Detection of activated platelets by flow cytometry

Summary

This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.

Operation method

Staining of activated platelets for double labeling detection by whole blood method

Materials and Instruments

Whole blood
Citrate or hirudin CD62p-FlTC CD42b-PE PBS

Move

1. Collect blood citrate or hirudin anticoagulation.

2. 10 ul CD62p-FlTC plus 10 ul CD42b-PE, plus 5 ul whole blood (sample tube); 10 ul IgG1-FITC plus 10 ul IgG1-PE, plus 5 ul whole blood (control tube); mix gently and incubate at room temperature for 15 min.

3. Add 2~3 ml PBS (1% PFA) Terminate the reaction and analyze the platelets by SSC-LOG/CD42b-PE circling.

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