BCA concentration measured protein concentration 3mg/ml, but after adding buffer found no protein bands appeared, what is the reason?
BCA concentration measured protein concentration 3mg/ml, but after adding buffer found no protein bands appeared, what is the reason?
posted this at Jun 9, 2025 Updated at Aug 27, 2024
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BCA concentration measured protein concentration 3mg/ml, but after adding buffer found no protein bands appeared, what is the reason?
It is recommended to conduct a comparison experiment, set BSA as a positive control, and add buffer at the same time as the protein sample lysate to mix and boil the denaturation, on the sample for electrophoresis to observe the bands.
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