Familiarize yourself with the principles of isolation and identification of pathogenic Enterobacteriaceae in feces.
Operation method
Stool specimens or anal swabs
Materials and Instruments
Chinese orchid agar plate or S-S agar plate or EMB plate Move Caveat Enrichment culture if necessary. For more product details, please visit Aladdin Scientific website.
1. Pick a small amount of specimen with a sterile inoculating loop and inoculate in a line on a Chinese orchid agar plate or S-S agar plate. (If necessary, incubate for 6 to 12 hours before isolation).
2. Use a sterile inoculating needle to select colorless, transparent and small suspicious colonies from the selective medium and inoculate them on semi-solid disaccharide iron-containing medium for pure culture and preliminary identification.
3. Based on the preliminary identification of the growth in the semi-solid disaccharide-containing iron medium, conduct other biochemical reaction tests (monosaccharide fermentation, indigo substrate test, etc.), and then conduct slide agglutination test with diagnostic serum to make the final identification.