Liquid medium transfer technique

Summary

Liquid media transfer techniques are used for (1) enrichment and observation of pure species of bacteria (2) characterization of bacterial growth in liquid environments (turbid growth, surface growth, or sedimentation growth).

Operation method

Liquid medium transfer technique

Principle

Due to the diversity of microbial species and metabolic types, there are many types of culture media used for the cultivation of microorganisms, and their formulas and preparation methods are different. However, the procedures for preparing culture media are generally the same, such as the preparation of vessels, the preparation and dispensing of culture media, the making of cotton plugs, the sterilizing of culture media, the making of slants and plates, and the aseptic checking of the culture media, etc. The basic procedures are generally similar.

Materials and Instruments

Bacillus subtilis Escherichia coli Streptococcus pyogenes
Broth media
Inoculation rings Thermostats Test tubes

Move

1. Take the inoculation ring, sterilize it by burning on the flame and cool it down.

2, "double tube transplantation method" left hand holding bacterial slant culture and broth medium two test tubes, the right hand holding the inoculation ring, according to the aseptic operation method to take a small amount of bacteria, will be stained with bacteria inoculation ring in the slanting close to the liquid surface of the wall of the tube gently smeared with the research, the test tube upright so that the bacteria on the wall didn't fall into the liquid, put in the inoculation after 37 ℃ constant temperature box After inoculation, put it into 37℃ constant temperature box for cultivation.

Caveat

1. The correct hand position for inoculation of bacteria is to hold the bottom of the plate with bacteria in the left hand and the inoculation ring in the right hand.

2. Remove the inoculation ring and sterilize the mouth of the tube by passing it through a flame.

3. After inoculation, incubate at 37°C in a thermostat.

Common Problems

Results Analysis:

1, turbid growth of Escherichia coli bacterial fluid was uniformly turbid, with a small amount of precipitate at the bottom of the tube.

2, precipitation growth of Streptococcus pyogenes bacterial fluid at the bottom of the tube with precipitation, the bacterial fluid without obvious turbidity.

3、Bacterial membrane growth of Bacillus subtilis bacterial solution to form a membrane on the surface.

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