinase. Binds RNA and promotes the secretion of IGF-II. May play a pivotal role in the progression of scirrhous-type gastric cancer by supporting cancer cell survival in environments with various oxidative stresses.

Post-translational

Arg-982 is dimethylated, probably to asymmetric dimethylarginine. Phosphorylated on tyrosine by src family kinases upon ultraviolet exposure.

Specifications

Product Name

FAM120A/OSSA Mouse mAb - Anticuerpo primario, specific to FAM120A

Sinónimos

C9orf10 antibody | Chromosome 9 open reading frame 10 antibody | Constitutive coactivator of PPAR gamma like protein 1 antibody | Constitutive coactivator of PPAR-gamma-like protein 1 antibody | DNA polymerase transactivated protein5 antibody | DNA polyme

Especificaciones y pureza

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Especies hospedadoras

Ratón

Especificidad

FAM120A

Inmunógeno

Recombinant fragment within Human FAM120A/OSSA (internal sequence). The exact sequence is proprietary.

Control positivo

WB: MCF7 and SK-N-SH cell lysates. IHC: Human brain tissue. IF/ICC: HeLa cells.

Conjugación

Unconjugated

Grado

Carrier Free, ExactAb™, Validated

Tipo de cadena ligera

kappa

SDS-PAGE

150 kDa

Método de purificación

Protein G purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Forma

Liquid

Condiciones de almacenamiento de almacenamiento

Store at -20°C,Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

Estabilidad y almacenamiento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imágenes

Western Blot: FAM120A/OSSA Mouse mAb (Ab102814)

FAM120A/OSSA Mouse mAb (Ab102814) - Western Blot
All lanes: FAM120A/OSSA Mouse mAb (Ab102814) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/30000 dilution
Predicted band size: 121 kDa
Observed band size: 145 kDa
Exposure time: 100.0 s

IHC: FAM120A/OSSA Mouse mAb (Ab102814)

FAM120A/OSSA Mouse mAb (Ab102814) - IHC
Immunohistochemical analysis of FAM120A staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with FAM120A/OSSA Mouse mAb (Ab102814) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: FAM120A/OSSA Mouse mAb (Ab102814)

FAM120A/OSSA Mouse mAb (Ab102814) - IF/ICC
Immunofluorescent analysis of FAM120A staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with FAM120A/OSSA Mouse mAb (Ab102814) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Aplicación

• Aplicación: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/50-1/200;

• IF/ICC:

  1/10 - 1/50 ;

Objetivos asociados (humanos)

FAM120A Tbio Constitutive coactivator of PPAR-gamma-like protein 1 (0 Activities)

Discover Target: FAM120A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de acción

Preguntas frecuentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (FAM120A/OSSA Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.