Hybridization and gene localization experiments in Escherichia coli
Hybridization and gene localization experiments in Escherichia coli
posted this at Jun 9, 2025 Updated at Dec 24, 2024
Summary
The E. coli chromosome is ring-shaped. High-frequency recombinant strains (Hfr) have the F factor integrated into their chromosomes, and the chromosomes of the Hfr cells (donor bacteria) are transferred from the Hfr cells into the F- cells when the Hfr bacteria undergo splicing (i.e., hybridization) with the F- bacterial cells.
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