Blood Agar Base No. 2 (Powder)

Directions per Liter:

Dissolve 42grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized. Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi). Prepare 5 to 10% blood agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium. Cool to 45-50°C. Dispense into Petri dishes or tubes.

Component	g/liter
Casein Digest Peptone	7.5
Liver Digest	5
Peptic Digest of Animal Tissue	7.5
Sodium Chloride	5
Yeast Extract	5
Agar	12
	Total: 42g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.