Brucella Broth (Powder)

Directions per Liter:

Mix 28.0g per liter of ddH2O until evenly dispersed. Heat with stirring if necessary to dissolve completely. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Component	g/liter
Casein Digest Peptone	10
Dextrose	1
Peptic Digest of Animal Tissue	10
Sodium Chloride	5
Sodium Bisulfite	0.1
Yeast Extract	2
 	Total: 28g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.