Laboratory Protocols & SOPs Library

Antibiotic Medium #10 (Powder)

Directions per Liter:

Dissolve 52 grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.


Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).


Component g/liter
Pancreatic Digest of Casein 17
Dextrose 2.5
Agar 12
Sodium Chloride 5
Papaic Digest of Soybean Meal 3
Potassium Phosphate, Dibasic 2.5
Tween 80 10
Total: 52g/Liter


Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons.   Mw — weight-average molecular weight.   Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Cite this article

Aladdin Scientific. "Antibiotic Medium #10 (Powder)" Aladdin Knowledge Base, updated 9 jun 2025. https://www.aladdinsci.com/eu_es/faqs/antibiotic-medium-10-powder-en.html
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